Reverse genetic identification of CRN1 and its distinctive role in chlorophyll degradation in arabidopsis

Guodong Ren, Qian Zhou, Shouxin Wu, Yufan Zhang, Lingang Zhang, Jirong Huang, Zhenfei Sun, Benke Kuai

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Recent identification of NYE1/SGR1 brought up a new era for the exploration of the regulatory mechanism of Chlorophyll (Chl) degradation. Cluster analysis of senescence associated genes with putative chloroplast targeting sequences revealed several genes sharing a similar expression pattern with NYE1. Further characterization of available T-DNA insertion lines led to the discovery of a novel stay-green gene CRN1 (Co-regulated with NYE1). Chl breakdown was significantly restrained in crn1-1 under diversified senescence scenarios, which is comparable with that in acd1-20, but much more severe than that in nye1-1. Notably, various Chl binding proteins, especially trimeric LHCP II, were markedly retained in crn1-1 four days after dark-treatment, possibly due to a lesion in disassociation of protein-pigment complex. Nevertheless, the photochemical efficiency of PSII in crn1-1 declined, even more rapidly, two days after dark-treatment, compared to those in Col-0 and nye1-1. Our results suggest that CRN1 plays a crucial role in Chl degradation, and that loss of its function produces various side-effects, including those on the breakdown of Ch-protein complex and the maintenance of the residual photosynthetic capability during leaf senescence.

Original languageEnglish
Pages (from-to)496-504
Number of pages9
JournalJournal of Integrative Plant Biology
Volume52
Issue number5
DOIs
Publication statusPublished - May 2010
Externally publishedYes

Fingerprint

Reverse Genetics
Chlorophyll
Arabidopsis
Genes
chlorophyll
Degradation
degradation
Chlorophyll Binding Proteins
lesions (plant)
genes
Cluster analysis
Chloroplasts
Pigments
Cluster Analysis
cluster analysis
Proteins
proteins
chloroplasts
adverse effects
pigments

ASJC Scopus subject areas

  • Plant Science
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Medicine(all)

Cite this

Reverse genetic identification of CRN1 and its distinctive role in chlorophyll degradation in arabidopsis. / Ren, Guodong; Zhou, Qian; Wu, Shouxin; Zhang, Yufan; Zhang, Lingang; Huang, Jirong; Sun, Zhenfei; Kuai, Benke.

In: Journal of Integrative Plant Biology, Vol. 52, No. 5, 05.2010, p. 496-504.

Research output: Contribution to journalArticle

Ren, Guodong ; Zhou, Qian ; Wu, Shouxin ; Zhang, Yufan ; Zhang, Lingang ; Huang, Jirong ; Sun, Zhenfei ; Kuai, Benke. / Reverse genetic identification of CRN1 and its distinctive role in chlorophyll degradation in arabidopsis. In: Journal of Integrative Plant Biology. 2010 ; Vol. 52, No. 5. pp. 496-504.
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