Rho-kinase regulates extracellular matrix-mediated osteogenic differentiation of periodontal ligament cells

Yuki Ugawa, Tadashi Yamamoto, Mari Kawamura, Keisuke Yamashiro, Masayuki Shimoe, Kazuya Tomikawa, Shoichi Hongo, Hiroshi Maeda, Shogo Takashiba

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The periodontal ligament (PDL) cells contain heterogeneous mesenchymal cell populations, which have the ability to differentiate into cells that produce adjacent mineralized tissues and abundant extracellular matrix (ECM). ECM is essential not only for the homeostasis of the periodontal tissue, but also for controlling the differentiation of the PDL cells. The process of differentiation involves mechanotransduction, which links the ECM to the cytoskeleton. The present study investigated the roles of Rho-associated coiled-coil containing protein kinase (ROCK) signaling, a crucial regulator of the cytoskeleton, during ECM-mediated osteogenic differentiation of PDL cells in vitro. The PDL cells were isolated from human periodontal ligaments of extracted teeth and cultured in osteogenic medium with or without Y-27632, a pharmacological inhibitor of ROCK. ECM-coated plates were used for ECM-mediated differentiation. The osteogenic phenotype was evaluated at different time points by real-time RT-PCR for the gene encoding alkaline phosphatase (ALP) and an ALP activity assay. The effects of ROCK on cytoskeletal changes and ECM synthesis were examined by immunofluorescence analysis. Y-27632 significantly inhibited ALP at the mRNA and protein activity levels in the late stage of differentiation; concomitantly, the actin filament content and the extracellular levels of collagen-I and fibronectin were markedly decreased by Y-27632. Exogenous collagen-I and fibronectin temporally increased ALP activity, with fibronectin showing a more pronounced effect. Importantly, ECM-mediated differentiation was almost completely inhibited by Y-27632. These findings indicated that ECM-mediated differentiation is dependent on ROCK signaling, and ROCK signaling contributes to the establishment of the ECM microenvironment for PDL cell differentiation.

Original languageEnglish
Pages (from-to)651-658
Number of pages8
JournalCell Biology International
Volume41
Issue number6
DOIs
Publication statusPublished - Jun 1 2017

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rho-Associated Kinases
Periodontal Ligament
Extracellular Matrix
Alkaline Phosphatase
Fibronectins
Cytoskeleton
Collagen
Actin Cytoskeleton
Fluorescent Antibody Technique
Real-Time Polymerase Chain Reaction
Cell Differentiation
Tooth
Homeostasis
Pharmacology
Phenotype

Keywords

  • collagen
  • differentiation
  • fibronectin
  • periodontal ligament cells
  • Rho signaling

ASJC Scopus subject areas

  • Cell Biology

Cite this

Rho-kinase regulates extracellular matrix-mediated osteogenic differentiation of periodontal ligament cells. / Ugawa, Yuki; Yamamoto, Tadashi; Kawamura, Mari; Yamashiro, Keisuke; Shimoe, Masayuki; Tomikawa, Kazuya; Hongo, Shoichi; Maeda, Hiroshi; Takashiba, Shogo.

In: Cell Biology International, Vol. 41, No. 6, 01.06.2017, p. 651-658.

Research output: Contribution to journalArticle

Ugawa, Yuki ; Yamamoto, Tadashi ; Kawamura, Mari ; Yamashiro, Keisuke ; Shimoe, Masayuki ; Tomikawa, Kazuya ; Hongo, Shoichi ; Maeda, Hiroshi ; Takashiba, Shogo. / Rho-kinase regulates extracellular matrix-mediated osteogenic differentiation of periodontal ligament cells. In: Cell Biology International. 2017 ; Vol. 41, No. 6. pp. 651-658.
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