Rikkunshito induces gastric relaxation via the β-adrenergic pathway in Suncus murinus

A. Mondal, A. Takehara, S. Aizawa, T. Tanaka, N. Fujitsuka, T. Hattori, Takafumi Sakai, Ichiro Sakata

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2 Citations (Scopus)

Abstract

Background: Rikkunshito (RKT) is a gastroprotective herbal medicine. In this study, we investigated the role of RKT in the relaxation of the gastric body (fundus and corpus) and antrum. Methods: We used Suncus murinus, a unique small model animal with similar gastrointestinal motility to humans and dogs. RKT was added at 0.1, 1.0, and 5.0 mg/mL to induce relaxation in vitro; the outcome measure was the intensity of relaxation. The number of spontaneous antral contractions in the absence or the presence of RKT was also counted. Key Results: Rikkunshito induced the relaxation of the gastric body and antrum and decreased the number of spontaneous antral contractions in a dose-dependent manner. The responses to RKT (1.0 mg/mL) were not affected by pretreatment with atropine, N-nitro-l-arginine methyl ester, ritanserin, or ondansetron. On the other hand, timolol almost completely reversed the relaxation induced by RKT (1.0 mg/mL) on the gastric body and antrum and the occurrence of the spontaneous antral contractions. Both butoxamine, a β2-adrenoreceptor antagonist, and L 748337, a β3-adrenoreceptor antagonist, but not CGP 20712, a β1-adrenoreceptor antagonist, significantly reversed the RKT-induced (1.0 mg/mL) gastric relaxation. Conclusions & Inferences: These results indicate that RKT stimulates and modulates gastric relaxation through β2- and β3-adrenergic, but not β1-adrenergic, pathways in S. murinus.

Original languageEnglish
Pages (from-to)875-884
Number of pages10
JournalNeurogastroenterology and Motility
Volume27
Issue number6
DOIs
Publication statusPublished - Jun 1 2015
Externally publishedYes

Keywords

  • Adrenergic receptor
  • Gastric relaxation
  • Rikkunshito

ASJC Scopus subject areas

  • Physiology
  • Endocrine and Autonomic Systems
  • Gastroenterology

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  • Cite this

    Mondal, A., Takehara, A., Aizawa, S., Tanaka, T., Fujitsuka, N., Hattori, T., Sakai, T., & Sakata, I. (2015). Rikkunshito induces gastric relaxation via the β-adrenergic pathway in Suncus murinus. Neurogastroenterology and Motility, 27(6), 875-884. https://doi.org/10.1111/nmo.12564