Reproductive and developmental toxicity studies of toluene - II. Effects of inhalation exposure on fertility in rats

Atsushi Ono, Kiyoshi Sekita, Yukio Ogawa, Akihiko Hirose, Sachiko Suzuki, Minoru Saito, Katsushi Naito, Toyozo Kaneko, Tsuyoshi Furuya, Kunio Kawashima, Kazuo Yasuhara, Kiyoshi Matsumoto, Satoru Tanaka, Tohru Inoue, Yuji Kurokawa

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Male and female Sprague-Dawley rats were exposed to toluene vapor at 600 and 2000 ppm for 6 h/day, and effects on their fertility were investigated. Females were exposed from 14 days before mating until day 7 of gestation. Males were exposed for a total of 90 days, including the mating period; treatment was begun 60 days before pairing, and toxicity with respect to testicular and reproductive functions was examined. In females of the 2000 ppm-treated group, salivation and lacrimation that may have been caused by CNS depression were observed starting 20 days after exposure. Although no abnormalities were seen in mating behavior or fertility, fetal mortality and the number of dams with dead fetuses increased in the 2000 ppm group. In the males exposed to 2000 ppm toluene for 90 days, an increase in kidney weights and a decrease in thymus weights were observed. Basophilic changes and necrosis of kidney tubules were greater at the higher exposure level. Additionally, decreases in the weights of the epididymides and spermatic count were observed, indicating toxicity of toluene to the male reproductive system in vivo for the first time. In conclusion, embryo-fetal toxic effects were apparent in female rats exposed to toluene before and during the early stage of pregnancy. Subacute exposure to a high level (2000 ppm) of toluene vapor elicited mild toxic changes in the kidneys, thymus, and reproductive organs of males. Toxic effects on fertility and reproduction were thus demonstrated not only in females but also in males exposed to toluene vapor in the present study.

Original languageEnglish
Pages (from-to)9-20
Number of pages12
JournalJournal of Environmental Pathology, Toxicology and Oncology
Volume15
Issue number1
Publication statusPublished - 1996
Externally publishedYes

Fingerprint

Inhalation Exposure
Toluene
Fertility
Toxicity
Rats
Poisons
Thymus
Vapors
Weights and Measures
Thymus Gland
Kidney Tubules
Salivation
Kidney
Fetal Mortality
Pregnancy
Sperm Count
Epididymis
Dams
Reproduction
Sprague Dawley Rats

Keywords

  • Inhalant toxicity
  • Solvent abuse
  • Toluene toxicity

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Medicine(all)
  • Toxicology
  • Health, Toxicology and Mutagenesis

Cite this

Reproductive and developmental toxicity studies of toluene - II. Effects of inhalation exposure on fertility in rats. / Ono, Atsushi; Sekita, Kiyoshi; Ogawa, Yukio; Hirose, Akihiko; Suzuki, Sachiko; Saito, Minoru; Naito, Katsushi; Kaneko, Toyozo; Furuya, Tsuyoshi; Kawashima, Kunio; Yasuhara, Kazuo; Matsumoto, Kiyoshi; Tanaka, Satoru; Inoue, Tohru; Kurokawa, Yuji.

In: Journal of Environmental Pathology, Toxicology and Oncology, Vol. 15, No. 1, 1996, p. 9-20.

Research output: Contribution to journalArticle

Ono, A, Sekita, K, Ogawa, Y, Hirose, A, Suzuki, S, Saito, M, Naito, K, Kaneko, T, Furuya, T, Kawashima, K, Yasuhara, K, Matsumoto, K, Tanaka, S, Inoue, T & Kurokawa, Y 1996, 'Reproductive and developmental toxicity studies of toluene - II. Effects of inhalation exposure on fertility in rats', Journal of Environmental Pathology, Toxicology and Oncology, vol. 15, no. 1, pp. 9-20.
Ono, Atsushi ; Sekita, Kiyoshi ; Ogawa, Yukio ; Hirose, Akihiko ; Suzuki, Sachiko ; Saito, Minoru ; Naito, Katsushi ; Kaneko, Toyozo ; Furuya, Tsuyoshi ; Kawashima, Kunio ; Yasuhara, Kazuo ; Matsumoto, Kiyoshi ; Tanaka, Satoru ; Inoue, Tohru ; Kurokawa, Yuji. / Reproductive and developmental toxicity studies of toluene - II. Effects of inhalation exposure on fertility in rats. In: Journal of Environmental Pathology, Toxicology and Oncology. 1996 ; Vol. 15, No. 1. pp. 9-20.
@article{2d2ae57ca0bc4a91a6dab5c398ef3559,
title = "Reproductive and developmental toxicity studies of toluene - II. Effects of inhalation exposure on fertility in rats",
abstract = "Male and female Sprague-Dawley rats were exposed to toluene vapor at 600 and 2000 ppm for 6 h/day, and effects on their fertility were investigated. Females were exposed from 14 days before mating until day 7 of gestation. Males were exposed for a total of 90 days, including the mating period; treatment was begun 60 days before pairing, and toxicity with respect to testicular and reproductive functions was examined. In females of the 2000 ppm-treated group, salivation and lacrimation that may have been caused by CNS depression were observed starting 20 days after exposure. Although no abnormalities were seen in mating behavior or fertility, fetal mortality and the number of dams with dead fetuses increased in the 2000 ppm group. In the males exposed to 2000 ppm toluene for 90 days, an increase in kidney weights and a decrease in thymus weights were observed. Basophilic changes and necrosis of kidney tubules were greater at the higher exposure level. Additionally, decreases in the weights of the epididymides and spermatic count were observed, indicating toxicity of toluene to the male reproductive system in vivo for the first time. In conclusion, embryo-fetal toxic effects were apparent in female rats exposed to toluene before and during the early stage of pregnancy. Subacute exposure to a high level (2000 ppm) of toluene vapor elicited mild toxic changes in the kidneys, thymus, and reproductive organs of males. Toxic effects on fertility and reproduction were thus demonstrated not only in females but also in males exposed to toluene vapor in the present study.",
keywords = "Inhalant toxicity, Solvent abuse, Toluene toxicity",
author = "Atsushi Ono and Kiyoshi Sekita and Yukio Ogawa and Akihiko Hirose and Sachiko Suzuki and Minoru Saito and Katsushi Naito and Toyozo Kaneko and Tsuyoshi Furuya and Kunio Kawashima and Kazuo Yasuhara and Kiyoshi Matsumoto and Satoru Tanaka and Tohru Inoue and Yuji Kurokawa",
year = "1996",
language = "English",
volume = "15",
pages = "9--20",
journal = "Journal of Environmental Pathology, Toxicology and Oncology",
issn = "0731-8898",
publisher = "Begell House Inc.",
number = "1",

}

TY - JOUR

T1 - Reproductive and developmental toxicity studies of toluene - II. Effects of inhalation exposure on fertility in rats

AU - Ono, Atsushi

AU - Sekita, Kiyoshi

AU - Ogawa, Yukio

AU - Hirose, Akihiko

AU - Suzuki, Sachiko

AU - Saito, Minoru

AU - Naito, Katsushi

AU - Kaneko, Toyozo

AU - Furuya, Tsuyoshi

AU - Kawashima, Kunio

AU - Yasuhara, Kazuo

AU - Matsumoto, Kiyoshi

AU - Tanaka, Satoru

AU - Inoue, Tohru

AU - Kurokawa, Yuji

PY - 1996

Y1 - 1996

N2 - Male and female Sprague-Dawley rats were exposed to toluene vapor at 600 and 2000 ppm for 6 h/day, and effects on their fertility were investigated. Females were exposed from 14 days before mating until day 7 of gestation. Males were exposed for a total of 90 days, including the mating period; treatment was begun 60 days before pairing, and toxicity with respect to testicular and reproductive functions was examined. In females of the 2000 ppm-treated group, salivation and lacrimation that may have been caused by CNS depression were observed starting 20 days after exposure. Although no abnormalities were seen in mating behavior or fertility, fetal mortality and the number of dams with dead fetuses increased in the 2000 ppm group. In the males exposed to 2000 ppm toluene for 90 days, an increase in kidney weights and a decrease in thymus weights were observed. Basophilic changes and necrosis of kidney tubules were greater at the higher exposure level. Additionally, decreases in the weights of the epididymides and spermatic count were observed, indicating toxicity of toluene to the male reproductive system in vivo for the first time. In conclusion, embryo-fetal toxic effects were apparent in female rats exposed to toluene before and during the early stage of pregnancy. Subacute exposure to a high level (2000 ppm) of toluene vapor elicited mild toxic changes in the kidneys, thymus, and reproductive organs of males. Toxic effects on fertility and reproduction were thus demonstrated not only in females but also in males exposed to toluene vapor in the present study.

AB - Male and female Sprague-Dawley rats were exposed to toluene vapor at 600 and 2000 ppm for 6 h/day, and effects on their fertility were investigated. Females were exposed from 14 days before mating until day 7 of gestation. Males were exposed for a total of 90 days, including the mating period; treatment was begun 60 days before pairing, and toxicity with respect to testicular and reproductive functions was examined. In females of the 2000 ppm-treated group, salivation and lacrimation that may have been caused by CNS depression were observed starting 20 days after exposure. Although no abnormalities were seen in mating behavior or fertility, fetal mortality and the number of dams with dead fetuses increased in the 2000 ppm group. In the males exposed to 2000 ppm toluene for 90 days, an increase in kidney weights and a decrease in thymus weights were observed. Basophilic changes and necrosis of kidney tubules were greater at the higher exposure level. Additionally, decreases in the weights of the epididymides and spermatic count were observed, indicating toxicity of toluene to the male reproductive system in vivo for the first time. In conclusion, embryo-fetal toxic effects were apparent in female rats exposed to toluene before and during the early stage of pregnancy. Subacute exposure to a high level (2000 ppm) of toluene vapor elicited mild toxic changes in the kidneys, thymus, and reproductive organs of males. Toxic effects on fertility and reproduction were thus demonstrated not only in females but also in males exposed to toluene vapor in the present study.

KW - Inhalant toxicity

KW - Solvent abuse

KW - Toluene toxicity

UR - http://www.scopus.com/inward/record.url?scp=0030485164&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030485164&partnerID=8YFLogxK

M3 - Article

C2 - 9037260

AN - SCOPUS:0030485164

VL - 15

SP - 9

EP - 20

JO - Journal of Environmental Pathology, Toxicology and Oncology

JF - Journal of Environmental Pathology, Toxicology and Oncology

SN - 0731-8898

IS - 1

ER -