Ribosome rescue activity of an arabidopsis thaliana ArfB homolog

Michiaki Nagao, Fumina Tsuchiya, Reiko Motohashi, Tatsuhiko Abo

Research output: Contribution to journalArticlepeer-review

Abstract

A homolog of the bacterial ribosome rescue factor ArfB was identified in Arabidopsis thaliana. The factor, named AtArfB for Arabidopsis thaliana ArfB, showed ribosome rescue activity in both in vivo and in vitro assays based on the bacterial translation system. As has been shown for ArfB, the ribosome rescue activity of AtArfB was dependent on the GGQ motif, the crucial motif for the function of class I release factors and ArfB. The C-terminal region of AtArfB was also important for its function. The N-terminal region of AtArfB, which is absent in bacterial ArfB, functioned as a transit peptide for chloroplast targeting in tobacco cells. These results strongly suggest that AtArfB is a ribosome rescue factor that functions in chloroplasts.

Original languageEnglish
Pages (from-to)119-131
Number of pages13
JournalGenes and Genetic Systems
Volume95
Issue number3
DOIs
Publication statusPublished - 2020

Keywords

  • Arabidopsis thaliana
  • ArfB
  • Chloroplast
  • Ribosome rescue
  • Translation

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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