RNA-Seq using bulked recombinant inbred line populations uncovers the importance of brassinosteroid for seed longevity after priming treatments

Naoto Sano, June Sik Kim, Yoshihiko Onda, Takahito Nomura, Keiichi Mochida, Masanori Okamoto, Mitsunori Seo

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Seed priming is a commercially used technique for improving seed performance including germination. However, the treatment sometimes reduces seed longevity as a side effect, limiting the storable period or longevity of the seeds. To overcome this problem, molecular mechanisms involved in the loss of seed longevity during priming were analyzed using natural variations of Arabidopsis thaliana. We found that the Est-1 accession retained longevity for longer after priming compared to the reference accession Col-0. QTL analysis using 279 recombinant inbred lines (RILs) derived from the Est-1 × Col-0 detected three QTL regions associated with the loss of seed longevity during priming. Bulked transcriptome analysis (RNA-Seq with bulked RIL populations) revealed that genes related to brassinosteroid (BR) biosynthesis/signaling and cell wall modification were highly expressed in primed seeds with shorter longevity. After priming, BR-deficient mutants cyp85a1/a2 and det2 showed significantly longer longevity than the wild type (WT). Moreover, tetrazolium staining indicated that mutant seed coats were less permeable after priming than those of WT. We suggest that the loss of seed longevity in primed seed is due to increased seed coat permeability, which is positively regulated, at least partly, via BR signaling.

Original languageEnglish
Article number8095
JournalScientific Reports
Volume7
Issue number1
DOIs
Publication statusPublished - Dec 1 2017

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brassinosteroids
inbred lines
RNA
seeds
quantitative trait loci
seed priming
mutants
tetrazolium
transcriptomics
permeability
Arabidopsis thaliana
adverse effects
cell walls
biosynthesis
germination

ASJC Scopus subject areas

  • General

Cite this

RNA-Seq using bulked recombinant inbred line populations uncovers the importance of brassinosteroid for seed longevity after priming treatments. / Sano, Naoto; Kim, June Sik; Onda, Yoshihiko; Nomura, Takahito; Mochida, Keiichi; Okamoto, Masanori; Seo, Mitsunori.

In: Scientific Reports, Vol. 7, No. 1, 8095, 01.12.2017.

Research output: Contribution to journalArticle

Sano, Naoto ; Kim, June Sik ; Onda, Yoshihiko ; Nomura, Takahito ; Mochida, Keiichi ; Okamoto, Masanori ; Seo, Mitsunori. / RNA-Seq using bulked recombinant inbred line populations uncovers the importance of brassinosteroid for seed longevity after priming treatments. In: Scientific Reports. 2017 ; Vol. 7, No. 1.
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