Repression of in vitro transcription of the Escherichia coli fnr and nar X genes by FNR protein

Kojiro Takahashi, Takako Hattori, Tohru Nakanishi, Tsutomu Nohno, Nobuyuki Fujita, Akira Ishihama, Shigehiko Taniguchi

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

In facultative anaerobes, the anaerobic expression of respiratory genes is regulated by a transcriptional activator, FNR. Transcription in vitro of the E. coli fnr gene was repressed by its product, FNR. The transcription of the E. coli nar X gene encoding the nitrate sensor protein was likewise repressed. DNA truncation experiments for fnr and nar X genes indicated that multiple anaero-boxes in each promoter region are essential for repression by the FNR protein, but they also suggest that factor-independent upstream activation signals are operating with these promoters.

Original languageEnglish
Pages (from-to)59-64
Number of pages6
JournalFEBS Letters
Volume340
Issue number1-2
DOIs
Publication statusPublished - Feb 28 1994

Keywords

  • Escherichia coli
  • FNR
  • Transcriptional regulation
  • Upstream activation signal
  • fnr, nar X

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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  • Cite this

    Takahashi, K., Hattori, T., Nakanishi, T., Nohno, T., Fujita, N., Ishihama, A., & Taniguchi, S. (1994). Repression of in vitro transcription of the Escherichia coli fnr and nar X genes by FNR protein. FEBS Letters, 340(1-2), 59-64. https://doi.org/10.1016/0014-5793(94)80173-8