Repression of in vitro transcription of the Escherichia coli fnr and nar X genes by FNR protein

Kojiro Takahashi, Takako Hattori, Tohru Nakanishi, Tsutomu Nohno, Nobuyuki Fujita, Akira Ishihama, Shigehiko Taniguchi

Research output: Contribution to journal › Article

17 Citations (Scopus)

Abstract

In facultative anaerobes, the anaerobic expression of respiratory genes is regulated by a transcriptional activator, FNR. Transcription in vitro of the E. coli fnr gene was repressed by its product, FNR. The transcription of the E. coli nar X gene encoding the nitrate sensor protein was likewise repressed. DNA truncation experiments for fnr and nar X genes indicated that multiple anaero-boxes in each promoter region are essential for repression by the FNR protein, but they also suggest that factor-independent upstream activation signals are operating with these promoters.

Original languageEnglish
Pages (from-to)59-64
Number of pages6
JournalFEBS Letters
Volume340
Issue number1-2
DOIs
Publication statusPublished - Feb 28 1994

Fingerprint

Transcription
Escherichia coli
Genes
Proteins
Gene encoding
Genetic Promoter Regions
Nitrates
Byproducts
Chemical activation
Gene Expression
DNA
Sensors
In Vitro Techniques
Experiments

Keywords

  • Escherichia coli
  • FNR
  • fnr, nar X
  • Transcriptional regulation
  • Upstream activation signal

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Takahashi, K., Hattori, T., Nakanishi, T., Nohno, T., Fujita, N., Ishihama, A., & Taniguchi, S. (1994). Repression of in vitro transcription of the Escherichia coli fnr and nar X genes by FNR protein. FEBS Letters, 340(1-2), 59-64. https://doi.org/10.1016/0014-5793(94)80173-8

Repression of in vitro transcription of the Escherichia coli fnr and nar X genes by FNR protein. / Takahashi, Kojiro; Hattori, Takako; Nakanishi, Tohru; Nohno, Tsutomu; Fujita, Nobuyuki; Ishihama, Akira; Taniguchi, Shigehiko.

In: FEBS Letters, Vol. 340, No. 1-2, 28.02.1994, p. 59-64.

Research output: Contribution to journal › Article

Takahashi, K, Hattori, T, Nakanishi, T, Nohno, T, Fujita, N, Ishihama, A & Taniguchi, S 1994, 'Repression of in vitro transcription of the Escherichia coli fnr and nar X genes by FNR protein', FEBS Letters, vol. 340, no. 1-2, pp. 59-64. https://doi.org/10.1016/0014-5793(94)80173-8
Takahashi K, Hattori T, Nakanishi T, Nohno T, Fujita N, Ishihama A et al. Repression of in vitro transcription of the Escherichia coli fnr and nar X genes by FNR protein. FEBS Letters. 1994 Feb 28;340(1-2):59-64. https://doi.org/10.1016/0014-5793(94)80173-8
Takahashi, Kojiro ; Hattori, Takako ; Nakanishi, Tohru ; Nohno, Tsutomu ; Fujita, Nobuyuki ; Ishihama, Akira ; Taniguchi, Shigehiko. / Repression of in vitro transcription of the Escherichia coli fnr and nar X genes by FNR protein. In: FEBS Letters. 1994 ; Vol. 340, No. 1-2. pp. 59-64.
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