RNA isolation using immobilized PNA.

Takashi Ohtsuki, Chisato Kumano, Taishi Manabe, Mizuki Kitamatsu, Masahiko Sisido

Research output: Contribution to journalArticle

Abstract

A novel method to isolate a single kind of RNA from RNA mixture was developed by using a biotinylated-PNA (peptide nucleic acid) that is complementary to the RNA. Efficient bindings of Escherichia coli tRNA(Leu)CUC were observed to the 9-mer and 12-mer PNAs that are complementary to the 3'-end region of the tRNA. Purified E. coli tRNA(Leu)CUC was obtained from E. coli tRNA mixture.

Original languageEnglish
Pages (from-to)263-264
Number of pages2
JournalNucleic acids symposium series (2004)
Issue number49
Publication statusPublished - 2005

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RNA, Transfer, Leu
RNA
Escherichia coli
Transfer RNA
Peptide Nucleic Acids
Complementary RNA

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Ohtsuki, T., Kumano, C., Manabe, T., Kitamatsu, M., & Sisido, M. (2005). RNA isolation using immobilized PNA. Nucleic acids symposium series (2004), (49), 263-264.

RNA isolation using immobilized PNA. / Ohtsuki, Takashi; Kumano, Chisato; Manabe, Taishi; Kitamatsu, Mizuki; Sisido, Masahiko.

In: Nucleic acids symposium series (2004), No. 49, 2005, p. 263-264.

Research output: Contribution to journalArticle

Ohtsuki, T, Kumano, C, Manabe, T, Kitamatsu, M & Sisido, M 2005, 'RNA isolation using immobilized PNA.', Nucleic acids symposium series (2004), no. 49, pp. 263-264.
Ohtsuki T, Kumano C, Manabe T, Kitamatsu M, Sisido M. RNA isolation using immobilized PNA. Nucleic acids symposium series (2004). 2005;(49):263-264.
Ohtsuki, Takashi ; Kumano, Chisato ; Manabe, Taishi ; Kitamatsu, Mizuki ; Sisido, Masahiko. / RNA isolation using immobilized PNA. In: Nucleic acids symposium series (2004). 2005 ; No. 49. pp. 263-264.
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