Reticalmin: A novel calcium-calmodulin-dependent protein kinase IV-like protein in rat retina

Kazuyuki Hirooka, Masaaki Tokuda, Toyohiro Tsumura, Bushra Y. Ahmed, Toshifumi Itano, Hideki Matsui, Ryoji Konishi, Eiichi Hasegawa, Sachiko Okuno, Takako Kitani, Hitoshi Fujisawa, Osamu Hatase

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Western blot analysis of 100,000g supernatant of rat retina using a polyclonal anti-Ca2+/calmodulin-dependent protein kinase IV (CaM-kinase IV) antibody revealed an immunoreactive mass of 35 kDa, termed reticalmin. Lower amount of a isoform of CaM-kinase IV was also expressed in rat retina. Reticalmin did not react with anti-CaM-kinase IV C-terminal peptide antibody which recognized α and β isoforms of CaM-kinase IV and calspermin. Immunohistochemically reticalmin was shown to be localized mainly in the outer segment of photo-receptor cells, and in dendrites of inner plexiform layers and may be in nuclei of ganglion cells and some inner nuclear layer cells.

Original languageEnglish
Pages (from-to)2029-2033
Number of pages5
JournalVision Research
Volume37
Issue number15
DOIs
Publication statusPublished - Aug 1997

Fingerprint

Calcium-Calmodulin-Dependent Protein Kinase Type 4
Retina
Protein Isoforms
Proteins
Antibodies
Dendrites
Cell Nucleus
Ganglia
Protein Kinase C
Western Blotting
Peptides
reticalmin

Keywords

  • Ca-calmodulin-dependent protein kinase IV
  • Reticalmin
  • Retina

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems

Cite this

Hirooka, K., Tokuda, M., Tsumura, T., Ahmed, B. Y., Itano, T., Matsui, H., ... Hatase, O. (1997). Reticalmin: A novel calcium-calmodulin-dependent protein kinase IV-like protein in rat retina. Vision Research, 37(15), 2029-2033. https://doi.org/10.1016/S0042-6989(97)00175-2

Reticalmin : A novel calcium-calmodulin-dependent protein kinase IV-like protein in rat retina. / Hirooka, Kazuyuki; Tokuda, Masaaki; Tsumura, Toyohiro; Ahmed, Bushra Y.; Itano, Toshifumi; Matsui, Hideki; Konishi, Ryoji; Hasegawa, Eiichi; Okuno, Sachiko; Kitani, Takako; Fujisawa, Hitoshi; Hatase, Osamu.

In: Vision Research, Vol. 37, No. 15, 08.1997, p. 2029-2033.

Research output: Contribution to journalArticle

Hirooka, K, Tokuda, M, Tsumura, T, Ahmed, BY, Itano, T, Matsui, H, Konishi, R, Hasegawa, E, Okuno, S, Kitani, T, Fujisawa, H & Hatase, O 1997, 'Reticalmin: A novel calcium-calmodulin-dependent protein kinase IV-like protein in rat retina', Vision Research, vol. 37, no. 15, pp. 2029-2033. https://doi.org/10.1016/S0042-6989(97)00175-2
Hirooka, Kazuyuki ; Tokuda, Masaaki ; Tsumura, Toyohiro ; Ahmed, Bushra Y. ; Itano, Toshifumi ; Matsui, Hideki ; Konishi, Ryoji ; Hasegawa, Eiichi ; Okuno, Sachiko ; Kitani, Takako ; Fujisawa, Hitoshi ; Hatase, Osamu. / Reticalmin : A novel calcium-calmodulin-dependent protein kinase IV-like protein in rat retina. In: Vision Research. 1997 ; Vol. 37, No. 15. pp. 2029-2033.
@article{d163b5c005154e1899202a51587e04da,
title = "Reticalmin: A novel calcium-calmodulin-dependent protein kinase IV-like protein in rat retina",
abstract = "Western blot analysis of 100,000g supernatant of rat retina using a polyclonal anti-Ca2+/calmodulin-dependent protein kinase IV (CaM-kinase IV) antibody revealed an immunoreactive mass of 35 kDa, termed reticalmin. Lower amount of a isoform of CaM-kinase IV was also expressed in rat retina. Reticalmin did not react with anti-CaM-kinase IV C-terminal peptide antibody which recognized α and β isoforms of CaM-kinase IV and calspermin. Immunohistochemically reticalmin was shown to be localized mainly in the outer segment of photo-receptor cells, and in dendrites of inner plexiform layers and may be in nuclei of ganglion cells and some inner nuclear layer cells.",
keywords = "Ca-calmodulin-dependent protein kinase IV, Reticalmin, Retina",
author = "Kazuyuki Hirooka and Masaaki Tokuda and Toyohiro Tsumura and Ahmed, {Bushra Y.} and Toshifumi Itano and Hideki Matsui and Ryoji Konishi and Eiichi Hasegawa and Sachiko Okuno and Takako Kitani and Hitoshi Fujisawa and Osamu Hatase",
year = "1997",
month = "8",
doi = "10.1016/S0042-6989(97)00175-2",
language = "English",
volume = "37",
pages = "2029--2033",
journal = "Vision Research",
issn = "0042-6989",
publisher = "Elsevier Limited",
number = "15",

}

TY - JOUR

T1 - Reticalmin

T2 - A novel calcium-calmodulin-dependent protein kinase IV-like protein in rat retina

AU - Hirooka, Kazuyuki

AU - Tokuda, Masaaki

AU - Tsumura, Toyohiro

AU - Ahmed, Bushra Y.

AU - Itano, Toshifumi

AU - Matsui, Hideki

AU - Konishi, Ryoji

AU - Hasegawa, Eiichi

AU - Okuno, Sachiko

AU - Kitani, Takako

AU - Fujisawa, Hitoshi

AU - Hatase, Osamu

PY - 1997/8

Y1 - 1997/8

N2 - Western blot analysis of 100,000g supernatant of rat retina using a polyclonal anti-Ca2+/calmodulin-dependent protein kinase IV (CaM-kinase IV) antibody revealed an immunoreactive mass of 35 kDa, termed reticalmin. Lower amount of a isoform of CaM-kinase IV was also expressed in rat retina. Reticalmin did not react with anti-CaM-kinase IV C-terminal peptide antibody which recognized α and β isoforms of CaM-kinase IV and calspermin. Immunohistochemically reticalmin was shown to be localized mainly in the outer segment of photo-receptor cells, and in dendrites of inner plexiform layers and may be in nuclei of ganglion cells and some inner nuclear layer cells.

AB - Western blot analysis of 100,000g supernatant of rat retina using a polyclonal anti-Ca2+/calmodulin-dependent protein kinase IV (CaM-kinase IV) antibody revealed an immunoreactive mass of 35 kDa, termed reticalmin. Lower amount of a isoform of CaM-kinase IV was also expressed in rat retina. Reticalmin did not react with anti-CaM-kinase IV C-terminal peptide antibody which recognized α and β isoforms of CaM-kinase IV and calspermin. Immunohistochemically reticalmin was shown to be localized mainly in the outer segment of photo-receptor cells, and in dendrites of inner plexiform layers and may be in nuclei of ganglion cells and some inner nuclear layer cells.

KW - Ca-calmodulin-dependent protein kinase IV

KW - Reticalmin

KW - Retina

UR - http://www.scopus.com/inward/record.url?scp=0030845476&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030845476&partnerID=8YFLogxK

U2 - 10.1016/S0042-6989(97)00175-2

DO - 10.1016/S0042-6989(97)00175-2

M3 - Article

C2 - 9327050

AN - SCOPUS:0030845476

VL - 37

SP - 2029

EP - 2033

JO - Vision Research

JF - Vision Research

SN - 0042-6989

IS - 15

ER -