Replication of hepatitis B virus which carries foreign DNA in vitro

Tadashi Hanafusa, Yasuhiro Yumoto, Hajime Hada, Toshiyuki Shinji, Norio Koide, Takao Tsuji

    Research output: Contribution to journalArticlepeer-review

    5 Citations (Scopus)

    Abstract

    Targeting a specific DNA sequence to the desired tissues is an important step in gene therapy. The hepatitis B virus (HBV) is the only DNA virus that has hepatocyte specificity. We attempted to construct an HBV-based vector for targeting the liver. We observed the replication and secretion of virus particles in an HBV construct that lacks X gene and carries an extra 63 bp DNA fragment in vitro. Replication was observed in the cell line HuH-7 but not HepG2. From this construct, we designed an HBV-based vector that could carry foreign DNA. HBV based vectors provide for the possibilities of generating therapeutic agents for individual patients. Our host vector system may be used to clear out the HBV from the HBV carrier or chronic hepatitis B patients by introducing a genetically engineered HBV into these patients.

    Original languageEnglish
    Pages (from-to)530-533
    Number of pages4
    JournalBiochemical and Biophysical Research Communications
    Volume262
    Issue number2
    DOIs
    Publication statusPublished - Aug 27 1999

    ASJC Scopus subject areas

    • Biophysics
    • Biochemistry
    • Molecular Biology
    • Cell Biology

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