Reperfusion-induced gene expression profiles in rat lung transplantation

Masaomi Yamane, Mingyao Liu, Hiroyuki Kaneda, Stefan Uhlig, Thomas K. Waddell, Shaf Keshavjee

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Ischemia-Reperfusion (I/R) injury after lung transplantation (LTx) can lead to significant morbidity and mortality in recipients. In an attempt to improve our understanding of molecular mechanisms of I/R injury, we examined the changes in gene expression levels in a rat lung transplant model using oligonucleotide microarrays. Lewis rat lung grafts were stored for 6 or 24 h followed by transplantation and reperfusion for 2 h. Lung tissues were taken before and after flushing the grafts, before implantation, and after 2 h of reperfusion. RNA samples were examined with AffymetrixR rat microarray chips and RT-PCR was performed to validate significant changes in gene expression. Microarray analysis showed 404 genes that were upregulated more than 2-fold after reperfusion compared to cold ischemic lungs, and 187 genes that were down-regulated. Using RT-PCR, we confirmed the response pattern of several specific gene transcripts from the microarray analysis. Among these, up-regulation in transcripts of transcription factors, adhesion molecules, pro-coagulant factors and pro-inflammatory cytokines were identified. The differential gene regulation during the I/R process can be considered as molecular signatures for the changes of cellular metabolism, functions and injury. Reperfusion-induced genes related to inflammatory response may contribute to graft dysfunction in LTx.

Original languageEnglish
Pages (from-to)2160-2169
Number of pages10
JournalAmerican Journal of Transplantation
Volume5
Issue number9
DOIs
Publication statusPublished - Sep 2005
Externally publishedYes

Fingerprint

Lung Transplantation
Transcriptome
Reperfusion
Transplants
Lung
Genes
Microarray Analysis
Reperfusion Injury
Gene Expression
Polymerase Chain Reaction
Coagulants
Oligonucleotide Array Sequence Analysis
Transcription Factors
Up-Regulation
Ischemia
Transplantation
RNA
Cytokines
Morbidity
Mortality

Keywords

  • Acute lung injury
  • Bioinformatics
  • Ischemia-reperfusion
  • Lung preservation
  • Microarray

ASJC Scopus subject areas

  • Immunology

Cite this

Reperfusion-induced gene expression profiles in rat lung transplantation. / Yamane, Masaomi; Liu, Mingyao; Kaneda, Hiroyuki; Uhlig, Stefan; Waddell, Thomas K.; Keshavjee, Shaf.

In: American Journal of Transplantation, Vol. 5, No. 9, 09.2005, p. 2160-2169.

Research output: Contribution to journalArticle

Yamane, Masaomi ; Liu, Mingyao ; Kaneda, Hiroyuki ; Uhlig, Stefan ; Waddell, Thomas K. ; Keshavjee, Shaf. / Reperfusion-induced gene expression profiles in rat lung transplantation. In: American Journal of Transplantation. 2005 ; Vol. 5, No. 9. pp. 2160-2169.
@article{2b32c70331864ae982489bb4e69139c6,
title = "Reperfusion-induced gene expression profiles in rat lung transplantation",
abstract = "Ischemia-Reperfusion (I/R) injury after lung transplantation (LTx) can lead to significant morbidity and mortality in recipients. In an attempt to improve our understanding of molecular mechanisms of I/R injury, we examined the changes in gene expression levels in a rat lung transplant model using oligonucleotide microarrays. Lewis rat lung grafts were stored for 6 or 24 h followed by transplantation and reperfusion for 2 h. Lung tissues were taken before and after flushing the grafts, before implantation, and after 2 h of reperfusion. RNA samples were examined with AffymetrixR rat microarray chips and RT-PCR was performed to validate significant changes in gene expression. Microarray analysis showed 404 genes that were upregulated more than 2-fold after reperfusion compared to cold ischemic lungs, and 187 genes that were down-regulated. Using RT-PCR, we confirmed the response pattern of several specific gene transcripts from the microarray analysis. Among these, up-regulation in transcripts of transcription factors, adhesion molecules, pro-coagulant factors and pro-inflammatory cytokines were identified. The differential gene regulation during the I/R process can be considered as molecular signatures for the changes of cellular metabolism, functions and injury. Reperfusion-induced genes related to inflammatory response may contribute to graft dysfunction in LTx.",
keywords = "Acute lung injury, Bioinformatics, Ischemia-reperfusion, Lung preservation, Microarray",
author = "Masaomi Yamane and Mingyao Liu and Hiroyuki Kaneda and Stefan Uhlig and Waddell, {Thomas K.} and Shaf Keshavjee",
year = "2005",
month = "9",
doi = "10.1111/j.1600-6143.2005.01017.x",
language = "English",
volume = "5",
pages = "2160--2169",
journal = "American Journal of Transplantation",
issn = "1600-6135",
publisher = "Wiley-Blackwell",
number = "9",

}

TY - JOUR

T1 - Reperfusion-induced gene expression profiles in rat lung transplantation

AU - Yamane, Masaomi

AU - Liu, Mingyao

AU - Kaneda, Hiroyuki

AU - Uhlig, Stefan

AU - Waddell, Thomas K.

AU - Keshavjee, Shaf

PY - 2005/9

Y1 - 2005/9

N2 - Ischemia-Reperfusion (I/R) injury after lung transplantation (LTx) can lead to significant morbidity and mortality in recipients. In an attempt to improve our understanding of molecular mechanisms of I/R injury, we examined the changes in gene expression levels in a rat lung transplant model using oligonucleotide microarrays. Lewis rat lung grafts were stored for 6 or 24 h followed by transplantation and reperfusion for 2 h. Lung tissues were taken before and after flushing the grafts, before implantation, and after 2 h of reperfusion. RNA samples were examined with AffymetrixR rat microarray chips and RT-PCR was performed to validate significant changes in gene expression. Microarray analysis showed 404 genes that were upregulated more than 2-fold after reperfusion compared to cold ischemic lungs, and 187 genes that were down-regulated. Using RT-PCR, we confirmed the response pattern of several specific gene transcripts from the microarray analysis. Among these, up-regulation in transcripts of transcription factors, adhesion molecules, pro-coagulant factors and pro-inflammatory cytokines were identified. The differential gene regulation during the I/R process can be considered as molecular signatures for the changes of cellular metabolism, functions and injury. Reperfusion-induced genes related to inflammatory response may contribute to graft dysfunction in LTx.

AB - Ischemia-Reperfusion (I/R) injury after lung transplantation (LTx) can lead to significant morbidity and mortality in recipients. In an attempt to improve our understanding of molecular mechanisms of I/R injury, we examined the changes in gene expression levels in a rat lung transplant model using oligonucleotide microarrays. Lewis rat lung grafts were stored for 6 or 24 h followed by transplantation and reperfusion for 2 h. Lung tissues were taken before and after flushing the grafts, before implantation, and after 2 h of reperfusion. RNA samples were examined with AffymetrixR rat microarray chips and RT-PCR was performed to validate significant changes in gene expression. Microarray analysis showed 404 genes that were upregulated more than 2-fold after reperfusion compared to cold ischemic lungs, and 187 genes that were down-regulated. Using RT-PCR, we confirmed the response pattern of several specific gene transcripts from the microarray analysis. Among these, up-regulation in transcripts of transcription factors, adhesion molecules, pro-coagulant factors and pro-inflammatory cytokines were identified. The differential gene regulation during the I/R process can be considered as molecular signatures for the changes of cellular metabolism, functions and injury. Reperfusion-induced genes related to inflammatory response may contribute to graft dysfunction in LTx.

KW - Acute lung injury

KW - Bioinformatics

KW - Ischemia-reperfusion

KW - Lung preservation

KW - Microarray

UR - http://www.scopus.com/inward/record.url?scp=27844597435&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=27844597435&partnerID=8YFLogxK

U2 - 10.1111/j.1600-6143.2005.01017.x

DO - 10.1111/j.1600-6143.2005.01017.x

M3 - Article

VL - 5

SP - 2160

EP - 2169

JO - American Journal of Transplantation

JF - American Journal of Transplantation

SN - 1600-6135

IS - 9

ER -