Replicating Marek's disease virus (MDV) serotype 2 DNA with inserted MDV serotype 1 DNA sequences in a Marek's disease lymphoblastoid cell line MSB1-41C

K. Hirai, Masao Yamada, Yujiro Arao, S. Kato, S. Nii

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

We characterized the properties of herpes-type viruses which grew well in a Marek's disease lymphoblastoid cell line, MSB1-41C, inducing cytopathic effect characterized by the formation of syncytial giant cells. Examination of the infectious virus by field inversion gel electrophoresis revealed the presence of DNA of about 180 kbp in both the culture fluid and cell fractions of the infected MSB-41C cells. The DNA was found to consist of Marek's disease virus (MDV) serotype 2 (MDV2) and MDV serotype 1 (MDV1) DNA by Southern blot hybridization. The MDV1 DNA consisted of sequences mainly from the long inverted repeats including multiple copies of 132 bp direct tandem repeats. Molecular cloning of BamHI digests of the MDV2 DNA revealed a fragment of MDV1 DNA and MDV2 DNA fused together, indicating that the recombinant MDV2 DNA had been generated by genetic recombination with the latent MDV1 DNA.

Original languageEnglish
Pages (from-to)153-165
Number of pages13
JournalArchives of Virology
Volume114
Issue number3-4
DOIs
Publication statusPublished - Sep 1990

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Gallid Herpesvirus 3
Gallid Herpesvirus 2
Marek Disease
Cell Line
DNA
Viruses
Tandem Repeat Sequences
Pulsed Field Gel Electrophoresis
Molecular Cloning
Giant Cells
Southern Blotting
Genetic Recombination
Serogroup
Cell Culture Techniques

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Genetics

Cite this

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title = "Replicating Marek's disease virus (MDV) serotype 2 DNA with inserted MDV serotype 1 DNA sequences in a Marek's disease lymphoblastoid cell line MSB1-41C",
abstract = "We characterized the properties of herpes-type viruses which grew well in a Marek's disease lymphoblastoid cell line, MSB1-41C, inducing cytopathic effect characterized by the formation of syncytial giant cells. Examination of the infectious virus by field inversion gel electrophoresis revealed the presence of DNA of about 180 kbp in both the culture fluid and cell fractions of the infected MSB-41C cells. The DNA was found to consist of Marek's disease virus (MDV) serotype 2 (MDV2) and MDV serotype 1 (MDV1) DNA by Southern blot hybridization. The MDV1 DNA consisted of sequences mainly from the long inverted repeats including multiple copies of 132 bp direct tandem repeats. Molecular cloning of BamHI digests of the MDV2 DNA revealed a fragment of MDV1 DNA and MDV2 DNA fused together, indicating that the recombinant MDV2 DNA had been generated by genetic recombination with the latent MDV1 DNA.",
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AU - Arao, Yujiro

AU - Kato, S.

AU - Nii, S.

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