Replication study of 15 recently published loci for body fat distribution in the Japanese population

Kikuko Hotta, Aya Kitamoto, Takuya Kitamoto, Seiho Mizusawa, Hajime Teranishi, Rina So, Tomoaki Matsuo, Yoshio Nakata, Hideyuki Hyogo, Hidenori Ochi, Takahiro Nakamura, Seika Kamohara, Nobuyuki Miyatake, Kazuaki Kotani, Naoto Itoh, Ikuo Mineo, Jun Wada, Masato Yoneda, Atsushi Nakajima, Tohru FunahashiShigeru Miyazaki, Katsuto Tokunaga, Hiroaki Masuzaki, Takato Ueno, Kazuaki Chayama, Kazuyuki Hamaguchi, Kentaro Yamada, Toshiaki Hanafusa, Shinichi Oikawa, Toshiie Sakata, Kiyoji Tanaka, Yuji Matsuzawa, Kazuwa Nakao, Akihiro Sekine

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Aim: Visceral fat accumulation plays an integral role in morbidity and mortality rates by increasing the risk of developing metabolic disorders such as type 2 diabetes, dyslipidemia, and hypertension. New genetic loci associated with fat distribution, measured by waist-hip ratios and computed tomography (CT), have recently been identified by genome-wide association studies in European-descent populations. This study used CT to investigate whether single nucleotide polymorphisms (SNPs) that confer susceptibility to fat distribution are associated with visceral fat area (VFA) and subcutaneous fat area (SFA) in the Japanese population. Methods: We measured the VFAs and SFAs of 1424 obese Japanese subjects (BMI ≥25 kg/m2, 635 men and 789 women) that were genotyped at 15 SNPs, namely, TBX15 rs984222, DNM3 rs1011731, LYPLAL1 rs4846567, GRB14 rs10195252, NISCH rs6784615, ADAMTS9 rs6795735, CPEB4 rs6861681, LY86 rs1294421, VEGFA rs6905288, RSPO3 rs9491696, NFE2L3 rs1055144, ITPR2 rs718314, HOXC13 rs1443512, ZNRF3 rs4823006 and THNSL2 rs1659258. Results: The G-allele of LYPLAL1 rs4846567 was borderline associated with an increased ratio of VFA to SFA (V/S ratio; p=0.0020). LYPLAL1 rs4846567 had a stronger effect on the V/S ratio in women (p=0.0078) than in men (p= 0.12); however, neither result was significant after Bonferroni correction for multiple comparisons. NISCH rs6784615 was nominally associated with increased VFA (p=0.040) and V/S ratio (p= 0.020). The other SNPs analyzed were not significantly associated with body mass index (BMI), VFA, or SFA. Conclusion: Our results suggest that LYPLAL1 rs4846567 and NISCH rs6784615 may influence fat distribution in the Japanese population.

Original languageEnglish
Pages (from-to)336-350
Number of pages15
JournalJournal of Atherosclerosis and Thrombosis
Volume20
Issue number4
DOIs
Publication statusPublished - 2013

Fingerprint

Body Fat Distribution
Intra-Abdominal Fat
Fats
Subcutaneous Fat
Single Nucleotide Polymorphism
Population
Body Mass Index
Tomography
Polymorphism
Genetic Loci
Waist-Hip Ratio
Genome-Wide Association Study
Nucleotides
Dyslipidemias
Type 2 Diabetes Mellitus
Alleles
Hypertension
Morbidity
Mortality
Medical problems

Keywords

  • Computed tomography
  • Japanese subjects
  • LYPLAL1
  • Subcutaneous fat area
  • Visceral fat area

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Internal Medicine
  • Biochemistry, medical

Cite this

Hotta, K., Kitamoto, A., Kitamoto, T., Mizusawa, S., Teranishi, H., So, R., ... Sekine, A. (2013). Replication study of 15 recently published loci for body fat distribution in the Japanese population. Journal of Atherosclerosis and Thrombosis, 20(4), 336-350. https://doi.org/10.5551/jat.14589

Replication study of 15 recently published loci for body fat distribution in the Japanese population. / Hotta, Kikuko; Kitamoto, Aya; Kitamoto, Takuya; Mizusawa, Seiho; Teranishi, Hajime; So, Rina; Matsuo, Tomoaki; Nakata, Yoshio; Hyogo, Hideyuki; Ochi, Hidenori; Nakamura, Takahiro; Kamohara, Seika; Miyatake, Nobuyuki; Kotani, Kazuaki; Itoh, Naoto; Mineo, Ikuo; Wada, Jun; Yoneda, Masato; Nakajima, Atsushi; Funahashi, Tohru; Miyazaki, Shigeru; Tokunaga, Katsuto; Masuzaki, Hiroaki; Ueno, Takato; Chayama, Kazuaki; Hamaguchi, Kazuyuki; Yamada, Kentaro; Hanafusa, Toshiaki; Oikawa, Shinichi; Sakata, Toshiie; Tanaka, Kiyoji; Matsuzawa, Yuji; Nakao, Kazuwa; Sekine, Akihiro.

In: Journal of Atherosclerosis and Thrombosis, Vol. 20, No. 4, 2013, p. 336-350.

Research output: Contribution to journalArticle

Hotta, K, Kitamoto, A, Kitamoto, T, Mizusawa, S, Teranishi, H, So, R, Matsuo, T, Nakata, Y, Hyogo, H, Ochi, H, Nakamura, T, Kamohara, S, Miyatake, N, Kotani, K, Itoh, N, Mineo, I, Wada, J, Yoneda, M, Nakajima, A, Funahashi, T, Miyazaki, S, Tokunaga, K, Masuzaki, H, Ueno, T, Chayama, K, Hamaguchi, K, Yamada, K, Hanafusa, T, Oikawa, S, Sakata, T, Tanaka, K, Matsuzawa, Y, Nakao, K & Sekine, A 2013, 'Replication study of 15 recently published loci for body fat distribution in the Japanese population', Journal of Atherosclerosis and Thrombosis, vol. 20, no. 4, pp. 336-350. https://doi.org/10.5551/jat.14589
Hotta, Kikuko ; Kitamoto, Aya ; Kitamoto, Takuya ; Mizusawa, Seiho ; Teranishi, Hajime ; So, Rina ; Matsuo, Tomoaki ; Nakata, Yoshio ; Hyogo, Hideyuki ; Ochi, Hidenori ; Nakamura, Takahiro ; Kamohara, Seika ; Miyatake, Nobuyuki ; Kotani, Kazuaki ; Itoh, Naoto ; Mineo, Ikuo ; Wada, Jun ; Yoneda, Masato ; Nakajima, Atsushi ; Funahashi, Tohru ; Miyazaki, Shigeru ; Tokunaga, Katsuto ; Masuzaki, Hiroaki ; Ueno, Takato ; Chayama, Kazuaki ; Hamaguchi, Kazuyuki ; Yamada, Kentaro ; Hanafusa, Toshiaki ; Oikawa, Shinichi ; Sakata, Toshiie ; Tanaka, Kiyoji ; Matsuzawa, Yuji ; Nakao, Kazuwa ; Sekine, Akihiro. / Replication study of 15 recently published loci for body fat distribution in the Japanese population. In: Journal of Atherosclerosis and Thrombosis. 2013 ; Vol. 20, No. 4. pp. 336-350.
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title = "Replication study of 15 recently published loci for body fat distribution in the Japanese population",
abstract = "Aim: Visceral fat accumulation plays an integral role in morbidity and mortality rates by increasing the risk of developing metabolic disorders such as type 2 diabetes, dyslipidemia, and hypertension. New genetic loci associated with fat distribution, measured by waist-hip ratios and computed tomography (CT), have recently been identified by genome-wide association studies in European-descent populations. This study used CT to investigate whether single nucleotide polymorphisms (SNPs) that confer susceptibility to fat distribution are associated with visceral fat area (VFA) and subcutaneous fat area (SFA) in the Japanese population. Methods: We measured the VFAs and SFAs of 1424 obese Japanese subjects (BMI ≥25 kg/m2, 635 men and 789 women) that were genotyped at 15 SNPs, namely, TBX15 rs984222, DNM3 rs1011731, LYPLAL1 rs4846567, GRB14 rs10195252, NISCH rs6784615, ADAMTS9 rs6795735, CPEB4 rs6861681, LY86 rs1294421, VEGFA rs6905288, RSPO3 rs9491696, NFE2L3 rs1055144, ITPR2 rs718314, HOXC13 rs1443512, ZNRF3 rs4823006 and THNSL2 rs1659258. Results: The G-allele of LYPLAL1 rs4846567 was borderline associated with an increased ratio of VFA to SFA (V/S ratio; p=0.0020). LYPLAL1 rs4846567 had a stronger effect on the V/S ratio in women (p=0.0078) than in men (p= 0.12); however, neither result was significant after Bonferroni correction for multiple comparisons. NISCH rs6784615 was nominally associated with increased VFA (p=0.040) and V/S ratio (p= 0.020). The other SNPs analyzed were not significantly associated with body mass index (BMI), VFA, or SFA. Conclusion: Our results suggest that LYPLAL1 rs4846567 and NISCH rs6784615 may influence fat distribution in the Japanese population.",
keywords = "Computed tomography, Japanese subjects, LYPLAL1, Subcutaneous fat area, Visceral fat area",
author = "Kikuko Hotta and Aya Kitamoto and Takuya Kitamoto and Seiho Mizusawa and Hajime Teranishi and Rina So and Tomoaki Matsuo and Yoshio Nakata and Hideyuki Hyogo and Hidenori Ochi and Takahiro Nakamura and Seika Kamohara and Nobuyuki Miyatake and Kazuaki Kotani and Naoto Itoh and Ikuo Mineo and Jun Wada and Masato Yoneda and Atsushi Nakajima and Tohru Funahashi and Shigeru Miyazaki and Katsuto Tokunaga and Hiroaki Masuzaki and Takato Ueno and Kazuaki Chayama and Kazuyuki Hamaguchi and Kentaro Yamada and Toshiaki Hanafusa and Shinichi Oikawa and Toshiie Sakata and Kiyoji Tanaka and Yuji Matsuzawa and Kazuwa Nakao and Akihiro Sekine",
year = "2013",
doi = "10.5551/jat.14589",
language = "English",
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pages = "336--350",
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TY - JOUR

T1 - Replication study of 15 recently published loci for body fat distribution in the Japanese population

AU - Hotta, Kikuko

AU - Kitamoto, Aya

AU - Kitamoto, Takuya

AU - Mizusawa, Seiho

AU - Teranishi, Hajime

AU - So, Rina

AU - Matsuo, Tomoaki

AU - Nakata, Yoshio

AU - Hyogo, Hideyuki

AU - Ochi, Hidenori

AU - Nakamura, Takahiro

AU - Kamohara, Seika

AU - Miyatake, Nobuyuki

AU - Kotani, Kazuaki

AU - Itoh, Naoto

AU - Mineo, Ikuo

AU - Wada, Jun

AU - Yoneda, Masato

AU - Nakajima, Atsushi

AU - Funahashi, Tohru

AU - Miyazaki, Shigeru

AU - Tokunaga, Katsuto

AU - Masuzaki, Hiroaki

AU - Ueno, Takato

AU - Chayama, Kazuaki

AU - Hamaguchi, Kazuyuki

AU - Yamada, Kentaro

AU - Hanafusa, Toshiaki

AU - Oikawa, Shinichi

AU - Sakata, Toshiie

AU - Tanaka, Kiyoji

AU - Matsuzawa, Yuji

AU - Nakao, Kazuwa

AU - Sekine, Akihiro

PY - 2013

Y1 - 2013

N2 - Aim: Visceral fat accumulation plays an integral role in morbidity and mortality rates by increasing the risk of developing metabolic disorders such as type 2 diabetes, dyslipidemia, and hypertension. New genetic loci associated with fat distribution, measured by waist-hip ratios and computed tomography (CT), have recently been identified by genome-wide association studies in European-descent populations. This study used CT to investigate whether single nucleotide polymorphisms (SNPs) that confer susceptibility to fat distribution are associated with visceral fat area (VFA) and subcutaneous fat area (SFA) in the Japanese population. Methods: We measured the VFAs and SFAs of 1424 obese Japanese subjects (BMI ≥25 kg/m2, 635 men and 789 women) that were genotyped at 15 SNPs, namely, TBX15 rs984222, DNM3 rs1011731, LYPLAL1 rs4846567, GRB14 rs10195252, NISCH rs6784615, ADAMTS9 rs6795735, CPEB4 rs6861681, LY86 rs1294421, VEGFA rs6905288, RSPO3 rs9491696, NFE2L3 rs1055144, ITPR2 rs718314, HOXC13 rs1443512, ZNRF3 rs4823006 and THNSL2 rs1659258. Results: The G-allele of LYPLAL1 rs4846567 was borderline associated with an increased ratio of VFA to SFA (V/S ratio; p=0.0020). LYPLAL1 rs4846567 had a stronger effect on the V/S ratio in women (p=0.0078) than in men (p= 0.12); however, neither result was significant after Bonferroni correction for multiple comparisons. NISCH rs6784615 was nominally associated with increased VFA (p=0.040) and V/S ratio (p= 0.020). The other SNPs analyzed were not significantly associated with body mass index (BMI), VFA, or SFA. Conclusion: Our results suggest that LYPLAL1 rs4846567 and NISCH rs6784615 may influence fat distribution in the Japanese population.

AB - Aim: Visceral fat accumulation plays an integral role in morbidity and mortality rates by increasing the risk of developing metabolic disorders such as type 2 diabetes, dyslipidemia, and hypertension. New genetic loci associated with fat distribution, measured by waist-hip ratios and computed tomography (CT), have recently been identified by genome-wide association studies in European-descent populations. This study used CT to investigate whether single nucleotide polymorphisms (SNPs) that confer susceptibility to fat distribution are associated with visceral fat area (VFA) and subcutaneous fat area (SFA) in the Japanese population. Methods: We measured the VFAs and SFAs of 1424 obese Japanese subjects (BMI ≥25 kg/m2, 635 men and 789 women) that were genotyped at 15 SNPs, namely, TBX15 rs984222, DNM3 rs1011731, LYPLAL1 rs4846567, GRB14 rs10195252, NISCH rs6784615, ADAMTS9 rs6795735, CPEB4 rs6861681, LY86 rs1294421, VEGFA rs6905288, RSPO3 rs9491696, NFE2L3 rs1055144, ITPR2 rs718314, HOXC13 rs1443512, ZNRF3 rs4823006 and THNSL2 rs1659258. Results: The G-allele of LYPLAL1 rs4846567 was borderline associated with an increased ratio of VFA to SFA (V/S ratio; p=0.0020). LYPLAL1 rs4846567 had a stronger effect on the V/S ratio in women (p=0.0078) than in men (p= 0.12); however, neither result was significant after Bonferroni correction for multiple comparisons. NISCH rs6784615 was nominally associated with increased VFA (p=0.040) and V/S ratio (p= 0.020). The other SNPs analyzed were not significantly associated with body mass index (BMI), VFA, or SFA. Conclusion: Our results suggest that LYPLAL1 rs4846567 and NISCH rs6784615 may influence fat distribution in the Japanese population.

KW - Computed tomography

KW - Japanese subjects

KW - LYPLAL1

KW - Subcutaneous fat area

KW - Visceral fat area

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