Abstract
Hydroquinone (1,4-benzenediol) has been widely used in clinical situations and the cosmetic industry because of its depigmenting effects. Most skin-lightening hydroquinone creams contain 4%-5% hydroquinone. We have investigated the role of resveratrol in prevention of hydroquinone induced cytotoxicity in mouse primary hepatocytes. We found that 400 μM hydroquinone exposure alone induced apoptosis of the cells and also resulted in a significant drop of cell viability compared with the control, and pretreatment of resveratrol to a final concentration of 0.5 mM 1 h before hydroquinone exposure did not show a significant improvement in the survival rate of the hepatocytes, however, relatively higher concentrations of resveratrol (≥1 mM) inhibited apoptosis of the mouse primary hepatocytes and increased cell viability in a dose-dependent manner, and in particular the survival rate of the hepatocytes was recovered from 28% to near 100% by 5 mM resveratrol. Interestingly, pretreatment with resveratrol for longer time (24 h), even in very low concentrations (50 μM, 100 μM), blocked the damage of hydroquinone to the cells. We also observed that resveratrol pretreatment suppressed hydroquinone-induced expression of cytochrome P450 2E1 mRNA dose-dependently. The present study suggests that resveratrol protected the cells against hydroquinone-induced toxicity through its antioxidant function and possibly suppressive effect on the expression of cytochrome P450 2E1.
| Original language | English |
|---|---|
| Pages (from-to) | 3354-3364 |
| Number of pages | 11 |
| Journal | International Journal of Environmental Research and Public Health |
| Volume | 9 |
| Issue number | 9 |
| DOIs | |
| Publication status | Published - Sep 2012 |
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Keywords
- CYP 2E1
- Cytotoxicity prevention
- Hydroquinone
- Primary culture of mouse hepatocytes
- Resveratrol
ASJC Scopus subject areas
- Public Health, Environmental and Occupational Health
- Health, Toxicology and Mutagenesis
Cite this
Resveratrol inhibited hydroquinone-induced cytotoxicity in mouse primary hepatocytes. / Wang, Da Hong; Ootsuki, Yoshie; Fujita, Hirofumi; Miyazaki, Masahiro; Yie, Qinxia; Tsutsui, Ken; Sano, Kuniaki; Masuoka, Noriyoshi; Ogino, Keiki.
In: International Journal of Environmental Research and Public Health, Vol. 9, No. 9, 09.2012, p. 3354-3364.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Resveratrol inhibited hydroquinone-induced cytotoxicity in mouse primary hepatocytes
AU - Wang, Da Hong
AU - Ootsuki, Yoshie
AU - Fujita, Hirofumi
AU - Miyazaki, Masahiro
AU - Yie, Qinxia
AU - Tsutsui, Ken
AU - Sano, Kuniaki
AU - Masuoka, Noriyoshi
AU - Ogino, Keiki
PY - 2012/9
Y1 - 2012/9
N2 - Hydroquinone (1,4-benzenediol) has been widely used in clinical situations and the cosmetic industry because of its depigmenting effects. Most skin-lightening hydroquinone creams contain 4%-5% hydroquinone. We have investigated the role of resveratrol in prevention of hydroquinone induced cytotoxicity in mouse primary hepatocytes. We found that 400 μM hydroquinone exposure alone induced apoptosis of the cells and also resulted in a significant drop of cell viability compared with the control, and pretreatment of resveratrol to a final concentration of 0.5 mM 1 h before hydroquinone exposure did not show a significant improvement in the survival rate of the hepatocytes, however, relatively higher concentrations of resveratrol (≥1 mM) inhibited apoptosis of the mouse primary hepatocytes and increased cell viability in a dose-dependent manner, and in particular the survival rate of the hepatocytes was recovered from 28% to near 100% by 5 mM resveratrol. Interestingly, pretreatment with resveratrol for longer time (24 h), even in very low concentrations (50 μM, 100 μM), blocked the damage of hydroquinone to the cells. We also observed that resveratrol pretreatment suppressed hydroquinone-induced expression of cytochrome P450 2E1 mRNA dose-dependently. The present study suggests that resveratrol protected the cells against hydroquinone-induced toxicity through its antioxidant function and possibly suppressive effect on the expression of cytochrome P450 2E1.
AB - Hydroquinone (1,4-benzenediol) has been widely used in clinical situations and the cosmetic industry because of its depigmenting effects. Most skin-lightening hydroquinone creams contain 4%-5% hydroquinone. We have investigated the role of resveratrol in prevention of hydroquinone induced cytotoxicity in mouse primary hepatocytes. We found that 400 μM hydroquinone exposure alone induced apoptosis of the cells and also resulted in a significant drop of cell viability compared with the control, and pretreatment of resveratrol to a final concentration of 0.5 mM 1 h before hydroquinone exposure did not show a significant improvement in the survival rate of the hepatocytes, however, relatively higher concentrations of resveratrol (≥1 mM) inhibited apoptosis of the mouse primary hepatocytes and increased cell viability in a dose-dependent manner, and in particular the survival rate of the hepatocytes was recovered from 28% to near 100% by 5 mM resveratrol. Interestingly, pretreatment with resveratrol for longer time (24 h), even in very low concentrations (50 μM, 100 μM), blocked the damage of hydroquinone to the cells. We also observed that resveratrol pretreatment suppressed hydroquinone-induced expression of cytochrome P450 2E1 mRNA dose-dependently. The present study suggests that resveratrol protected the cells against hydroquinone-induced toxicity through its antioxidant function and possibly suppressive effect on the expression of cytochrome P450 2E1.
KW - CYP 2E1
KW - Cytotoxicity prevention
KW - Hydroquinone
KW - Primary culture of mouse hepatocytes
KW - Resveratrol
UR - http://www.scopus.com/inward/record.url?scp=84866910152&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84866910152&partnerID=8YFLogxK
U2 - 10.3390/ijerph9093354
DO - 10.3390/ijerph9093354
M3 - Article
C2 - 23202692
AN - SCOPUS:84866910152
VL - 9
SP - 3354
EP - 3364
JO - International Journal of Environmental Research and Public Health
JF - International Journal of Environmental Research and Public Health
SN - 1661-7827
IS - 9
ER -