Role of α-tocopherol in the regulation of mitochondrial permeability transition

Masae Yorimitsu, Shikibu Muranaka, Eisuke F. Sato, Hirofumi Fujita, Kouichi Abe, Tatsuji Yasuda, Masayasu Inoue, Kozo Utsumi

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

We previously showed that Ca2+-induced cyclosporin A-sensitive membrane permeability transition (MPT) of mitochondria occurred with concomitant generation of reactive oxygen species (ROS) and release of cytochrome c (Free Rad. Res.38, 29-35, 2004). To elucidate the role of α-tocopherol in MPT, we investigated the effect of α-tocopherol on mitochondrial ROS generation, swelling and cytochrome c release induced by Ca2+ or hydroxyl radicals. Biochemical analysis revealed that α-tocopherol suppressed Ca2+-induced ROS generation and oxidation of critical thiol groups of mitochondrial adenine nucleotide translocase (ANT) but not swelling and cytochrome c release. Hydroxyl radicals also induced cyclosporin A-sensitive MPT of mitochondria. α-Tocopherol suppressed the hydroxyl radical-induced lipid peroxidation, swelling and cytochrome c release from mitochondria. These results indicate that α-tocopherol inhibits ROS generation, ANT oxidation, lipid peroxidation and the opening of MPT, thereby playing important roles in the prevention of oxidative cell death.

Original languageEnglish
Pages (from-to)95-107
Number of pages13
JournalPhysiological Chemistry and Physics and Medical NMR
Volume36
Issue number2
Publication statusPublished - 2004
Externally publishedYes

Fingerprint

Tocopherols
Permeability
Cytochromes c
Mitochondria
Reactive Oxygen Species
ATP Translocases Mitochondrial ADP
Hydroxyl Radical
Membranes
Swelling
Lipid Peroxidation
Cyclosporine
Lipids
Oxidation
Cell death
Sulfhydryl Compounds
Cell Death

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)
  • Biophysics
  • Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Radiological and Ultrasound Technology
  • Radiology Nuclear Medicine and imaging

Cite this

Yorimitsu, M., Muranaka, S., Sato, E. F., Fujita, H., Abe, K., Yasuda, T., ... Utsumi, K. (2004). Role of α-tocopherol in the regulation of mitochondrial permeability transition. Physiological Chemistry and Physics and Medical NMR, 36(2), 95-107.

Role of α-tocopherol in the regulation of mitochondrial permeability transition. / Yorimitsu, Masae; Muranaka, Shikibu; Sato, Eisuke F.; Fujita, Hirofumi; Abe, Kouichi; Yasuda, Tatsuji; Inoue, Masayasu; Utsumi, Kozo.

In: Physiological Chemistry and Physics and Medical NMR, Vol. 36, No. 2, 2004, p. 95-107.

Research output: Contribution to journalArticle

Yorimitsu, M, Muranaka, S, Sato, EF, Fujita, H, Abe, K, Yasuda, T, Inoue, M & Utsumi, K 2004, 'Role of α-tocopherol in the regulation of mitochondrial permeability transition', Physiological Chemistry and Physics and Medical NMR, vol. 36, no. 2, pp. 95-107.
Yorimitsu, Masae ; Muranaka, Shikibu ; Sato, Eisuke F. ; Fujita, Hirofumi ; Abe, Kouichi ; Yasuda, Tatsuji ; Inoue, Masayasu ; Utsumi, Kozo. / Role of α-tocopherol in the regulation of mitochondrial permeability transition. In: Physiological Chemistry and Physics and Medical NMR. 2004 ; Vol. 36, No. 2. pp. 95-107.
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