TY - GEN
T1 - Removing mesenchymal cells from gland tissue on micro-patterned tissue culture dish
AU - Matsumoto, Takuya
AU - Aoyagi, Seiji
PY - 2012/12/1
Y1 - 2012/12/1
N2 - The simple and safety method for removing mesenchymal tissue from the extracted submandibular gland (SMG) tissue is required for the in vitro study of SMG tissue development. For this purpose, we fabricated polydimethylsiloxane (PDMS) tissue culture substrate having micropillar structure. By applying O 2 plasma treatment and fibronectin coating, the surface affinity of PDMS substrate for cell and tissue was improved. Moreover, the pillar structure showed more effective to remove the mesenchymal tissue from isolated SMG tissue. Thus, the micro-fabricated surface with suitable chemical and physical modification is effective to remove mesenchymal tissue from extracted SMG tissue.
AB - The simple and safety method for removing mesenchymal tissue from the extracted submandibular gland (SMG) tissue is required for the in vitro study of SMG tissue development. For this purpose, we fabricated polydimethylsiloxane (PDMS) tissue culture substrate having micropillar structure. By applying O 2 plasma treatment and fibronectin coating, the surface affinity of PDMS substrate for cell and tissue was improved. Moreover, the pillar structure showed more effective to remove the mesenchymal tissue from isolated SMG tissue. Thus, the micro-fabricated surface with suitable chemical and physical modification is effective to remove mesenchymal tissue from extracted SMG tissue.
UR - http://www.scopus.com/inward/record.url?scp=84876513631&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84876513631&partnerID=8YFLogxK
U2 - 10.1109/MHS.2012.6492441
DO - 10.1109/MHS.2012.6492441
M3 - Conference contribution
AN - SCOPUS:84876513631
SN - 9781467348126
T3 - 2012 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2012
SP - 175
EP - 177
BT - 2012 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2012
T2 - 23rd Annual Symposium on Micro-Nano Mechatronics and Human Science, MHS 2012
Y2 - 4 November 2012 through 7 November 2012
ER -