Role of Arg-72 of pharaonis Phoborhodopsin (Sensory Rhodopsin II) on its Photochemistry

Yukako Ikeura, Kazumi Shimono, Masayuki Iwamoto, Yuki Sudo, Naoki Kamo

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Pharaonis phoborhodopsin (ppR, or pharaonis sensory rhodopsin II, NpsRII) is a sensor for the negative phototaxis of Natronomonas (Natronobacterium) pharaonis. Arginine 72 of ppR corresponds to Arg-82 of bacteriorhodopsin, which is a highly conserved residue among microbial rhodopsins. Using various Arg-72 ppR mutants, we obtained the following results: 1), Arg-72ppR together possibly with Asp-193 influenced the pKa of the counterion of the protonated Schiff base. 2), The M-rise became approximately four times faster than the wild-type. 3), Illumination causes proton uptake and release, and the pH profiles of the sequence of these two proton movements were different between R72A mutant and the wild-type; it is inferred that Arg-72 connects the proton transfer events occurring at both the Schiff base and an extracellular proton-releasing residue (Asp-193). 4), The M-decays of Arg-72 mutants were faster (∼8-27 folds at pH 8 depending on mutants) than the wild-type, implying that the guanidinium prevents the proton transfer from the extracellular space to the deprotonated Schiff base. 5), The proton-pumping activities were decreased for mutants having increased M-decay rates, but the extent of the decrease was smaller than expected. The role of Arg-72 of ppR on the photochemistry was discussed.

Original languageEnglish
Pages (from-to)3112-3120
Number of pages9
JournalBiophysical Journal
Volume86
Issue number5
Publication statusPublished - May 2004
Externally publishedYes

Fingerprint

Sensory Rhodopsins
Photochemistry
Protons
Schiff Bases
Natronobacterium
Microbial Rhodopsins
Bacteriorhodopsins
Guanidine
Extracellular Space
Lighting
Arginine

ASJC Scopus subject areas

  • Biophysics

Cite this

Ikeura, Y., Shimono, K., Iwamoto, M., Sudo, Y., & Kamo, N. (2004). Role of Arg-72 of pharaonis Phoborhodopsin (Sensory Rhodopsin II) on its Photochemistry. Biophysical Journal, 86(5), 3112-3120.

Role of Arg-72 of pharaonis Phoborhodopsin (Sensory Rhodopsin II) on its Photochemistry. / Ikeura, Yukako; Shimono, Kazumi; Iwamoto, Masayuki; Sudo, Yuki; Kamo, Naoki.

In: Biophysical Journal, Vol. 86, No. 5, 05.2004, p. 3112-3120.

Research output: Contribution to journalArticle

Ikeura, Y, Shimono, K, Iwamoto, M, Sudo, Y & Kamo, N 2004, 'Role of Arg-72 of pharaonis Phoborhodopsin (Sensory Rhodopsin II) on its Photochemistry', Biophysical Journal, vol. 86, no. 5, pp. 3112-3120.
Ikeura, Yukako ; Shimono, Kazumi ; Iwamoto, Masayuki ; Sudo, Yuki ; Kamo, Naoki. / Role of Arg-72 of pharaonis Phoborhodopsin (Sensory Rhodopsin II) on its Photochemistry. In: Biophysical Journal. 2004 ; Vol. 86, No. 5. pp. 3112-3120.
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