Rice CYO1, an ortholog of Arabidopsis thaliana cotyledon chloroplast biogenesis factor AtCYO1, is expressed in leaves and involved in photosynthetic performance

Jun Tominaga, Haruka Mizutani, Daisuke Horikawa, Yasutoshi Nakahara, Tsuneaki Takami, Wataru Sakamoto, Atsushi Sakamoto, Hiroshi Shimada

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

In the dicotyledonous plant Arabidopsis thaliana, the cotyledon chloroplast biogenesis factor AtCYO1 is crucial for the biogenesis of cotyledon chloroplasts. Arabidopsis mutants lacking AtCYO1 have pale cotyledons but develop normal mature leaves. In the monocotyledonous plant Oryza sativa, the gene OsCYO1 has high sequence identity to AtCYO1, but its function is unknown. We examined the role of OsCYO1 in O. sativa. We first confirmed that transformation with OsCYO1 could recover the phenotype of the Arabidopsis cyo1 mutant. Similar to AtCYO1, recombinant OsCYO1 has protein disulfide reductase (PDR) activity, which increased as a function of dieosin glutathione disulfide concentration with an apparent Km of 3.2 μM and Kcat of 0.53 min−1. The PDR activity was reduced when NADPH or NADH was used as an electron donor; however, PDR activity was observed with OsCYO1 and glutathione, suggesting that glutathione may serve as a reducing agent for OsCYO1 in vivo. In O. sativa, the OsCYO1 transcript level was higher in leaves compared with the coleoptile, which is the first leaf-like organ that forms during rice embryogenesis. Many OsCYO1 mutant lines defective in RNA interference had green leaves, however, three mutant lines had not only albino coleoptile but also albino leaves. Those having green leaves reduced photosynthetic performance in leaves. Our results demonstrate that OsCYO1 is enzymatically equivalent to AtCYO1 but that the physiological role of OsCYO1 in monocotyledonous plants may differ from that of AtCYO1 in dicotyledonous plants.

Original languageEnglish
Pages (from-to)78-83
Number of pages6
JournalJournal of Plant Physiology
Volume207
DOIs
Publication statusPublished - Dec 1 2016

Fingerprint

Cotyledon
Chloroplasts
Arabidopsis
cotyledons
Arabidopsis thaliana
chloroplasts
rice
Disulfides
sulfides
Oxidoreductases
leaves
glutathione
mutants
Oryza sativa
Glutathione
coleoptiles
Magnoliopsida
albino
Proteins
Reducing Agents

Keywords

  • Coleoptile
  • Cotyledon
  • CYO1
  • Leaf
  • Oryza sativa
  • Pulse amplitude modulation

ASJC Scopus subject areas

  • Physiology
  • Agronomy and Crop Science
  • Plant Science

Cite this

Rice CYO1, an ortholog of Arabidopsis thaliana cotyledon chloroplast biogenesis factor AtCYO1, is expressed in leaves and involved in photosynthetic performance. / Tominaga, Jun; Mizutani, Haruka; Horikawa, Daisuke; Nakahara, Yasutoshi; Takami, Tsuneaki; Sakamoto, Wataru; Sakamoto, Atsushi; Shimada, Hiroshi.

In: Journal of Plant Physiology, Vol. 207, 01.12.2016, p. 78-83.

Research output: Contribution to journalArticle

Tominaga, Jun ; Mizutani, Haruka ; Horikawa, Daisuke ; Nakahara, Yasutoshi ; Takami, Tsuneaki ; Sakamoto, Wataru ; Sakamoto, Atsushi ; Shimada, Hiroshi. / Rice CYO1, an ortholog of Arabidopsis thaliana cotyledon chloroplast biogenesis factor AtCYO1, is expressed in leaves and involved in photosynthetic performance. In: Journal of Plant Physiology. 2016 ; Vol. 207. pp. 78-83.
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AU - Tominaga, Jun

AU - Mizutani, Haruka

AU - Horikawa, Daisuke

AU - Nakahara, Yasutoshi

AU - Takami, Tsuneaki

AU - Sakamoto, Wataru

AU - Sakamoto, Atsushi

AU - Shimada, Hiroshi

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