RNA helicase, CrhR is indispensable for the energy redistribution and the regulation of photosystem stoichiometry at low temperature in Synechocystis sp. PCC6803

Kodru Sireesha, Balaga Radharani, Pilla Sankara Krishna, Nellaepalli Sreedhar, Rajagopal Subramanyam, Prasanna Mohanty, Jogadhenu S.S. Prakash

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

We investigated the role of a cold-inducible and redox-regulated RNA helicase, CrhR, in the energy redistribution and adjustment of stoichiometry between photosystem I (PSI) and photosystem II (PSII), at low temperature in Synechocystis sp. PCC 6803. The results suggest that during low temperature incubation, i.e., when cells are shifted from 34 °C to 24 °C, wild type cells exhibited light-induced state transitions, whereas the mutant deficient in CrhR failed to perform the same. At low temperature, wild type cells maintained the plastoquinone (PQ) pool in the reduced state due to enhanced respiratory electron flow to the PQ pool, whereas in ΠcrhR mutant cells the PQ pool was in the oxidized state. Wild type cells were in state 2 and ΠcrhR cells were locked in state 1 at low temperature. In both wild type and ΠcrhR cells, a fraction of PSI trimers were changed to PSI monomers. However, in ΠcrhR cells, the PSI trimer content was significantly decreased. Expression of photosystem I genes, especially the psaA and psaB, was strongly down-regulated due to oxidation of downstream components of PQ in ΠcrhR cells at low temperature. We demonstrated that changes in the low temperature-induced energy redistribution and regulation of photosystem stoichiometry are acclimatization responses exerted by Synechocystis cells, essentially regulated by the RNA helicase, CrhR, at low temperature.

Original languageEnglish
Pages (from-to)1525-1536
Number of pages12
JournalBiochimica et Biophysica Acta - Bioenergetics
Volume1817
Issue number9
DOIs
Publication statusPublished - Sep 2012
Externally publishedYes

Keywords

  • Low temperature
  • Photosystem stoichiometry
  • Redox regulation
  • Synechocystis sp. PCC6803
  • ΠcrhR mutant

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Cell Biology

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