Restriction of protein synthesis abolishes senescence features at cellular and organismal levels

Yuki Takauji, Takumi Wada, Asuka Takeda, Ikuru Kudo, Kensuke Miki, Michihiko Fujii, Dai Ayusawa

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Cellular senescence or its equivalence is induced by treatment of cells with an appropriate inducer of senescence in various cell types. Mild restriction of cytoplasmic protein synthesis prevented induction of all aspects of cellular senescence in normal and tumor-derived human cells. It allowed the cells to continuously grow with no sign of senescent features in the presence of various inducers. It also delayed replicative senescence in normal human fibroblasts. Moreover, it allowed for growth of the cells that had entered a senescent state. When adult worms of the nematode C. elegans were grown under protein-restricted conditions, their average and maximal lifespans were significantly extended. These results suggest that accumulation of cytoplasmic proteins due to imbalance in macromolecule synthesis is a fundamental cause of cellular senescence.

Original languageEnglish
Article number18722
JournalScientific Reports
Publication statusPublished - Jan 5 2016
Externally publishedYes


ASJC Scopus subject areas

  • General

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