Abstract
In a recent study, we found that TNF-α negatively regulates airway responsiveness through the activation of γδ T cells. The biological activities of TNF-α are mediated by two structurally related but functionally distinct receptors, p55 (TNFR1) and p75 (TNFR2), which are independently expressed on the cell surface. However, the relative importance of either TNFR in airway hyperresponsiveness (AHR) is unknown. To investigate the importance of these TNFRs in the development of allergen-induced AHR, p55-deficient and p75-deficient mice were sensitized to OVA by i.p. injection and subsequently challenged with OVA via the airways; airway responsiveness to inhaled methacholine was monitored. p75-deficient mice developed AHR to a similar degree as control mice. In contrast, p55-deficient mice, which were sensitized and challenged with OVA, failed to develop AHR. In p55-deficient mice, both the numbers of eosinophils and levels of IL-5 in bronchoalveolar lavage fluid were significantly lower than in sensitized/challenged control mice (p <0.05). However, depletion of γδ T cells resulted in significant increases in AHR in the p55-deficient mice, whereas no significant effect of γδ T cell depletion was evident in the p75-deficient mice. These data indicate that, in the absence of TNFR1 (p55), where TNF-α uses the p75 pathway exclusively, the development of AHR is regulated by γδ T cells.
| Original language | English |
|---|---|
| Pages (from-to) | 4190-4197 |
| Number of pages | 8 |
| Journal | Journal of Immunology |
| Volume | 169 |
| Issue number | 8 |
| Publication status | Published - Oct 15 2002 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Immunology
Cite this
Requirement for the p75 TNF-α receptor 2 in the regulation of airway hyperresponsiveness by γδ T cells. / Kanehiro, Arihiko; Lahn, Michael; Mäkelä, Mika J.; Dakhama, Azzeddine; Joetham, Anthony; Rha, Yeong Ho; Born, Willi; Gelfand, Erwin W.
In: Journal of Immunology, Vol. 169, No. 8, 15.10.2002, p. 4190-4197.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Requirement for the p75 TNF-α receptor 2 in the regulation of airway hyperresponsiveness by γδ T cells
AU - Kanehiro, Arihiko
AU - Lahn, Michael
AU - Mäkelä, Mika J.
AU - Dakhama, Azzeddine
AU - Joetham, Anthony
AU - Rha, Yeong Ho
AU - Born, Willi
AU - Gelfand, Erwin W.
PY - 2002/10/15
Y1 - 2002/10/15
N2 - In a recent study, we found that TNF-α negatively regulates airway responsiveness through the activation of γδ T cells. The biological activities of TNF-α are mediated by two structurally related but functionally distinct receptors, p55 (TNFR1) and p75 (TNFR2), which are independently expressed on the cell surface. However, the relative importance of either TNFR in airway hyperresponsiveness (AHR) is unknown. To investigate the importance of these TNFRs in the development of allergen-induced AHR, p55-deficient and p75-deficient mice were sensitized to OVA by i.p. injection and subsequently challenged with OVA via the airways; airway responsiveness to inhaled methacholine was monitored. p75-deficient mice developed AHR to a similar degree as control mice. In contrast, p55-deficient mice, which were sensitized and challenged with OVA, failed to develop AHR. In p55-deficient mice, both the numbers of eosinophils and levels of IL-5 in bronchoalveolar lavage fluid were significantly lower than in sensitized/challenged control mice (p <0.05). However, depletion of γδ T cells resulted in significant increases in AHR in the p55-deficient mice, whereas no significant effect of γδ T cell depletion was evident in the p75-deficient mice. These data indicate that, in the absence of TNFR1 (p55), where TNF-α uses the p75 pathway exclusively, the development of AHR is regulated by γδ T cells.
AB - In a recent study, we found that TNF-α negatively regulates airway responsiveness through the activation of γδ T cells. The biological activities of TNF-α are mediated by two structurally related but functionally distinct receptors, p55 (TNFR1) and p75 (TNFR2), which are independently expressed on the cell surface. However, the relative importance of either TNFR in airway hyperresponsiveness (AHR) is unknown. To investigate the importance of these TNFRs in the development of allergen-induced AHR, p55-deficient and p75-deficient mice were sensitized to OVA by i.p. injection and subsequently challenged with OVA via the airways; airway responsiveness to inhaled methacholine was monitored. p75-deficient mice developed AHR to a similar degree as control mice. In contrast, p55-deficient mice, which were sensitized and challenged with OVA, failed to develop AHR. In p55-deficient mice, both the numbers of eosinophils and levels of IL-5 in bronchoalveolar lavage fluid were significantly lower than in sensitized/challenged control mice (p <0.05). However, depletion of γδ T cells resulted in significant increases in AHR in the p55-deficient mice, whereas no significant effect of γδ T cell depletion was evident in the p75-deficient mice. These data indicate that, in the absence of TNFR1 (p55), where TNF-α uses the p75 pathway exclusively, the development of AHR is regulated by γδ T cells.
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UR - http://www.scopus.com/inward/citedby.url?scp=0037108360&partnerID=8YFLogxK
M3 - Article
C2 - 12370348
AN - SCOPUS:0037108360
VL - 169
SP - 4190
EP - 4197
JO - Journal of Immunology
JF - Journal of Immunology
SN - 0022-1767
IS - 8
ER -