RNA isolation using immobilized PNA.

Takashi Ohtsuki; Chisato Kumano; Taishi Manabe; Mizuki Kitamatsu; Masahiko Sisido

doi:10.1093/nass/49.1.263

RNA isolation using immobilized PNA.

Takashi Ohtsuki, Chisato Kumano, Taishi Manabe, Mizuki Kitamatsu, Masahiko Sisido

Research output: Contribution to journal › Article

Abstract

A novel method to isolate a single kind of RNA from RNA mixture was developed by using a biotinylated-PNA (peptide nucleic acid) that is complementary to the RNA. Efficient bindings of Escherichia coli tRNA(Leu)CUC were observed to the 9-mer and 12-mer PNAs that are complementary to the 3'-end region of the tRNA. Purified E. coli tRNA(Leu)CUC was obtained from E. coli tRNA mixture.

Original language	English
Pages (from-to)	263-264
Number of pages	2
Journal	Nucleic acids symposium series (2004)
Issue number	49
DOIs	https://doi.org/10.1093/nass/49.1.263
Publication status	Published - 2005

ASJC Scopus subject areas

Medicine(all)

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Ohtsuki, T., Kumano, C., Manabe, T., Kitamatsu, M., & Sisido, M. (2005). RNA isolation using immobilized PNA. Nucleic acids symposium series (2004), (49), 263-264. https://doi.org/10.1093/nass/49.1.263

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author = "Takashi Ohtsuki and Chisato Kumano and Taishi Manabe and Mizuki Kitamatsu and Masahiko Sisido",

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