RNA isolation using immobilized PNA.

Takashi Ohtsuki, Chisato Kumano, Taishi Manabe, Mizuki Kitamatsu, Masahiko Sisido

Research output: Contribution to journalArticle

Abstract

A novel method to isolate a single kind of RNA from RNA mixture was developed by using a biotinylated-PNA (peptide nucleic acid) that is complementary to the RNA. Efficient bindings of Escherichia coli tRNA(Leu)CUC were observed to the 9-mer and 12-mer PNAs that are complementary to the 3'-end region of the tRNA. Purified E. coli tRNA(Leu)CUC was obtained from E. coli tRNA mixture.

Original languageEnglish
Pages (from-to)263-264
Number of pages2
JournalNucleic acids symposium series (2004)
Issue number49
DOIs
Publication statusPublished - 2005

ASJC Scopus subject areas

  • Medicine(all)

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