Reperfusion hastens appearance and extent of distribution of type I collagen in infarct zone

Immunohistochemical study in rat experimental infarction

S. Yamasaki, S. Kusachi, H. Moritani, J. Kondo, Satoshi Hirohata, A. Tamura, T. Tsuji

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Background: The effects of reperfusion on time-dependent alteration of type I collagen have not been examined. Objectives: We compared the sequential changes in the appearance and distribution of type I collagen in reperfused infarct rat hearts to those in non-reperfused hearts. Methods: Using an experimental rat model of infarction, we performed immunohistochemical staining with a polyclonal antibody to type I collagen by the avidin-biotin-peroxidase method. Reperfusion was established after 2-h coronary ligation that produced complete necrosis of myocytes. Results: In reperfused hearts, type I collagen appeared in the peripheral zone of the infarct at day 2, which was 1 day earlier than in non-reperfused hearts. The extent of distribution of type I collagen in reperfused hearts was comparable to that observed approximately 1 day later in non-reperfused hearts. Conclusion: Reperfusion can accelerate collagen matrix formation compared with that in non-reperfused hearts after acute myocardial infarction.

Original languageEnglish
Pages (from-to)763-768
Number of pages6
JournalCardiovascular Research
Volume30
Issue number5
DOIs
Publication statusPublished - 1995

Fingerprint

Collagen Type I
Infarction
Reperfusion
Avidin
Biotin
Muscle Cells
Peroxidase
Ligation
Theoretical Models
Necrosis
Collagen
Myocardial Infarction
Staining and Labeling
Antibodies

Keywords

  • Avidin-biotin-peroxidase method
  • Extracellular matrix
  • Myocardial fibrosis
  • Myocardial infarct healing
  • Rat, anesthetized
  • Reperfusion

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Reperfusion hastens appearance and extent of distribution of type I collagen in infarct zone : Immunohistochemical study in rat experimental infarction. / Yamasaki, S.; Kusachi, S.; Moritani, H.; Kondo, J.; Hirohata, Satoshi; Tamura, A.; Tsuji, T.

In: Cardiovascular Research, Vol. 30, No. 5, 1995, p. 763-768.

Research output: Contribution to journalArticle

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AU - Yamasaki, S.

AU - Kusachi, S.

AU - Moritani, H.

AU - Kondo, J.

AU - Hirohata, Satoshi

AU - Tamura, A.

AU - Tsuji, T.

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N2 - Background: The effects of reperfusion on time-dependent alteration of type I collagen have not been examined. Objectives: We compared the sequential changes in the appearance and distribution of type I collagen in reperfused infarct rat hearts to those in non-reperfused hearts. Methods: Using an experimental rat model of infarction, we performed immunohistochemical staining with a polyclonal antibody to type I collagen by the avidin-biotin-peroxidase method. Reperfusion was established after 2-h coronary ligation that produced complete necrosis of myocytes. Results: In reperfused hearts, type I collagen appeared in the peripheral zone of the infarct at day 2, which was 1 day earlier than in non-reperfused hearts. The extent of distribution of type I collagen in reperfused hearts was comparable to that observed approximately 1 day later in non-reperfused hearts. Conclusion: Reperfusion can accelerate collagen matrix formation compared with that in non-reperfused hearts after acute myocardial infarction.

AB - Background: The effects of reperfusion on time-dependent alteration of type I collagen have not been examined. Objectives: We compared the sequential changes in the appearance and distribution of type I collagen in reperfused infarct rat hearts to those in non-reperfused hearts. Methods: Using an experimental rat model of infarction, we performed immunohistochemical staining with a polyclonal antibody to type I collagen by the avidin-biotin-peroxidase method. Reperfusion was established after 2-h coronary ligation that produced complete necrosis of myocytes. Results: In reperfused hearts, type I collagen appeared in the peripheral zone of the infarct at day 2, which was 1 day earlier than in non-reperfused hearts. The extent of distribution of type I collagen in reperfused hearts was comparable to that observed approximately 1 day later in non-reperfused hearts. Conclusion: Reperfusion can accelerate collagen matrix formation compared with that in non-reperfused hearts after acute myocardial infarction.

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