Renal interstitial fibrosis is reduced in angiotensin II type 1a receptor-deficient mice

M. Satoh, N. Kashihara, Y. Yamasaki, K. Maruyama, K. Okamoto, Y. Maeshima, H. Sugiyama, T. Sugaya, K. Murakami, H. Makino

Research output: Contribution to journalArticle

129 Citations (Scopus)

Abstract

Unilateral ureteral obstruction (UUO) results in tubulointerstitial fibrosis of the affected kidney by stimulating the renin-angiotensin system. This study established a UUO model in angiotensin type 1a receptor (AT1a) deficient (mutant) mice to elucidate the role of angiotensin II through AT1a on the fibrosis of the obstructed kidney (OBK). The relative volume of the tubulointerstitium was measured by an image analyzer; deposition of collagen types III and IV and monocyte/macrophage infiltration were histologically examined using specific antibodies. Also determined were the mRNA levels of transforming growth factor-β by Northern blot analysis. Nuclear factor-κB activity was assessed by gel shift assay. UUO in wild mice resulted in a marked expansion of relative volume of the tubulointerstitium, together with increased deposition of collagen types III and IV and number of infiltrated monocytes/macrophages in the interstitium, relative to sham-operated mice. In comparison, these changes were significantly lower in mutant mice with UUO. The mRNA level of transforming growth factor-β was significantly higher in the OBK of wild mice with UUO compared with sham-operated mice. In contrast, the increase in mRNA level in the OBK of mutant mice was significantly less than in wild mice. Finally, UUO resulted in activation of nuclear factor-κB in wild mice but was inhibited in the OBK of mutant mice. The results provide direct evidence that angiotensin II acting via the AT1a plays a pivotal role in the development of tubulointerstitial fibrosis in UUO.

Original languageEnglish
Pages (from-to)317-325
Number of pages9
JournalJournal of the American Society of Nephrology
Volume12
Issue number2
Publication statusPublished - Feb 14 2001
Externally publishedYes

ASJC Scopus subject areas

  • Nephrology

Fingerprint Dive into the research topics of 'Renal interstitial fibrosis is reduced in angiotensin II type 1a receptor-deficient mice'. Together they form a unique fingerprint.

  • Cite this

    Satoh, M., Kashihara, N., Yamasaki, Y., Maruyama, K., Okamoto, K., Maeshima, Y., Sugiyama, H., Sugaya, T., Murakami, K., & Makino, H. (2001). Renal interstitial fibrosis is reduced in angiotensin II type 1a receptor-deficient mice. Journal of the American Society of Nephrology, 12(2), 317-325.