Restriction fragment length polymorphisms detected in N-ras-related sequences of rats and their linkage analyses

Tetsuo Kunieda, Eiji Kobayashi, Hiroshi Ikadai, Tomonori Imamichi, Nobuo Nomura, Ryotaro Ishizaki

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Novel restriction fragment length polymorphisms (RFLPs) in inbred rats were revealed with the human N-ras gene as probe. Three fragments hybridizing to the probe were detected by Southern blot hybridization under highly stringent conditions, and one of the fragments showed variation in inbred rat strains. Furthermore, on hybridization under low-stringency conditions, an additional fragment hybridizing to the probe was observed, and this fragment also showed interstrain variation. These two variant fragments showed different distributions in 27 inbred rat strains and segregated in backcross progeny as codominant alleles of independent single autosomal loci. Therefore, the loci for these RFLPs were named Nras-1 and Nras-2, respectively. Analyses of linkages between the RFLPs and 11 other loci revealed that the Nras-2 locus was closely linked to the c locus (3.7±2.6%), which belongs to rat linkage group I.

Original languageEnglish
Pages (from-to)631-642
Number of pages12
JournalBiochemical Genetics
Volume28
Issue number11-12
DOIs
Publication statusPublished - Dec 1990
Externally publishedYes

Fingerprint

Polymorphism
Restriction Fragment Length Polymorphisms
Inbred Strains Rats
linkage (genetics)
Rats
restriction fragment length polymorphism
polymorphism
probe
loci
rats
ras Genes
Southern Blotting
hybridization
allele
Alleles
linkage groups
gene
Genes
Southern blotting
alleles

Keywords

  • mammalian genetics
  • N-ras gene
  • Rattus norvegicus
  • restriction fragment length polymorphism (RFLP)

ASJC Scopus subject areas

  • Genetics
  • Biochemistry

Cite this

Restriction fragment length polymorphisms detected in N-ras-related sequences of rats and their linkage analyses. / Kunieda, Tetsuo; Kobayashi, Eiji; Ikadai, Hiroshi; Imamichi, Tomonori; Nomura, Nobuo; Ishizaki, Ryotaro.

In: Biochemical Genetics, Vol. 28, No. 11-12, 12.1990, p. 631-642.

Research output: Contribution to journalArticle

Kunieda, Tetsuo ; Kobayashi, Eiji ; Ikadai, Hiroshi ; Imamichi, Tomonori ; Nomura, Nobuo ; Ishizaki, Ryotaro. / Restriction fragment length polymorphisms detected in N-ras-related sequences of rats and their linkage analyses. In: Biochemical Genetics. 1990 ; Vol. 28, No. 11-12. pp. 631-642.
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