Ribosome rescue systems in bacteria

Hyouta Himeno, Nobukazu Nameki, Daisuke Kurita, Akira Muto, Tatsuhiko Abo

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Abstract Ribosomes often stall during protein synthesis in various situations in a cell, either unexpectedly or in a programmed fashion. While some of them remain stalled for gene regulation, many are rescued by some cellular systems. Ribosomes stalled at the 3′ end of a truncated mRNA lacking a stop codon (non-stop mRNA) are rescued by trans-translation mediated by tmRNA (transfer-messenger RNA) and a partner protein, SmpB. Through trans-translation, a degradation tag is added to the C-termini of truncated polypeptides from a truncated mRNA to prevent them from accumulation in the cell. Trans-translation has crucial roles in a wide variety of cellular events, especially under stressful conditions. The trans-translation system is thought to be universally present in the bacterial domain, although it is not necessarily essential in all bacterial cells. It has recently been revealed that two other systems, one involving a small protein, ArfA, with RF2 and the other involving YaeJ (ArfB), a class I release factor homologue, operate to relieve ribosome stalling in Escherichia coli. Thus, many bacterial species would have multiple systems to cope with various kinds of stalled translation events.

Original languageEnglish
Article number4598
Pages (from-to)102-112
Number of pages11
JournalBiochimie
Volume114
DOIs
Publication statusPublished - Aug 31 2015

Fingerprint

Ribosomes
Bacteria
Messenger RNA
Terminator Codon
Transfer RNA
Gene expression
Escherichia coli
Proteins
Degradation
Peptides
Genes

Keywords

  • ArfA
  • Ribosome rescue
  • SmpB
  • tmRNA
  • YaeJ

ASJC Scopus subject areas

  • Biochemistry

Cite this

Himeno, H., Nameki, N., Kurita, D., Muto, A., & Abo, T. (2015). Ribosome rescue systems in bacteria. Biochimie, 114, 102-112. [4598]. https://doi.org/10.1016/j.biochi.2014.11.014

Ribosome rescue systems in bacteria. / Himeno, Hyouta; Nameki, Nobukazu; Kurita, Daisuke; Muto, Akira; Abo, Tatsuhiko.

In: Biochimie, Vol. 114, 4598, 31.08.2015, p. 102-112.

Research output: Contribution to journalArticle

Himeno, H, Nameki, N, Kurita, D, Muto, A & Abo, T 2015, 'Ribosome rescue systems in bacteria', Biochimie, vol. 114, 4598, pp. 102-112. https://doi.org/10.1016/j.biochi.2014.11.014
Himeno H, Nameki N, Kurita D, Muto A, Abo T. Ribosome rescue systems in bacteria. Biochimie. 2015 Aug 31;114:102-112. 4598. https://doi.org/10.1016/j.biochi.2014.11.014
Himeno, Hyouta ; Nameki, Nobukazu ; Kurita, Daisuke ; Muto, Akira ; Abo, Tatsuhiko. / Ribosome rescue systems in bacteria. In: Biochimie. 2015 ; Vol. 114. pp. 102-112.
@article{3b7b33c7c4d94bd8b86c400765e1cd8d,
title = "Ribosome rescue systems in bacteria",
abstract = "Abstract Ribosomes often stall during protein synthesis in various situations in a cell, either unexpectedly or in a programmed fashion. While some of them remain stalled for gene regulation, many are rescued by some cellular systems. Ribosomes stalled at the 3′ end of a truncated mRNA lacking a stop codon (non-stop mRNA) are rescued by trans-translation mediated by tmRNA (transfer-messenger RNA) and a partner protein, SmpB. Through trans-translation, a degradation tag is added to the C-termini of truncated polypeptides from a truncated mRNA to prevent them from accumulation in the cell. Trans-translation has crucial roles in a wide variety of cellular events, especially under stressful conditions. The trans-translation system is thought to be universally present in the bacterial domain, although it is not necessarily essential in all bacterial cells. It has recently been revealed that two other systems, one involving a small protein, ArfA, with RF2 and the other involving YaeJ (ArfB), a class I release factor homologue, operate to relieve ribosome stalling in Escherichia coli. Thus, many bacterial species would have multiple systems to cope with various kinds of stalled translation events.",
keywords = "ArfA, Ribosome rescue, SmpB, tmRNA, YaeJ",
author = "Hyouta Himeno and Nobukazu Nameki and Daisuke Kurita and Akira Muto and Tatsuhiko Abo",
year = "2015",
month = "8",
day = "31",
doi = "10.1016/j.biochi.2014.11.014",
language = "English",
volume = "114",
pages = "102--112",
journal = "Biochimie",
issn = "0300-9084",
publisher = "Elsevier",

}

TY - JOUR

T1 - Ribosome rescue systems in bacteria

AU - Himeno, Hyouta

AU - Nameki, Nobukazu

AU - Kurita, Daisuke

AU - Muto, Akira

AU - Abo, Tatsuhiko

PY - 2015/8/31

Y1 - 2015/8/31

N2 - Abstract Ribosomes often stall during protein synthesis in various situations in a cell, either unexpectedly or in a programmed fashion. While some of them remain stalled for gene regulation, many are rescued by some cellular systems. Ribosomes stalled at the 3′ end of a truncated mRNA lacking a stop codon (non-stop mRNA) are rescued by trans-translation mediated by tmRNA (transfer-messenger RNA) and a partner protein, SmpB. Through trans-translation, a degradation tag is added to the C-termini of truncated polypeptides from a truncated mRNA to prevent them from accumulation in the cell. Trans-translation has crucial roles in a wide variety of cellular events, especially under stressful conditions. The trans-translation system is thought to be universally present in the bacterial domain, although it is not necessarily essential in all bacterial cells. It has recently been revealed that two other systems, one involving a small protein, ArfA, with RF2 and the other involving YaeJ (ArfB), a class I release factor homologue, operate to relieve ribosome stalling in Escherichia coli. Thus, many bacterial species would have multiple systems to cope with various kinds of stalled translation events.

AB - Abstract Ribosomes often stall during protein synthesis in various situations in a cell, either unexpectedly or in a programmed fashion. While some of them remain stalled for gene regulation, many are rescued by some cellular systems. Ribosomes stalled at the 3′ end of a truncated mRNA lacking a stop codon (non-stop mRNA) are rescued by trans-translation mediated by tmRNA (transfer-messenger RNA) and a partner protein, SmpB. Through trans-translation, a degradation tag is added to the C-termini of truncated polypeptides from a truncated mRNA to prevent them from accumulation in the cell. Trans-translation has crucial roles in a wide variety of cellular events, especially under stressful conditions. The trans-translation system is thought to be universally present in the bacterial domain, although it is not necessarily essential in all bacterial cells. It has recently been revealed that two other systems, one involving a small protein, ArfA, with RF2 and the other involving YaeJ (ArfB), a class I release factor homologue, operate to relieve ribosome stalling in Escherichia coli. Thus, many bacterial species would have multiple systems to cope with various kinds of stalled translation events.

KW - ArfA

KW - Ribosome rescue

KW - SmpB

KW - tmRNA

KW - YaeJ

UR - http://www.scopus.com/inward/record.url?scp=84930382700&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84930382700&partnerID=8YFLogxK

U2 - 10.1016/j.biochi.2014.11.014

DO - 10.1016/j.biochi.2014.11.014

M3 - Article

C2 - 25446863

AN - SCOPUS:84930382700

VL - 114

SP - 102

EP - 112

JO - Biochimie

JF - Biochimie

SN - 0300-9084

M1 - 4598

ER -