TY - JOUR
T1 - Repression of in vitro transcription of the Escherichia coli fnr and nar X genes by FNR protein
AU - Takahashi, Kojiro
AU - Hattori, Takako
AU - Nakanishi, Tohru
AU - Nohno, Tsutomu
AU - Fujita, Nobuyuki
AU - Ishihama, Akira
AU - Taniguchi, Shigehiko
N1 - Funding Information:
Acknowfedgeme~~s: We thank Drs. Y. Kohara (National Instituteo f Genetics,J apan), S. Tabor (Harvard Medicat School, USA) and H. Weiner( PurdueU niversity,U SA) for kindly donatingl ambda8 C12, pT7-6a nd BLZl(DE3)pLysS strains,r espectivelyT.h is work was sup-portedb y Grants-in-Aidf or ScientificR esearcho n Priority Areas( no. 022632090, 32592100, 4254212to K.T.; no 033540350, 4256105, 0445461t4o AI.) from the JapaneseM inistry of Education,S cience and Culture,a nd by a fund (to S.T.) from CIBA-GEIGY Foundation for the Promotiono f Science (1991).
PY - 1994/2/28
Y1 - 1994/2/28
N2 - In facultative anaerobes, the anaerobic expression of respiratory genes is regulated by a transcriptional activator, FNR. Transcription in vitro of the E. coli fnr gene was repressed by its product, FNR. The transcription of the E. coli nar X gene encoding the nitrate sensor protein was likewise repressed. DNA truncation experiments for fnr and nar X genes indicated that multiple anaero-boxes in each promoter region are essential for repression by the FNR protein, but they also suggest that factor-independent upstream activation signals are operating with these promoters.
AB - In facultative anaerobes, the anaerobic expression of respiratory genes is regulated by a transcriptional activator, FNR. Transcription in vitro of the E. coli fnr gene was repressed by its product, FNR. The transcription of the E. coli nar X gene encoding the nitrate sensor protein was likewise repressed. DNA truncation experiments for fnr and nar X genes indicated that multiple anaero-boxes in each promoter region are essential for repression by the FNR protein, but they also suggest that factor-independent upstream activation signals are operating with these promoters.
KW - Escherichia coli
KW - FNR
KW - Transcriptional regulation
KW - Upstream activation signal
KW - fnr, nar X
UR - http://www.scopus.com/inward/record.url?scp=0028265802&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028265802&partnerID=8YFLogxK
U2 - 10.1016/0014-5793(94)80173-8
DO - 10.1016/0014-5793(94)80173-8
M3 - Article
C2 - 8119409
AN - SCOPUS:0028265802
VL - 340
SP - 59
EP - 64
JO - FEBS Letters
JF - FEBS Letters
SN - 0014-5793
IS - 1-2
ER -