Abstract
Neural stem cells/progenitors that give rise to neurons and glia have been identified in different regions of the brain, including the embryonic retina and ciliary epithelium of the adult eye. Here, we first demonstrate the characterization of neural stem/progenitors in postnatal iris pigment epithelial (IPE) cells. Pure isolated IPE cells could form spheres that contained cells expressing retinal progenitor markers in non-adherent culture. The spheres grew by cell proliferation, as indicated by bromodeoxyuridine incorporation. When attached to laminin, the spheres forming IPE derived cells were able to exhibit neural phenotypes, including retinal-specific neurons. When co-cultured with embryonic retinal cells, or grafted into embryonic retina in vivo, the IPE cells could also display the phenotypes of photoreceptor neurons and Muller glia. Our results suggest that the IPE derived cells have retinal stem/progenitor properties and neurogenic potential without gene transfer, thereby providing a novel potential source for both basic stem cell biology and therapeutic applications for retinal diseases.
| Original language | English |
|---|---|
| Pages (from-to) | 243-252 |
| Number of pages | 10 |
| Journal | Developmental Biology |
| Volume | 289 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Jan 1 2006 |
| Externally published | Yes |
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Keywords
- IPE
- Regeneration
- Retinal neuron
- Retinal stem/progenitor
ASJC Scopus subject areas
- Developmental Biology
Cite this
Retinal stem/progenitor properties of iris pigment epithelial cells. / Sun, Guangwei; Asami, Maki; Ohta, Hiroshi; Kosaka, Jun; Kosaka, Mitsuko.
In: Developmental Biology, Vol. 289, No. 1, 01.01.2006, p. 243-252.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Retinal stem/progenitor properties of iris pigment epithelial cells
AU - Sun, Guangwei
AU - Asami, Maki
AU - Ohta, Hiroshi
AU - Kosaka, Jun
AU - Kosaka, Mitsuko
PY - 2006/1/1
Y1 - 2006/1/1
N2 - Neural stem cells/progenitors that give rise to neurons and glia have been identified in different regions of the brain, including the embryonic retina and ciliary epithelium of the adult eye. Here, we first demonstrate the characterization of neural stem/progenitors in postnatal iris pigment epithelial (IPE) cells. Pure isolated IPE cells could form spheres that contained cells expressing retinal progenitor markers in non-adherent culture. The spheres grew by cell proliferation, as indicated by bromodeoxyuridine incorporation. When attached to laminin, the spheres forming IPE derived cells were able to exhibit neural phenotypes, including retinal-specific neurons. When co-cultured with embryonic retinal cells, or grafted into embryonic retina in vivo, the IPE cells could also display the phenotypes of photoreceptor neurons and Muller glia. Our results suggest that the IPE derived cells have retinal stem/progenitor properties and neurogenic potential without gene transfer, thereby providing a novel potential source for both basic stem cell biology and therapeutic applications for retinal diseases.
AB - Neural stem cells/progenitors that give rise to neurons and glia have been identified in different regions of the brain, including the embryonic retina and ciliary epithelium of the adult eye. Here, we first demonstrate the characterization of neural stem/progenitors in postnatal iris pigment epithelial (IPE) cells. Pure isolated IPE cells could form spheres that contained cells expressing retinal progenitor markers in non-adherent culture. The spheres grew by cell proliferation, as indicated by bromodeoxyuridine incorporation. When attached to laminin, the spheres forming IPE derived cells were able to exhibit neural phenotypes, including retinal-specific neurons. When co-cultured with embryonic retinal cells, or grafted into embryonic retina in vivo, the IPE cells could also display the phenotypes of photoreceptor neurons and Muller glia. Our results suggest that the IPE derived cells have retinal stem/progenitor properties and neurogenic potential without gene transfer, thereby providing a novel potential source for both basic stem cell biology and therapeutic applications for retinal diseases.
KW - IPE
KW - Regeneration
KW - Retinal neuron
KW - Retinal stem/progenitor
UR - http://www.scopus.com/inward/record.url?scp=29144481935&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=29144481935&partnerID=8YFLogxK
U2 - 10.1016/j.ydbio.2005.10.035
DO - 10.1016/j.ydbio.2005.10.035
M3 - Article
C2 - 16310762
AN - SCOPUS:29144481935
VL - 289
SP - 243
EP - 252
JO - Developmental Biology
JF - Developmental Biology
SN - 0012-1606
IS - 1
ER -