Role for complement receptors (CD21/CD35) in the regulation of recombination activating gene expression in murine peripheral B cells

Hitoshi Ohmori, Masaki Magari, Yasunori Nakayama, Naoki Kanayama, Masaki Hikida

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

A population of peripheral B cells have been shown to express recombination activating gene products, RAG-1 and RAG-2, which are considered to be involved in revising the B cell antigen receptor (BCR) in the periphery. BCR engagement has been reported to turn off RAG expression in peripheral B cells, whereas the same treatment has an opposite effect on immature B cells in the bone marrow. In contrast to receptor editing that is involved in the removal of autoreactivity in immature B cells, it has been shown that secondary V(D)J rearrangement in peripheral B cells, termed receptor revision, contributes to affinity maturation of antibodies. Here, we show that RAG-2 expression in murine splenic B cells was abrogated by the coligation of BCR with complement receptors (CD21/CD35) much more efficiently than by the engagement of BCR alone. On the other hand, the same coligation augmented proliferation of anti-CD40-stimulated B cells. These findings suggest a crucial role for CD21/CD35 in directing the conservation or the revision of BCRs in peripheral B cells.

Original languageEnglish
Pages (from-to)95-99
Number of pages5
JournalImmunology Letters
Volume83
Issue number2
DOIs
Publication statusPublished - Sep 2 2002

Keywords

  • Complement receptors
  • Periperal B cells
  • V(D)J recombination

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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