Role of acidic amino acid for regulating hydroxyapatite crystal growth

Takuya Matsumoto, Masayuki Okazaki, Masahiro Inoue, Jun Ichi Sasaki, Yoshinosuke Hamada, Junzo Takahashi

Research output: Contribution to journalArticlepeer-review

26 Citations (Scopus)


Non-collagenous proteins in hard tissue matrix are thought to play a pivotal role in regulating the crystal growth of hydroxyapatite (HAp). As most non-collagenous proteins are acidic proteins containing acidic amino acid-rich sequences, we examined the growth of HAp crystals from HAp seed crystals in the presence/absence of acidic amino acid. New HAp formation generally started from the P-surface of HAp. However, in the presence of acidic amino acid, new HAp formation was observed on both P-surface and C-surface of HAp seed crystals. Furthermore, newly formed HAp showed specific orientation along the long-axis direction of HAp seed crystals. In terms of crystallinity, HAp formed in the presence of acidic amino acid showed low crystallinity. These results suggested that, in biomineralization, the adsorbed or free state of acidic amino acid would influence crystal formation and orientation as follows: 1) If free in solution, acidic amino acid would inhibit HAp crystal growth; 2) If adsorbed or immobilized on matrix, acidic amino acid would become HAp nucleation site and regulate the orientation of HAp crystals.

Original languageEnglish
Pages (from-to)360-364
Number of pages5
JournalDental materials journal
Issue number2
Publication statusPublished - Jun 2006
Externally publishedYes


  • Acidic amino acid
  • Crystal growth
  • Hydroxyapatite

ASJC Scopus subject areas

  • Ceramics and Composites
  • Dentistry(all)


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