TY - JOUR
T1 - Rice dwarf phytoreovirus segment S12 transcript is tricistronic in Vitro
AU - Suzuki, Nobuhiro
AU - Sugawara, Mikiko
AU - Kusano, Tomonobu
N1 - Funding Information:
The authors are grateful to Dr. Marilyn Kozakfor useful information on tricistronic mRNAs, Dr. Kripamoy Aguan for fruitful discussion, and to Ms. Kouko Futada for typing the manuscript. This work was partly supported by the Nissan Science Foundation.
PY - 1992/12
Y1 - 1992/12
N2 - Sequence analysis revealed that rice dwarf phytoreovirus segment S12 is 1066 nucleotides long with a small out-of-phase, overlapping open reading frame (ORF) as well as a major ORF. The large ORF (positions 42 to 980) encodes 312 amino acids, while the small one (bases 313 to 591) encodes 92 amino acids with an additional in-frame AUG codon (positions 337-339) 24 nucleotides downstream from the first one. Transcripts from a full-length cDNA directed the in vitro synthesis of three polypeptides of 33 (considered to be translated from the long ORF), 8, and 7 kDa. Alteration of each of the two ATG codons on the small ORF demonstrated their involvement in the generation of the 8- and 7-kDa polypeptides. Although it is still unknown whether these proteins are expressed in vivo, the small ORF is shown to be conserved in S9s of two other members of the genus Phytoreovirus, rice gall dwarf virus and wound tumor virus, suggesting its common, important function.
AB - Sequence analysis revealed that rice dwarf phytoreovirus segment S12 is 1066 nucleotides long with a small out-of-phase, overlapping open reading frame (ORF) as well as a major ORF. The large ORF (positions 42 to 980) encodes 312 amino acids, while the small one (bases 313 to 591) encodes 92 amino acids with an additional in-frame AUG codon (positions 337-339) 24 nucleotides downstream from the first one. Transcripts from a full-length cDNA directed the in vitro synthesis of three polypeptides of 33 (considered to be translated from the long ORF), 8, and 7 kDa. Alteration of each of the two ATG codons on the small ORF demonstrated their involvement in the generation of the 8- and 7-kDa polypeptides. Although it is still unknown whether these proteins are expressed in vivo, the small ORF is shown to be conserved in S9s of two other members of the genus Phytoreovirus, rice gall dwarf virus and wound tumor virus, suggesting its common, important function.
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U2 - 10.1016/0042-6822(92)90279-X
DO - 10.1016/0042-6822(92)90279-X
M3 - Article
C2 - 1448932
AN - SCOPUS:0027104832
SN - 0042-6822
VL - 191
SP - 992
EP - 995
JO - Virology
JF - Virology
IS - 2
ER -