Repetitive light pulse-induced photoinhibition of photosystem i severely affects CO2 assimilation and photoprotection in wheat leaves

Marek Zivcak, Marian Brestic, Kristyna Kunderlikova, Oksana Sytar, Suleyman Allakhverdiev

Research output: Contribution to journalArticle

74 Citations (Scopus)

Abstract

It was previously found that photosystem I (PSI) photoinhibition represents mostly irreversible damage with a slow recovery; however, its physiological significance has not been sufficiently characterized. The aim of the study was to assess the effect of PSI photoinhibition on photosynthesis in vivo. The inactivation of PSI was done by a series of short light saturation pulses applied by fluorimeter in darkness (every 10 s for 15 min), which led to decrease of both PSI (~60 %) and photosystem II (PSII) (~15 %) photochemical activity. No PSI recovery was observed within 2 days, whereas the PSII was fully recovered. Strongly limited PSI electron transport led to an imbalance between PSII and PSI photochemistry, with a high excitation pressure on PSII acceptor side and low oxidation of the PSI donor side. Low and delayed light-induced NPQ and P700+ rise in inactivated samples indicated a decrease in formation of transthylakoid proton gradient (ΔpH), which was confirmed also by analysis of electrochromic bandshift (ECSt) records. In parallel with photochemical parameters, the CO2 assimilation was also strongly inhibited, more in low light (~70 %) than in high light (~45 %); the decrease was not caused by stomatal closure. PSI electron transport limited the CO2 assimilation at low to moderate light intensities, but it seems not to be directly responsible for a low CO2 assimilation at high light. In this regard, the possible effects of PSI photoinhibition on the redox signaling in chloroplast and its role in downregulation of Calvin cycle activity are discussed.

Original languageEnglish
Pages (from-to)449-463
Number of pages15
JournalPhotosynthesis Research
Volume126
Issue number2-3
DOIs
Publication statusPublished - Dec 1 2015
Externally publishedYes

Fingerprint

Photosystem I Protein Complex
photostability
photosystem I
photoinhibition
Triticum
assimilation (physiology)
Light
wheat
Photosystem II Protein Complex
leaves
photosystem II
Photosynthesis
Electron Transport
electron transfer
Fluorometers
Photochemistry
Recovery
Proton-Motive Force
Calvin cycle
photochemistry

Keywords

  • Electrochromic bandshift
  • Non-photochemical quenching
  • P700
  • PSI photoinactivation
  • Transthylakoid proton gradient

ASJC Scopus subject areas

  • Biochemistry
  • Plant Science
  • Cell Biology

Cite this

Repetitive light pulse-induced photoinhibition of photosystem i severely affects CO2 assimilation and photoprotection in wheat leaves. / Zivcak, Marek; Brestic, Marian; Kunderlikova, Kristyna; Sytar, Oksana; Allakhverdiev, Suleyman.

In: Photosynthesis Research, Vol. 126, No. 2-3, 01.12.2015, p. 449-463.

Research output: Contribution to journalArticle

Zivcak, Marek ; Brestic, Marian ; Kunderlikova, Kristyna ; Sytar, Oksana ; Allakhverdiev, Suleyman. / Repetitive light pulse-induced photoinhibition of photosystem i severely affects CO2 assimilation and photoprotection in wheat leaves. In: Photosynthesis Research. 2015 ; Vol. 126, No. 2-3. pp. 449-463.
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