RNA isolation using immobilized PNA.

Takashi Ohtsuki; Chisato Kumano; Taishi Manabe; Mizuki Kitamatsu; Masahiko Sisido

doi:10.1093/nass/49.1.263

RNA isolation using immobilized PNA.

Takashi Ohtsuki, Chisato Kumano, Taishi Manabe, Mizuki Kitamatsu, Masahiko Sisido

Research output: Contribution to journal › Article › peer-review

Abstract

A novel method to isolate a single kind of RNA from RNA mixture was developed by using a biotinylated-PNA (peptide nucleic acid) that is complementary to the RNA. Efficient bindings of Escherichia coli tRNA(Leu)CUC were observed to the 9-mer and 12-mer PNAs that are complementary to the 3'-end region of the tRNA. Purified E. coli tRNA(Leu)CUC was obtained from E. coli tRNA mixture.

Original language	English
Pages (from-to)	263-264
Number of pages	2
Journal	Nucleic acids symposium series (2004)
Issue number	49
DOIs	https://doi.org/10.1093/nass/49.1.263
Publication status	Published - 2005

ASJC Scopus subject areas

Medicine(all)

Access to Document

10.1093/nass/49.1.263

Fingerprint Dive into the research topics of 'RNA isolation using immobilized PNA.'. Together they form a unique fingerprint.

Cite this

@article{baaf458045cc44a48b7835cf5be3bdaf,

title = "RNA isolation using immobilized PNA.",

abstract = "A novel method to isolate a single kind of RNA from RNA mixture was developed by using a biotinylated-PNA (peptide nucleic acid) that is complementary to the RNA. Efficient bindings of Escherichia coli tRNA(Leu)CUC were observed to the 9-mer and 12-mer PNAs that are complementary to the 3'-end region of the tRNA. Purified E. coli tRNA(Leu)CUC was obtained from E. coli tRNA mixture.",

author = "Takashi Ohtsuki and Chisato Kumano and Taishi Manabe and Mizuki Kitamatsu and Masahiko Sisido",

note = "Copyright: This record is sourced from MEDLINE/PubMed, a database of the U.S. National Library of Medicine",

year = "2005",

doi = "10.1093/nass/49.1.263",

language = "English",

pages = "263--264",

journal = "Nucleic acids symposium series (2004)",

issn = "1746-8272",

publisher = "Oxford University Press",

number = "49",

}

TY - JOUR

T1 - RNA isolation using immobilized PNA.

AU - Ohtsuki, Takashi

AU - Kumano, Chisato

AU - Manabe, Taishi

AU - Kitamatsu, Mizuki

AU - Sisido, Masahiko

N1 - Copyright: This record is sourced from MEDLINE/PubMed, a database of the U.S. National Library of Medicine

PY - 2005

Y1 - 2005

N2 - A novel method to isolate a single kind of RNA from RNA mixture was developed by using a biotinylated-PNA (peptide nucleic acid) that is complementary to the RNA. Efficient bindings of Escherichia coli tRNA(Leu)CUC were observed to the 9-mer and 12-mer PNAs that are complementary to the 3'-end region of the tRNA. Purified E. coli tRNA(Leu)CUC was obtained from E. coli tRNA mixture.

AB - A novel method to isolate a single kind of RNA from RNA mixture was developed by using a biotinylated-PNA (peptide nucleic acid) that is complementary to the RNA. Efficient bindings of Escherichia coli tRNA(Leu)CUC were observed to the 9-mer and 12-mer PNAs that are complementary to the 3'-end region of the tRNA. Purified E. coli tRNA(Leu)CUC was obtained from E. coli tRNA mixture.

UR - http://www.scopus.com/inward/record.url?scp=39049184719&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=39049184719&partnerID=8YFLogxK

U2 - 10.1093/nass/49.1.263

DO - 10.1093/nass/49.1.263

M3 - Article

C2 - 17150734

AN - SCOPUS:39049184719

SP - 263

EP - 264

JO - Nucleic acids symposium series (2004)

JF - Nucleic acids symposium series (2004)

SN - 1746-8272

IS - 49

ER -

RNA isolation using immobilized PNA.

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Cite this