Reperfusion hastens appearance and extent of distribution of type I collagen in infarct zone: Immunohistochemical study in rat experimental infarction

S. Yamasaki, S. Kusachi, H. Moritani, J. Kondo, S. Hirohata, A. Tamura, T. Tsuji

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5 Citations (Scopus)


Background: The effects of reperfusion on time-dependent alteration of type I collagen have not been examined. Objectives: We compared the sequential changes in the appearance and distribution of type I collagen in reperfused infarct rat hearts to those in non-reperfused hearts. Methods: Using an experimental rat model of infarction, we performed immunohistochemical staining with a polyclonal antibody to type I collagen by the avidin-biotin-peroxidase method. Reperfusion was established after 2-h coronary ligation that produced complete necrosis of myocytes. Results: In reperfused hearts, type I collagen appeared in the peripheral zone of the infarct at day 2, which was 1 day earlier than in non-reperfused hearts. The extent of distribution of type I collagen in reperfused hearts was comparable to that observed approximately 1 day later in non-reperfused hearts. Conclusion: Reperfusion can accelerate collagen matrix formation compared with that in non-reperfused hearts after acute myocardial infarction.

Original languageEnglish
Pages (from-to)763-768
Number of pages6
JournalCardiovascular Research
Issue number5
Publication statusPublished - Jan 1 1995



  • Avidin-biotin-peroxidase method
  • Extracellular matrix
  • Myocardial fibrosis
  • Myocardial infarct healing
  • Rat, anesthetized
  • Reperfusion

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)

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