RNA isolation using immobilized PNA.

Takashi Ohtsuki; Chisato Kumano; Taishi Manabe; Mizuki Kitamatsu; Masahiko Sisido

doi:10.1093/nass/49.1.263

RNA isolation using immobilized PNA.

Takashi Ohtsuki, Chisato Kumano, Taishi Manabe, Mizuki Kitamatsu, Masahiko Sisido

Research output: Contribution to journal › Article › peer-review

Abstract

A novel method to isolate a single kind of RNA from RNA mixture was developed by using a biotinylated-PNA (peptide nucleic acid) that is complementary to the RNA. Efficient bindings of Escherichia coli tRNA(Leu)CUC were observed to the 9-mer and 12-mer PNAs that are complementary to the 3'-end region of the tRNA. Purified E. coli tRNA(Leu)CUC was obtained from E. coli tRNA mixture.

Original language	English
Pages (from-to)	263-264
Number of pages	2
Journal	Nucleic acids symposium series (2004)
Issue number	49
DOIs	https://doi.org/10.1093/nass/49.1.263
Publication status	Published - 2005

ASJC Scopus subject areas

Medicine(all)

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title = "RNA isolation using immobilized PNA.",

abstract = "A novel method to isolate a single kind of RNA from RNA mixture was developed by using a biotinylated-PNA (peptide nucleic acid) that is complementary to the RNA. Efficient bindings of Escherichia coli tRNA(Leu)CUC were observed to the 9-mer and 12-mer PNAs that are complementary to the 3'-end region of the tRNA. Purified E. coli tRNA(Leu)CUC was obtained from E. coli tRNA mixture.",

author = "Takashi Ohtsuki and Chisato Kumano and Taishi Manabe and Mizuki Kitamatsu and Masahiko Sisido",

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AU - Ohtsuki, Takashi

AU - Kumano, Chisato

AU - Manabe, Taishi

AU - Kitamatsu, Mizuki

AU - Sisido, Masahiko

N1 - Copyright: This record is sourced from MEDLINE/PubMed, a database of the U.S. National Library of Medicine

PY - 2005

Y1 - 2005

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