TY - JOUR
T1 - Restriction endonuclease AfaI from Acidiphilium facilis, a new isoschizomer of RsaI
T2 - purification and properties
AU - Dou, Dexian
AU - Inagaki, Kenji
AU - Kita, Keiko
AU - Ohshima, Atsushi
AU - Hiraoka, Nobutugu
AU - Kishimoto, Noriaki
AU - Sugio, Tsuyoshi
AU - Tano, Tatsuo
PY - 1989/9/21
Y1 - 1989/9/21
N2 - We have purified AfaI endonuclease, an isoschizomer of RsaI, from Acidiphilium facilis strain 28H. The enzyme is homogeneous as judged by polyacrylamide gel electrophoresis, and composed of a single polypeptide chain with a molecular weight of 30 000. AfaI endonuclease, like RsaI, recognizes the tetranucleotide sequence 5′-G-T-A-C-3′, and cleaves between the T and A to produce blunt-ended fragments. The yield of the enzyme is 50-100-times that of the RsaI, which is form a phototrophic bacterium, Rhodospseudomonas sphaeroides strain 28/5.
AB - We have purified AfaI endonuclease, an isoschizomer of RsaI, from Acidiphilium facilis strain 28H. The enzyme is homogeneous as judged by polyacrylamide gel electrophoresis, and composed of a single polypeptide chain with a molecular weight of 30 000. AfaI endonuclease, like RsaI, recognizes the tetranucleotide sequence 5′-G-T-A-C-3′, and cleaves between the T and A to produce blunt-ended fragments. The yield of the enzyme is 50-100-times that of the RsaI, which is form a phototrophic bacterium, Rhodospseudomonas sphaeroides strain 28/5.
KW - (A. facilis)
KW - Acidophilic bacterium
KW - Enzyme purification
KW - Isoschizomer
KW - Restriction endonuclease
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U2 - 10.1016/0167-4781(89)90082-1
DO - 10.1016/0167-4781(89)90082-1
M3 - Article
C2 - 2790034
AN - SCOPUS:0024975799
VL - 1009
SP - 83
EP - 86
JO - Biochimica et Biophysica Acta - Gene Structure and Expression
JF - Biochimica et Biophysica Acta - Gene Structure and Expression
SN - 0167-4781
IS - 1
ER -