Rho-ROCK signal pathway regulates microtubule-based process formation of cultured podocytes - Inhibition of ROCK promoted process elongation

Shuang Yan Gao, Chun Yu Li, Jie Chen, Lei Pan, Shouichiro Saito, Takehiro Terashita, Kyoko Saito, Kyojy Miyawaki, Kazuhiro Shigemoto, Katsumi Mominoki, Seiji Matsuda, Naoto Kobayashi

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Background: Podocytes, renal glomerular visceral epithelial cells, have two kinds of processes, namely major processes containing microtubules (MTs) and foot processes with actin filaments (AFs). The present study investigated how MTs are organized by the Rho-ROCK signal transduction pathway during process formation of podocytes. Method: After induction of differentiation, podocytes of the conditionally immortalized mouse cell line were treated with Y-27632, a specific inhibitor of ROCK, and exoenzyme C3, an inhibitor of RhoA, as well as with forskolin whose effects include inhibition of RhoA, in order to inhibit the Rho-ROCK pathway. Results: Inhibition of ROCK significantly enhanced the formation of thick processes containing MT bundles. Y-27632 promoted process formation even in the presence of latrunculin A which disrupts AFs, strongly suggesting that ROCK directly regulates MT assembly. Treatment with Y-27632 increased MT stability, and stabilized MTs preferentially localized in podocyte processes. Moreover, when treated with a combination of Y-27632 and forskolin, and with Y-27632 and C3 as well, podocytes developed not only MT-based thick processes but also AF-based thin projections. Conclusions: These data indicate a contribution of ROCK in MT organization to promote podocyte process formation, although it was originally thought to regulate AF assembly. AF-based thin projections seem to be induced mainly by inhibition of RhoA and ROCK. The present study reveals a significant role of the Rho-ROCK signal pathway in the reorganization of both MTs and AFs during process formation of podocytes.

Original languageEnglish
JournalNephron - Experimental Nephrology
Volume97
Issue number2
DOIs
Publication statusPublished - 2004
Externally publishedYes

Fingerprint

Podocytes
Microtubules
Signal Transduction
Actin Cytoskeleton
Colforsin
Foot
Y 27632
Kidney
Cell Line

Keywords

  • Cytoskeleton
  • Microtubule
  • Podocyte
  • Process formation
  • Renal glomerulus
  • Rho
  • ROCK
  • Y-27632

ASJC Scopus subject areas

  • Nephrology

Cite this

Rho-ROCK signal pathway regulates microtubule-based process formation of cultured podocytes - Inhibition of ROCK promoted process elongation. / Gao, Shuang Yan; Li, Chun Yu; Chen, Jie; Pan, Lei; Saito, Shouichiro; Terashita, Takehiro; Saito, Kyoko; Miyawaki, Kyojy; Shigemoto, Kazuhiro; Mominoki, Katsumi; Matsuda, Seiji; Kobayashi, Naoto.

In: Nephron - Experimental Nephrology, Vol. 97, No. 2, 2004.

Research output: Contribution to journalArticle

Gao, SY, Li, CY, Chen, J, Pan, L, Saito, S, Terashita, T, Saito, K, Miyawaki, K, Shigemoto, K, Mominoki, K, Matsuda, S & Kobayashi, N 2004, 'Rho-ROCK signal pathway regulates microtubule-based process formation of cultured podocytes - Inhibition of ROCK promoted process elongation', Nephron - Experimental Nephrology, vol. 97, no. 2. https://doi.org/10.1159/000078406
Gao, Shuang Yan ; Li, Chun Yu ; Chen, Jie ; Pan, Lei ; Saito, Shouichiro ; Terashita, Takehiro ; Saito, Kyoko ; Miyawaki, Kyojy ; Shigemoto, Kazuhiro ; Mominoki, Katsumi ; Matsuda, Seiji ; Kobayashi, Naoto. / Rho-ROCK signal pathway regulates microtubule-based process formation of cultured podocytes - Inhibition of ROCK promoted process elongation. In: Nephron - Experimental Nephrology. 2004 ; Vol. 97, No. 2.
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T1 - Rho-ROCK signal pathway regulates microtubule-based process formation of cultured podocytes - Inhibition of ROCK promoted process elongation

AU - Gao, Shuang Yan

AU - Li, Chun Yu

AU - Chen, Jie

AU - Pan, Lei

AU - Saito, Shouichiro

AU - Terashita, Takehiro

AU - Saito, Kyoko

AU - Miyawaki, Kyojy

AU - Shigemoto, Kazuhiro

AU - Mominoki, Katsumi

AU - Matsuda, Seiji

AU - Kobayashi, Naoto

PY - 2004

Y1 - 2004

N2 - Background: Podocytes, renal glomerular visceral epithelial cells, have two kinds of processes, namely major processes containing microtubules (MTs) and foot processes with actin filaments (AFs). The present study investigated how MTs are organized by the Rho-ROCK signal transduction pathway during process formation of podocytes. Method: After induction of differentiation, podocytes of the conditionally immortalized mouse cell line were treated with Y-27632, a specific inhibitor of ROCK, and exoenzyme C3, an inhibitor of RhoA, as well as with forskolin whose effects include inhibition of RhoA, in order to inhibit the Rho-ROCK pathway. Results: Inhibition of ROCK significantly enhanced the formation of thick processes containing MT bundles. Y-27632 promoted process formation even in the presence of latrunculin A which disrupts AFs, strongly suggesting that ROCK directly regulates MT assembly. Treatment with Y-27632 increased MT stability, and stabilized MTs preferentially localized in podocyte processes. Moreover, when treated with a combination of Y-27632 and forskolin, and with Y-27632 and C3 as well, podocytes developed not only MT-based thick processes but also AF-based thin projections. Conclusions: These data indicate a contribution of ROCK in MT organization to promote podocyte process formation, although it was originally thought to regulate AF assembly. AF-based thin projections seem to be induced mainly by inhibition of RhoA and ROCK. The present study reveals a significant role of the Rho-ROCK signal pathway in the reorganization of both MTs and AFs during process formation of podocytes.

AB - Background: Podocytes, renal glomerular visceral epithelial cells, have two kinds of processes, namely major processes containing microtubules (MTs) and foot processes with actin filaments (AFs). The present study investigated how MTs are organized by the Rho-ROCK signal transduction pathway during process formation of podocytes. Method: After induction of differentiation, podocytes of the conditionally immortalized mouse cell line were treated with Y-27632, a specific inhibitor of ROCK, and exoenzyme C3, an inhibitor of RhoA, as well as with forskolin whose effects include inhibition of RhoA, in order to inhibit the Rho-ROCK pathway. Results: Inhibition of ROCK significantly enhanced the formation of thick processes containing MT bundles. Y-27632 promoted process formation even in the presence of latrunculin A which disrupts AFs, strongly suggesting that ROCK directly regulates MT assembly. Treatment with Y-27632 increased MT stability, and stabilized MTs preferentially localized in podocyte processes. Moreover, when treated with a combination of Y-27632 and forskolin, and with Y-27632 and C3 as well, podocytes developed not only MT-based thick processes but also AF-based thin projections. Conclusions: These data indicate a contribution of ROCK in MT organization to promote podocyte process formation, although it was originally thought to regulate AF assembly. AF-based thin projections seem to be induced mainly by inhibition of RhoA and ROCK. The present study reveals a significant role of the Rho-ROCK signal pathway in the reorganization of both MTs and AFs during process formation of podocytes.

KW - Cytoskeleton

KW - Microtubule

KW - Podocyte

KW - Process formation

KW - Renal glomerulus

KW - Rho

KW - ROCK

KW - Y-27632

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