Rhodococcus fascians: Shoot proliferation without elevated cytokinins?

Ivan Galis, Kristin Bilyeu, Geraldine Wood, Paula E. Jameson

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

In order to determine whether the disease symptoms caused by virulent strains of Rhodococcus fascians are due to increased cytokinin activity in infected tissues, germinating peas (Pisum sativum cv Novella) were inoculated with either a virulent strain or a nonvirulent strain of Rhodococcus fascians. The nonvirulent strain lacked both the ipt gene and the putative cytokinin oxidase/dehydrogenase homologue, fas5. Control peas were not inoculated. Twelve cytokinins were isolated from pea shoots 3, 6 and 9 days post-inoculation. Within 6 days of inoculation the levels of cytokinin free bases, ribosides, O-glucosides and nucleotides were decreased in shoots inoculated with the virulent strain, and were increased in shoots inoculated with the nonvirulent strain relative to the uninoculated control. The results are discussed with respect to the classic Skoog and Miller (1965) model of organogenesis and to the possible involvement of the plant cytokinin oxidase/dehydrogenase during infection by virulent strains of R. fascians.

Original languageEnglish
Pages (from-to)109-115
Number of pages7
JournalPlant Growth Regulation
Volume46
Issue number2
DOIs
Publication statusPublished - Jun 1 2005
Externally publishedYes

    Fingerprint

Keywords

  • Cytokinin
  • Cytokinin oxidase/dehydrogenase
  • Cytokinin synthase
  • Plant pathogen interactions
  • Rhodococcus fascians
  • ipt

ASJC Scopus subject areas

  • Physiology
  • Agronomy and Crop Science
  • Plant Science

Cite this