Restriction endonuclease AfaI from Acidiphilium facilis, a new isoschizomer of RsaI: purification and properties

Dexian Dou; Kenji Inagaki; Keiko Kita; Atsushi Ohshima; Nobutugu Hiraoka; Noriaki Kishimoto; Tsuyoshi Sugio; Tatsuo Tano

doi:10.1016/0167-4781(89)90082-1

Restriction endonuclease AfaI from Acidiphilium facilis, a new isoschizomer of RsaI: purification and properties

Dexian Dou, Kenji Inagaki, Keiko Kita, Atsushi Ohshima, Nobutugu Hiraoka, Noriaki Kishimoto, Tsuyoshi Sugio, Tatsuo Tano

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

Abstract

We have purified AfaI endonuclease, an isoschizomer of RsaI, from Acidiphilium facilis strain 28H. The enzyme is homogeneous as judged by polyacrylamide gel electrophoresis, and composed of a single polypeptide chain with a molecular weight of 30 000. AfaI endonuclease, like RsaI, recognizes the tetranucleotide sequence 5′-G-T-A-C-3′, and cleaves between the T and A to produce blunt-ended fragments. The yield of the enzyme is 50-100-times that of the RsaI, which is form a phototrophic bacterium, Rhodospseudomonas sphaeroides strain 28/5.

Original language	English
Pages (from-to)	83-86
Number of pages	4
Journal	BBA - Gene Structure and Expression
Volume	1009
Issue number	1
DOIs	https://doi.org/10.1016/0167-4781(89)90082-1
Publication status	Published - Sep 21 1989

Keywords

(A. facilis)
Acidophilic bacterium
Enzyme purification
Isoschizomer
Restriction endonuclease

ASJC Scopus subject areas

Structural Biology
Biophysics
Biochemistry
Genetics

Access to Document

10.1016/0167-4781(89)90082-1

Fingerprint Dive into the research topics of 'Restriction endonuclease AfaI from Acidiphilium facilis, a new isoschizomer of RsaI: purification and properties'. Together they form a unique fingerprint.

Cite this

Restriction endonuclease AfaI from Acidiphilium facilis, a new isoschizomer of RsaI : purification and properties. / Dou, Dexian; Inagaki, Kenji; Kita, Keiko; Ohshima, Atsushi; Hiraoka, Nobutugu; Kishimoto, Noriaki; Sugio, Tsuyoshi; Tano, Tatsuo.

In: BBA - Gene Structure and Expression, Vol. 1009, No. 1, 21.09.1989, p. 83-86.

Research output: Contribution to journal › Article › peer-review

@article{e59860f367da4dd39936881db664b180,

title = "Restriction endonuclease AfaI from Acidiphilium facilis, a new isoschizomer of RsaI: purification and properties",

abstract = "We have purified AfaI endonuclease, an isoschizomer of RsaI, from Acidiphilium facilis strain 28H. The enzyme is homogeneous as judged by polyacrylamide gel electrophoresis, and composed of a single polypeptide chain with a molecular weight of 30 000. AfaI endonuclease, like RsaI, recognizes the tetranucleotide sequence 5′-G-T-A-C-3′, and cleaves between the T and A to produce blunt-ended fragments. The yield of the enzyme is 50-100-times that of the RsaI, which is form a phototrophic bacterium, Rhodospseudomonas sphaeroides strain 28/5.",

keywords = "(A. facilis), Acidophilic bacterium, Enzyme purification, Isoschizomer, Restriction endonuclease",

author = "Dexian Dou and Kenji Inagaki and Keiko Kita and Atsushi Ohshima and Nobutugu Hiraoka and Noriaki Kishimoto and Tsuyoshi Sugio and Tatsuo Tano",

year = "1989",

month = sep,

day = "21",

doi = "10.1016/0167-4781(89)90082-1",

language = "English",

volume = "1009",

pages = "83--86",

journal = "Biochimica et Biophysica Acta - Gene Structure and Expression",

issn = "0167-4781",

publisher = "Elsevier BV",

number = "1",

}

TY - JOUR

T1 - Restriction endonuclease AfaI from Acidiphilium facilis, a new isoschizomer of RsaI

T2 - purification and properties

AU - Dou, Dexian

AU - Inagaki, Kenji

AU - Kita, Keiko

AU - Ohshima, Atsushi

AU - Hiraoka, Nobutugu

AU - Kishimoto, Noriaki

AU - Sugio, Tsuyoshi

AU - Tano, Tatsuo

PY - 1989/9/21

Y1 - 1989/9/21

N2 - We have purified AfaI endonuclease, an isoschizomer of RsaI, from Acidiphilium facilis strain 28H. The enzyme is homogeneous as judged by polyacrylamide gel electrophoresis, and composed of a single polypeptide chain with a molecular weight of 30 000. AfaI endonuclease, like RsaI, recognizes the tetranucleotide sequence 5′-G-T-A-C-3′, and cleaves between the T and A to produce blunt-ended fragments. The yield of the enzyme is 50-100-times that of the RsaI, which is form a phototrophic bacterium, Rhodospseudomonas sphaeroides strain 28/5.

AB - We have purified AfaI endonuclease, an isoschizomer of RsaI, from Acidiphilium facilis strain 28H. The enzyme is homogeneous as judged by polyacrylamide gel electrophoresis, and composed of a single polypeptide chain with a molecular weight of 30 000. AfaI endonuclease, like RsaI, recognizes the tetranucleotide sequence 5′-G-T-A-C-3′, and cleaves between the T and A to produce blunt-ended fragments. The yield of the enzyme is 50-100-times that of the RsaI, which is form a phototrophic bacterium, Rhodospseudomonas sphaeroides strain 28/5.

KW - (A. facilis)

KW - Acidophilic bacterium

KW - Enzyme purification

KW - Isoschizomer

KW - Restriction endonuclease

UR - http://www.scopus.com/inward/record.url?scp=0024975799&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024975799&partnerID=8YFLogxK

U2 - 10.1016/0167-4781(89)90082-1

DO - 10.1016/0167-4781(89)90082-1

M3 - Article

C2 - 2790034

AN - SCOPUS:0024975799

VL - 1009

SP - 83

EP - 86

JO - Biochimica et Biophysica Acta - Gene Structure and Expression

JF - Biochimica et Biophysica Acta - Gene Structure and Expression

SN - 0167-4781

IS - 1

ER -

Restriction endonuclease AfaI from Acidiphilium facilis, a new isoschizomer of RsaI: purification and properties

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Cite this