Restriction fragments homologous to mitochondrial plasmid-like DNAs are located within limited chromosomal regions on the rice nuclear genome

A. Kanazawa, N. Kishimoto, Wataru Sakamoto, R. Ohsawa, Y. Ukai, N. Tsutsumi, A. Hirai, A. Saito

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The chromosomal locations of restriction fragments of nuclear DNA that were homologous to four mitochondrial plasmid-like DNAs, namely, B1, B2, B3 and B4, were analyzed by restriction fragment length polymorphism (RFLP) analysis in cultivated rice. Nine kinds of fragments homologous to plasmidlike DNAs were analyzed for their segregation in three different F2 populations derived from intercrosses between rice subspecies; these were found to be localized in three chromosomal regions: three, one and five kinds of nuclear homologues were situated on chromosomes 1,3 and 8, respectively. Nuclear homologues on a given chromosome were tightly linked even though they were homologous to different plasmid-like DNAs. The loci of nuclear homologues found commonly in two or three cultivars were found to be highly conserved, a result that is consistent with their stable transmission. These results and those of the Southern analysis suggest the independent integration of these sequences during the varietal differentiation of rice. The concentration of loci for nuclear homologues on chromosomes 1 and 8 suggests that these sequences were integrated non-randomly into these chromosomal regions.

Original languageEnglish
Pages (from-to)577-586
Number of pages10
JournalTheoretical And Applied Genetics
Volume87
Issue number5
DOIs
Publication statusPublished - 1993

Fingerprint

nuclear genome
plasmids
Plasmids
Genome
chromosomes
rice
Chromosomes, Human, Pair 8
Chromosomes, Human, Pair 1
DNA
loci
Chromosomes, Human, Pair 3
restriction fragment length polymorphism
Restriction Fragment Length Polymorphisms
Chromosomes
cultivars
Oryza
Population

Keywords

  • Inter-organellar sequence transfer
  • Mitochondrial DNA
  • Nuclear homologue
  • Plasmid-like DNA
  • Rice

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Biotechnology
  • Genetics
  • Horticulture
  • Plant Science
  • Genetics(clinical)

Cite this

Restriction fragments homologous to mitochondrial plasmid-like DNAs are located within limited chromosomal regions on the rice nuclear genome. / Kanazawa, A.; Kishimoto, N.; Sakamoto, Wataru; Ohsawa, R.; Ukai, Y.; Tsutsumi, N.; Hirai, A.; Saito, A.

In: Theoretical And Applied Genetics, Vol. 87, No. 5, 1993, p. 577-586.

Research output: Contribution to journalArticle

Kanazawa, A. ; Kishimoto, N. ; Sakamoto, Wataru ; Ohsawa, R. ; Ukai, Y. ; Tsutsumi, N. ; Hirai, A. ; Saito, A. / Restriction fragments homologous to mitochondrial plasmid-like DNAs are located within limited chromosomal regions on the rice nuclear genome. In: Theoretical And Applied Genetics. 1993 ; Vol. 87, No. 5. pp. 577-586.
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AU - Kanazawa, A.

AU - Kishimoto, N.

AU - Sakamoto, Wataru

AU - Ohsawa, R.

AU - Ukai, Y.

AU - Tsutsumi, N.

AU - Hirai, A.

AU - Saito, A.

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N2 - The chromosomal locations of restriction fragments of nuclear DNA that were homologous to four mitochondrial plasmid-like DNAs, namely, B1, B2, B3 and B4, were analyzed by restriction fragment length polymorphism (RFLP) analysis in cultivated rice. Nine kinds of fragments homologous to plasmidlike DNAs were analyzed for their segregation in three different F2 populations derived from intercrosses between rice subspecies; these were found to be localized in three chromosomal regions: three, one and five kinds of nuclear homologues were situated on chromosomes 1,3 and 8, respectively. Nuclear homologues on a given chromosome were tightly linked even though they were homologous to different plasmid-like DNAs. The loci of nuclear homologues found commonly in two or three cultivars were found to be highly conserved, a result that is consistent with their stable transmission. These results and those of the Southern analysis suggest the independent integration of these sequences during the varietal differentiation of rice. The concentration of loci for nuclear homologues on chromosomes 1 and 8 suggests that these sequences were integrated non-randomly into these chromosomal regions.

AB - The chromosomal locations of restriction fragments of nuclear DNA that were homologous to four mitochondrial plasmid-like DNAs, namely, B1, B2, B3 and B4, were analyzed by restriction fragment length polymorphism (RFLP) analysis in cultivated rice. Nine kinds of fragments homologous to plasmidlike DNAs were analyzed for their segregation in three different F2 populations derived from intercrosses between rice subspecies; these were found to be localized in three chromosomal regions: three, one and five kinds of nuclear homologues were situated on chromosomes 1,3 and 8, respectively. Nuclear homologues on a given chromosome were tightly linked even though they were homologous to different plasmid-like DNAs. The loci of nuclear homologues found commonly in two or three cultivars were found to be highly conserved, a result that is consistent with their stable transmission. These results and those of the Southern analysis suggest the independent integration of these sequences during the varietal differentiation of rice. The concentration of loci for nuclear homologues on chromosomes 1 and 8 suggests that these sequences were integrated non-randomly into these chromosomal regions.

KW - Inter-organellar sequence transfer

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KW - Plasmid-like DNA

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