Rice α-fucosidase active against plant complex type N-glycans containing Lewis a epitope: Purification and characterization

Md Ziaur Rahman, Makoto Fujishige, Megumi Maeda, Yoshinobu Kimura

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Rice α-fucosidase (α-fucosidase Os, 58 kDa) that is active for α1-4 fucosyl linkage in Lewis a unit of plant N-glycans was purified to homogeneity. α-fucosidase Os showed activity against α1-3 fucosyl linkage in Lacto-N-fucopentaose III but not α1-3 fucosyl linkage in the core of plant N-glycans. The N-terminal sequence of α-fucosidase Os was identified as A-A-PT- P-P-P-L-, and this sequence was found in the amino acid sequence of the putative rice α-fucosidase 1 (Os04g0560400).

Original languageEnglish
Pages (from-to)291-294
Number of pages4
JournalBioscience, Biotechnology and Biochemistry
Volume80
Issue number2
DOIs
Publication statusPublished - 2016

Fingerprint

alpha-L-Fucosidase
Purification
Polysaccharides
Epitopes
Amino Acid Sequence
Amino Acids
Oryza

Keywords

  • N-glycan degradation
  • Oryza sativa
  • Plant N-glycan
  • α-fucosidase

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Applied Microbiology and Biotechnology
  • Analytical Chemistry
  • Organic Chemistry

Cite this

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abstract = "Rice α-fucosidase (α-fucosidase Os, 58 kDa) that is active for α1-4 fucosyl linkage in Lewis a unit of plant N-glycans was purified to homogeneity. α-fucosidase Os showed activity against α1-3 fucosyl linkage in Lacto-N-fucopentaose III but not α1-3 fucosyl linkage in the core of plant N-glycans. The N-terminal sequence of α-fucosidase Os was identified as A-A-PT- P-P-P-L-, and this sequence was found in the amino acid sequence of the putative rice α-fucosidase 1 (Os04g0560400).",
keywords = "N-glycan degradation, Oryza sativa, Plant N-glycan, α-fucosidase",
author = "Rahman, {Md Ziaur} and Makoto Fujishige and Megumi Maeda and Yoshinobu Kimura",
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language = "English",
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T1 - Rice α-fucosidase active against plant complex type N-glycans containing Lewis a epitope

T2 - Purification and characterization

AU - Rahman, Md Ziaur

AU - Fujishige, Makoto

AU - Maeda, Megumi

AU - Kimura, Yoshinobu

PY - 2016

Y1 - 2016

N2 - Rice α-fucosidase (α-fucosidase Os, 58 kDa) that is active for α1-4 fucosyl linkage in Lewis a unit of plant N-glycans was purified to homogeneity. α-fucosidase Os showed activity against α1-3 fucosyl linkage in Lacto-N-fucopentaose III but not α1-3 fucosyl linkage in the core of plant N-glycans. The N-terminal sequence of α-fucosidase Os was identified as A-A-PT- P-P-P-L-, and this sequence was found in the amino acid sequence of the putative rice α-fucosidase 1 (Os04g0560400).

AB - Rice α-fucosidase (α-fucosidase Os, 58 kDa) that is active for α1-4 fucosyl linkage in Lewis a unit of plant N-glycans was purified to homogeneity. α-fucosidase Os showed activity against α1-3 fucosyl linkage in Lacto-N-fucopentaose III but not α1-3 fucosyl linkage in the core of plant N-glycans. The N-terminal sequence of α-fucosidase Os was identified as A-A-PT- P-P-P-L-, and this sequence was found in the amino acid sequence of the putative rice α-fucosidase 1 (Os04g0560400).

KW - N-glycan degradation

KW - Oryza sativa

KW - Plant N-glycan

KW - α-fucosidase

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