Role of α-tocopherol in the regulation of mitochondrial permeability transition

Masae Yorimitsu, Shikibu Muranaka, Eisuke F. Sato, Hirofumi Fujita, Kouichi Abe, Tatsuji Yasuda, Masayasu Inoue, Kozo Utsumi

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)

Abstract

We previously showed that Ca2+-induced cyclosporin A-sensitive membrane permeability transition (MPT) of mitochondria occurred with concomitant generation of reactive oxygen species (ROS) and release of cytochrome c (Free Rad. Res.38, 29-35, 2004). To elucidate the role of α-tocopherol in MPT, we investigated the effect of α-tocopherol on mitochondrial ROS generation, swelling and cytochrome c release induced by Ca2+ or hydroxyl radicals. Biochemical analysis revealed that α-tocopherol suppressed Ca2+-induced ROS generation and oxidation of critical thiol groups of mitochondrial adenine nucleotide translocase (ANT) but not swelling and cytochrome c release. Hydroxyl radicals also induced cyclosporin A-sensitive MPT of mitochondria. α-Tocopherol suppressed the hydroxyl radical-induced lipid peroxidation, swelling and cytochrome c release from mitochondria. These results indicate that α-tocopherol inhibits ROS generation, ANT oxidation, lipid peroxidation and the opening of MPT, thereby playing important roles in the prevention of oxidative cell death.

Original languageEnglish
Pages (from-to)95-107
Number of pages13
JournalPhysiological chemistry and physics and medical NMR
Volume36
Issue number2
Publication statusPublished - 2004
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Physiology
  • Spectroscopy

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