TY - JOUR
T1 - Retinal stem/progenitor properties of iris pigment epithelial cells
AU - Sun, Guangwei
AU - Asami, Maki
AU - Ohta, Hiroshi
AU - Kosaka, Jun
AU - Kosaka, Mitsuko
N1 - Funding Information:
The authors thank Dr. Shichida (Kyoto University, Japan) for providing the iodopsin antibody and Dr. Araki (Nara Women's University, Japan) for providing useful information regarding retinal specific antibodies. The authors also thank the staffs in the Department of Cytology and Histology in Okayama University, Japan, for their helpful supports. This work is supported by the Japan Science and Technology Agency (to M.K.) and by the Leading Project, the Ministry of Education, Culture, Sports, Science and Technology, Japan (to M.K.). A Japan Society for the Promotion of Science Postdoctoral Fellowship (to GW.S.) also supported this work.
PY - 2006/1/1
Y1 - 2006/1/1
N2 - Neural stem cells/progenitors that give rise to neurons and glia have been identified in different regions of the brain, including the embryonic retina and ciliary epithelium of the adult eye. Here, we first demonstrate the characterization of neural stem/progenitors in postnatal iris pigment epithelial (IPE) cells. Pure isolated IPE cells could form spheres that contained cells expressing retinal progenitor markers in non-adherent culture. The spheres grew by cell proliferation, as indicated by bromodeoxyuridine incorporation. When attached to laminin, the spheres forming IPE derived cells were able to exhibit neural phenotypes, including retinal-specific neurons. When co-cultured with embryonic retinal cells, or grafted into embryonic retina in vivo, the IPE cells could also display the phenotypes of photoreceptor neurons and Muller glia. Our results suggest that the IPE derived cells have retinal stem/progenitor properties and neurogenic potential without gene transfer, thereby providing a novel potential source for both basic stem cell biology and therapeutic applications for retinal diseases.
AB - Neural stem cells/progenitors that give rise to neurons and glia have been identified in different regions of the brain, including the embryonic retina and ciliary epithelium of the adult eye. Here, we first demonstrate the characterization of neural stem/progenitors in postnatal iris pigment epithelial (IPE) cells. Pure isolated IPE cells could form spheres that contained cells expressing retinal progenitor markers in non-adherent culture. The spheres grew by cell proliferation, as indicated by bromodeoxyuridine incorporation. When attached to laminin, the spheres forming IPE derived cells were able to exhibit neural phenotypes, including retinal-specific neurons. When co-cultured with embryonic retinal cells, or grafted into embryonic retina in vivo, the IPE cells could also display the phenotypes of photoreceptor neurons and Muller glia. Our results suggest that the IPE derived cells have retinal stem/progenitor properties and neurogenic potential without gene transfer, thereby providing a novel potential source for both basic stem cell biology and therapeutic applications for retinal diseases.
KW - IPE
KW - Regeneration
KW - Retinal neuron
KW - Retinal stem/progenitor
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U2 - 10.1016/j.ydbio.2005.10.035
DO - 10.1016/j.ydbio.2005.10.035
M3 - Article
C2 - 16310762
AN - SCOPUS:29144481935
VL - 289
SP - 243
EP - 252
JO - Developmental Biology
JF - Developmental Biology
SN - 0012-1606
IS - 1
ER -