Role of calcium conductances on spike afterpotentials in rat trigeminal motoneurons

Masayuki Kobayashi, Tomio Inoue, Ryuji Matsuo, Yuji Masuda, Osamu Hidaka, Youngnam Kang, Toshifumi Morimoto

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Abstract

Intracellular recordings were obtained from rat trigeminal motoneurons in slice preparations to investigate the role of calcium conductances in the depolarizing and hyperpolarizing spike afterpotential (ADP and mAHP, respectively). The mAHP was suppressed by bath application of 1 μM apamin, 2 mM Mn2+, and 2 mM Co2+, and also by intracellular injection of ethylene glycol-bis(b-aminoethylenether)-N,N,N',N'-tetraacetic acid (EGTA), suggesting that the potassium conductance generating the mAHP is activated by Ca2+ influx. Mn2+ (2 mM) or Cd2+ (500 μM) reduced the ADP, whereas the ADP amplitude was increased by raising extracellular Ca2+ concentration from 2 to 8 mM by bath application of Ba2+ (0.5-5 mM) and by intracellular injection of EGTA. This would suggest that Ca2+ itself is likely to be the charge carrier generating the ADP. Focal application of ω-conotoxin GVIA (10-30 μM) suppressed the mAHP and enhanced the ADP, whereas focal application of ω-agatoxin IVA (10-100 μM) reduced the ADP amplitude without apparent effects on the mAHP. We conclude that Ca2+ influx through ω- agatoxin IVA-sensitive calcium channels is at least in part responsible for the generation of the ADP and that Ca2+ influx through ω-conotoxin GVIA- sensitive calcium channels contributes to the generation of the mAHP. Because of the selective suppression of the ADP and mAHP by ω-agatoxin IVA and ω- conotoxin GVIA, respectively, it is suggested that both calcium channels are separated geometrically in rat trigeminal motoneurons.

Original languageEnglish
Pages (from-to)3273-3283
Number of pages11
JournalJournal of neurophysiology
Volume77
Issue number6
DOIs
Publication statusPublished - Jan 1 1997

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ASJC Scopus subject areas

  • Neuroscience(all)
  • Physiology

Cite this

Kobayashi, M., Inoue, T., Matsuo, R., Masuda, Y., Hidaka, O., Kang, Y., & Morimoto, T. (1997). Role of calcium conductances on spike afterpotentials in rat trigeminal motoneurons. Journal of neurophysiology, 77(6), 3273-3283. https://doi.org/10.1152/jn.1997.77.6.3273