TY - JOUR
T1 - Repair of oxidative cytosine damage by DNA glycosylases.
AU - Katafuchi, Atsushi
AU - Matsuo, Aya
AU - Terato, Hiroaki
AU - Ohyama, Yoshihiko
AU - Ide, Hiroshi
N1 - Copyright:
This record is sourced from MEDLINE/PubMed, a database of the U.S. National Library of Medicine
PY - 2003
Y1 - 2003
N2 - 5-Hydroxyuracil (HOU) and 5-hydroxycytosine (HOC) are major oxidative lesions of cytosine with mutagenic potentials. Therefore, HOU and HOC need to be removed from DNA to avoid mutation. In this study, oligonucleotide substrates containing HOU and HOC were synthesized by DNA polymerase reactions and tested for DNA glycosylases. Ung exhibited an extremely low activity for HOU as compared to uracil (U). In contrast, hSMUG1 excised HOU and U with a comparable efficiency. Ung and hSMUG1 did not excise HOC.
AB - 5-Hydroxyuracil (HOU) and 5-hydroxycytosine (HOC) are major oxidative lesions of cytosine with mutagenic potentials. Therefore, HOU and HOC need to be removed from DNA to avoid mutation. In this study, oligonucleotide substrates containing HOU and HOC were synthesized by DNA polymerase reactions and tested for DNA glycosylases. Ung exhibited an extremely low activity for HOU as compared to uracil (U). In contrast, hSMUG1 excised HOU and U with a comparable efficiency. Ung and hSMUG1 did not excise HOC.
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U2 - 10.1093/nass/3.1.269
DO - 10.1093/nass/3.1.269
M3 - Article
C2 - 14510484
AN - SCOPUS:0142243236
SP - 269
EP - 270
JO - Nucleic acids research. Supplement (2001)
JF - Nucleic acids research. Supplement (2001)
IS - 3
ER -