Renal functional measurements in young rats with chronic inhibition of nitric oxide synthase

Hirokazu Tsukahara, Toshio Imura, Shinya Tsuchida, Mitsuko Nunose, Chikahide Hori, Masahiro Hiraoka, Fumitake Gejyo, Masakatsu Sudo

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The purpose of the present study was to examine renal functional changes caused by chronic blockade of nitric oxide (NO) synthesis in young rats. Two types of NO synthase inhibitor were used: NG-nitro-L-arginine methyl ester (L-NAME) as a non-selective inhibitor and aminoguanidine (AG) as a selective inhibitor of the inducible isoform. Oral administration of L-NAME (20–80 mg/dL of drinking water), not AG (400 mg/dL), for 4 weeks induced systemic hypertension in the treated rats. Both inhibitors caused a significant reduction in urinary excretion of NO2/NO3. Rats treated with L-NAME developed proteinuria and tubular enzymuria (high excretion of N-acetyl-β-D-glucosaminidase) in a dose-dependent fashion, with normal serum levels of creatinine, albumin and cholesterol. Chronic AG administration did not alter the urinary levels of protein and N-acetyl-β-D-glucosaminidase or serum laboratory values. Overall, these observations highlight the importance of the continuous generation of NO by the constitutive isoform in the control of vascular tone and the maintenance of renal glomerular and tubular function. Oral administration of L-NAME may serve as a model of chronic NO-deficient hypertension with renal injury in young rats. 1996 Japan Pediatric Society.

Original languageEnglish
Pages (from-to)614-618
Number of pages5
JournalPediatrics International
Volume38
Issue number6
DOIs
Publication statusPublished - Dec 1996

Keywords

  • Hypertension
  • Nitric oxide
  • Nitric oxide synthase inhibitor
  • Renal function
  • Renal injury

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health

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