Retinal stem/progenitor properties of iris pigment epithelial cells

Guangwei Sun, Maki Asami, Hiroshi Ohta, Jun Kosaka, Mitsuko Kosaka

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

Neural stem cells/progenitors that give rise to neurons and glia have been identified in different regions of the brain, including the embryonic retina and ciliary epithelium of the adult eye. Here, we first demonstrate the characterization of neural stem/progenitors in postnatal iris pigment epithelial (IPE) cells. Pure isolated IPE cells could form spheres that contained cells expressing retinal progenitor markers in non-adherent culture. The spheres grew by cell proliferation, as indicated by bromodeoxyuridine incorporation. When attached to laminin, the spheres forming IPE derived cells were able to exhibit neural phenotypes, including retinal-specific neurons. When co-cultured with embryonic retinal cells, or grafted into embryonic retina in vivo, the IPE cells could also display the phenotypes of photoreceptor neurons and Muller glia. Our results suggest that the IPE derived cells have retinal stem/progenitor properties and neurogenic potential without gene transfer, thereby providing a novel potential source for both basic stem cell biology and therapeutic applications for retinal diseases.

Original languageEnglish
Pages (from-to)243-252
Number of pages10
JournalDevelopmental Biology
Volume289
Issue number1
DOIs
Publication statusPublished - Jan 1 2006
Externally publishedYes

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Keywords

  • IPE
  • Regeneration
  • Retinal neuron
  • Retinal stem/progenitor

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

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