RFLP analysis of nuclear DNAs homologous with mitochondrial plasmid-like DNAs in cultivated rice

W. Sakamoto, K. Kadowaki, N. Kishimoto, M. Yano, A. Saito, S. Tano

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

B1 and B2 are small, circular, mitochondrial plasmid-like DNAs found in male-sterile cytoplasm (cms-Bo) of rice. In this study, nuclear sequences homologous to these DNAs were investigated among a number of rice cultivars. Several copies of nuclear B1-and B2-homologous sequences were detected in all examined cultivars, regardless of the presence or absence of the B1 and B2 DNAs in mitochondria, indicating that the existence of the B1- and B2-homologous sequences in the rice nuclear genome was widespread. A restriction fragment length polymorphism (RFLP) was detected for both sequences, and we propose that these DNAs could be useful RFLP markers for the rice nuclear genome. To analyze these nuclear homologues genetically, segregation analysis of the RFLP was carried out in the F2 progenies of an Indica-Japonica rice hybrid. Of the B1 homologues, there were two nonallelic fragments, one specific to the Indica parent and the other to the Japonica. These results indicate that the B1 and B2 homologues were dispersed in the nuclear genome. The integration of B1-homologous DNA into the nuclear DNA may have occurred independently after sexual isolation of the Indica and Japonica rice varietal groups, or a intranuclear transposition of these sequences took place during the process of rice differentiation into the varietal groups.

Original languageEnglish
Pages (from-to)179-184
Number of pages6
JournalTheoretical and Applied Genetics
Volume82
Issue number2
DOIs
Publication statusPublished - Aug 1991
Externally publishedYes

Keywords

  • Mitochondrial DNA
  • Nucleo-mitochondrial DNA transmission
  • Plasmid-like DNA
  • RFLP
  • Rice

ASJC Scopus subject areas

  • Biotechnology
  • Agronomy and Crop Science
  • Genetics

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