Abstract
Background: Rikkunshito (RKT) is a gastroprotective herbal medicine. In this study, we investigated the role of RKT in the relaxation of the gastric body (fundus and corpus) and antrum. Methods: We used Suncus murinus, a unique small model animal with similar gastrointestinal motility to humans and dogs. RKT was added at 0.1, 1.0, and 5.0 mg/mL to induce relaxation in vitro; the outcome measure was the intensity of relaxation. The number of spontaneous antral contractions in the absence or the presence of RKT was also counted. Key Results: Rikkunshito induced the relaxation of the gastric body and antrum and decreased the number of spontaneous antral contractions in a dose-dependent manner. The responses to RKT (1.0 mg/mL) were not affected by pretreatment with atropine, N-nitro-l-arginine methyl ester, ritanserin, or ondansetron. On the other hand, timolol almost completely reversed the relaxation induced by RKT (1.0 mg/mL) on the gastric body and antrum and the occurrence of the spontaneous antral contractions. Both butoxamine, a β2-adrenoreceptor antagonist, and L 748337, a β3-adrenoreceptor antagonist, but not CGP 20712, a β1-adrenoreceptor antagonist, significantly reversed the RKT-induced (1.0 mg/mL) gastric relaxation. Conclusions & Inferences: These results indicate that RKT stimulates and modulates gastric relaxation through β2- and β3-adrenergic, but not β1-adrenergic, pathways in S. murinus.
| Original language | English |
|---|---|
| Pages (from-to) | 875-884 |
| Number of pages | 10 |
| Journal | Neurogastroenterology and Motility |
| Volume | 27 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - Jun 1 2015 |
| Externally published | Yes |
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Keywords
- Rikkunshito
- Adrenergic receptor
- Gastric relaxation
ASJC Scopus subject areas
- Endocrine and Autonomic Systems
- Gastroenterology
- Physiology
Cite this
Rikkunshito induces gastric relaxation via the β-adrenergic pathway in Suncus murinus. / Mondal, A.; Takehara, A.; Aizawa, Sayaka; Tanaka, T.; Fujitsuka, N.; Hattori, T.; Sakai, Takafumi; Sakata, Ichiro.
In: Neurogastroenterology and Motility, Vol. 27, No. 6, 01.06.2015, p. 875-884.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Rikkunshito induces gastric relaxation via the β-adrenergic pathway in Suncus murinus
AU - Mondal, A.
AU - Takehara, A.
AU - Aizawa, Sayaka
AU - Tanaka, T.
AU - Fujitsuka, N.
AU - Hattori, T.
AU - Sakai, Takafumi
AU - Sakata, Ichiro
PY - 2015/6/1
Y1 - 2015/6/1
N2 - Background: Rikkunshito (RKT) is a gastroprotective herbal medicine. In this study, we investigated the role of RKT in the relaxation of the gastric body (fundus and corpus) and antrum. Methods: We used Suncus murinus, a unique small model animal with similar gastrointestinal motility to humans and dogs. RKT was added at 0.1, 1.0, and 5.0 mg/mL to induce relaxation in vitro; the outcome measure was the intensity of relaxation. The number of spontaneous antral contractions in the absence or the presence of RKT was also counted. Key Results: Rikkunshito induced the relaxation of the gastric body and antrum and decreased the number of spontaneous antral contractions in a dose-dependent manner. The responses to RKT (1.0 mg/mL) were not affected by pretreatment with atropine, N-nitro-l-arginine methyl ester, ritanserin, or ondansetron. On the other hand, timolol almost completely reversed the relaxation induced by RKT (1.0 mg/mL) on the gastric body and antrum and the occurrence of the spontaneous antral contractions. Both butoxamine, a β2-adrenoreceptor antagonist, and L 748337, a β3-adrenoreceptor antagonist, but not CGP 20712, a β1-adrenoreceptor antagonist, significantly reversed the RKT-induced (1.0 mg/mL) gastric relaxation. Conclusions & Inferences: These results indicate that RKT stimulates and modulates gastric relaxation through β2- and β3-adrenergic, but not β1-adrenergic, pathways in S. murinus.
AB - Background: Rikkunshito (RKT) is a gastroprotective herbal medicine. In this study, we investigated the role of RKT in the relaxation of the gastric body (fundus and corpus) and antrum. Methods: We used Suncus murinus, a unique small model animal with similar gastrointestinal motility to humans and dogs. RKT was added at 0.1, 1.0, and 5.0 mg/mL to induce relaxation in vitro; the outcome measure was the intensity of relaxation. The number of spontaneous antral contractions in the absence or the presence of RKT was also counted. Key Results: Rikkunshito induced the relaxation of the gastric body and antrum and decreased the number of spontaneous antral contractions in a dose-dependent manner. The responses to RKT (1.0 mg/mL) were not affected by pretreatment with atropine, N-nitro-l-arginine methyl ester, ritanserin, or ondansetron. On the other hand, timolol almost completely reversed the relaxation induced by RKT (1.0 mg/mL) on the gastric body and antrum and the occurrence of the spontaneous antral contractions. Both butoxamine, a β2-adrenoreceptor antagonist, and L 748337, a β3-adrenoreceptor antagonist, but not CGP 20712, a β1-adrenoreceptor antagonist, significantly reversed the RKT-induced (1.0 mg/mL) gastric relaxation. Conclusions & Inferences: These results indicate that RKT stimulates and modulates gastric relaxation through β2- and β3-adrenergic, but not β1-adrenergic, pathways in S. murinus.
KW - Rikkunshito
KW - Adrenergic receptor
KW - Gastric relaxation
UR - http://www.scopus.com/inward/record.url?scp=84929656075&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84929656075&partnerID=8YFLogxK
U2 - 10.1111/nmo.12564
DO - 10.1111/nmo.12564
M3 - Article
C2 - 25846270
AN - SCOPUS:84929656075
VL - 27
SP - 875
EP - 884
JO - Neurogastroenterology and Motility
JF - Neurogastroenterology and Motility
SN - 1350-1925
IS - 6
ER -