Replication of hepatitis C virus genome

Research output: Contribution to journalArticle

Abstract

The studies on the mechanism of HCV replication proliferated after the development of cell culture based-subgenomic HCV replicon system and genome-length HCV RNA replication system. Furthermore, these RNA replication systems have been improved to be suitable systems for the screening of anti-HCV reagents by the introduction of reporter genes such as luciferase. Genetic analysis of HCV RNAs obtained in long-term cell culture of HCV replicon or genome-length HCV RNA-harboring cells revealed that genetic mutations in HCV RNAs accumulated in a time-dependent manner. The genetic diversity of HCVs was also enlarged in a time-dependent manner. The appearance of adaptive mutation in HCV replicon or genome-length HCV RNA is one of characteristic features of HCV RNA replication system. Although human hepatoma-derived HuH-7 cell line was mainly used for HCV RNA replication systems, a specific combination of adaptive mutations led to develop the HCV RNA replication systems using a new human hepatoma cell line other than HuH-7.

Original languageEnglish
Pages (from-to)191-198
Number of pages8
JournalUirusu. Journal of virology
Volume58
Issue number2
DOIs
Publication statusPublished - Dec 2008

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Hepacivirus
Genome
RNA
Replicon
Mutation
Hepatocellular Carcinoma
Cell Culture Techniques
Cell Line
Luciferases
Reporter Genes

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Replication of hepatitis C virus genome. / Kato, Nobuyuki.

In: Uirusu. Journal of virology, Vol. 58, No. 2, 12.2008, p. 191-198.

Research output: Contribution to journalArticle

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