Role of cell-cell interactions in high mobility group box 1 cytokine activity in human peripheral blood mononuclear cells and mouse splenocytes

Hideo Kohka Takahashi, Hiroshi Sadamori, Keyue Liu, Hidenori Wake, Shuji Mori, Tadashi Yoshino, Yasuhiko Yamamoto, Hiroshi Yamamoto, Masahiro Nishibori

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Cell-cell interaction through binding of intercellular adhesion molecule (ICAM), B7.1, B7.2 and CD40 on monocytes to their ligands on T-cells plays a number of roles in cytokine. High mobility group box 1 (HMGB1), an abundant and conserved nuclproduction and lymphocyte proliferationear protein, acts in the extracellular environment as a primary pro-inflammatory signal. The receptor for advanced glycation end products (RAGE), toll-like receptor (TLR)-2 and TLR-4 are receptors for HMGB1. HMGB1 induces pro-inflammatory cytokine production in monocytes and T-cells. This study was designed to study the cellular mechanism of cytokine production. HMGB1 concentration-dependently induced ICAM-1, B7.1, B7.2 and CD40 expression on monocytes, and interferon (IFN)-γ and tumor necrosis factor (TNF)-α production and lymphocyte proliferation in human peripheral blood mononuclear cells (PBMCs). These HMGB1 activities depended on the stimulation of RAGE on monocytes. HMGB1 also up-regulated RAGE, but not TLR-2 or TLR-4, expression on monocytes, which was inhibited by antibodies (Abs) against ICAM-1, B7.1, B7.2 and CD40. These results together indicated that HMGB1 could induce an intimate cellular interplay between monocytes and T-cells in PBMCs through the stimulation and up-regulation of RAGE and other adhesive molecules on monocytes.

Original languageEnglish
Pages (from-to)194-202
Number of pages9
JournalEuropean Journal of Pharmacology
Volume701
Issue number1-3
DOIs
Publication statusPublished - Feb 15 2013

Keywords

  • Adhesion molecules
  • High mobility group box 1
  • Monocytes/macrophages
  • Receptor for advanced glycation end products
  • T-cells
  • Toll-like receptor

ASJC Scopus subject areas

  • Pharmacology

Fingerprint Dive into the research topics of 'Role of cell-cell interactions in high mobility group box 1 cytokine activity in human peripheral blood mononuclear cells and mouse splenocytes'. Together they form a unique fingerprint.

  • Cite this