RESPONSIVE-TO-ANTAGONIST1, a Menkes/Wilson disease-related copper transporter, is required for ethylene signaling in Arabidopsis

Takashi Hirayama, Joseph J. Kieber, Noriko Hirayama, Mikhail Kogan, Plinio Guzman, Saeid Nourizadeh, Jose M. Alonso, William P. Dailey, Andrew Dancis, Joseph R. Ecker

Research output: Contribution to journalArticle

282 Citations (Scopus)

Abstract

Ethylene is an important regulator of plant growth. We identified an Arabidopsis mutant, responsive-to-antagonist1 (ran1), that shows ethylene phenotypes in response to treatment with trans-cyclooctene, a potent receptor antagonist. Genetic epistasis studies revealed an early requirement for RAN1 in the ethylene pathway. RAN1 was cloned and found to encode a protein with similarity to copper-transporting P-type ATPases, including the human Menkes/Wilson proteins and yeast Ccc2p. Expression of RAN1 complemented the defects of a ccc2Δ mutant, demonstrating its function as a copper transporter. Transgenic CaMV 35S::RAN1 plants showed constitutive expression of ethylene responses, due to cosuppression of RAN1. These results provide an in planta demonstration that ethylene signaling requires copper and reveal that RAN1 acts by delivering copper to create functional hormone receptors.

Original languageEnglish
Pages (from-to)383-393
Number of pages11
JournalCell
Volume97
Issue number3
DOIs
Publication statusPublished - Apr 30 1999
Externally publishedYes

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Menkes Kinky Hair Syndrome
Hepatolenticular Degeneration
Arabidopsis
Copper
Genetic Epistasis
Plant Growth Regulators
Fungal Proteins
RNA Interference
Adenosine Triphosphatases
Demonstrations
Yeasts
ethylene
Hormones
Phenotype
Defects
Proteins

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

RESPONSIVE-TO-ANTAGONIST1, a Menkes/Wilson disease-related copper transporter, is required for ethylene signaling in Arabidopsis. / Hirayama, Takashi; Kieber, Joseph J.; Hirayama, Noriko; Kogan, Mikhail; Guzman, Plinio; Nourizadeh, Saeid; Alonso, Jose M.; Dailey, William P.; Dancis, Andrew; Ecker, Joseph R.

In: Cell, Vol. 97, No. 3, 30.04.1999, p. 383-393.

Research output: Contribution to journalArticle

Hirayama, T, Kieber, JJ, Hirayama, N, Kogan, M, Guzman, P, Nourizadeh, S, Alonso, JM, Dailey, WP, Dancis, A & Ecker, JR 1999, 'RESPONSIVE-TO-ANTAGONIST1, a Menkes/Wilson disease-related copper transporter, is required for ethylene signaling in Arabidopsis', Cell, vol. 97, no. 3, pp. 383-393. https://doi.org/10.1016/S0092-8674(00)80747-3
Hirayama, Takashi ; Kieber, Joseph J. ; Hirayama, Noriko ; Kogan, Mikhail ; Guzman, Plinio ; Nourizadeh, Saeid ; Alonso, Jose M. ; Dailey, William P. ; Dancis, Andrew ; Ecker, Joseph R. / RESPONSIVE-TO-ANTAGONIST1, a Menkes/Wilson disease-related copper transporter, is required for ethylene signaling in Arabidopsis. In: Cell. 1999 ; Vol. 97, No. 3. pp. 383-393.
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