RNA-Seq-based DNA marker analysis of the genetics and molecular evolution of Triticeae species

Kazuhiro Sato, Kentaro Yoshida, Shigeo Takumi

Research output: Contribution to journalReview articlepeer-review

Abstract

The release of high-quality chromosome-level genome sequences of members of the Triticeae tribe has greatly facilitated genetic and genomic analyses of important crops such as wheat (Triticum aestivum) and barley (Hordeum vulgare). Due to the large diploid genome size of Triticeae plants (ca. 5 Gbp), transcript analysis is an important method for identifying genetic and genomic differences among Triticeae species. In this review, we summarize our results of RNA-Seq analyses of diploid wheat accessions belonging to the genera Aegilops and Triticum. We also describe studies of the molecular relationships among these accessions and provide insight into the evolution of common hexaploid wheat. DNA markers based on polymorphisms within species can be used to map loci of interest. Even though the genome sequence of diploid Aegilops tauschii, the D-genome donor of common wheat, has been released, the diploid barley genome continues to provide key information about the physical structures of diploid wheat genomes. We describe how a series of RNA-Seq analyses of wheat relatives has helped uncover the structural and evolutionary features of genomic and genetic systems in wild and cultivated Triticeae species.

Original languageEnglish
Pages (from-to)535-542
Number of pages8
JournalFunctional and Integrative Genomics
Volume21
Issue number5-6
DOIs
Publication statusPublished - Nov 2021

Keywords

  • Aegilops
  • DNA marker
  • Hordeum
  • RNA-Seq
  • Triticeae
  • Triticum

ASJC Scopus subject areas

  • Genetics

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