Removing mesenchymal cells from gland tissue on micro-patterned tissue culture dish

Takuya Matsumoto, Seiji Aoyagi

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

The simple and safety method for removing mesenchymal tissue from the extracted submandibular gland (SMG) tissue is required for the in vitro study of SMG tissue development. For this purpose, we fabricated polydimethylsiloxane (PDMS) tissue culture substrate having micropillar structure. By applying O 2 plasma treatment and fibronectin coating, the surface affinity of PDMS substrate for cell and tissue was improved. Moreover, the pillar structure showed more effective to remove the mesenchymal tissue from isolated SMG tissue. Thus, the micro-fabricated surface with suitable chemical and physical modification is effective to remove mesenchymal tissue from extracted SMG tissue.

Original languageEnglish
Title of host publication2012 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2012
Pages175-177
Number of pages3
DOIs
Publication statusPublished - Dec 1 2012
Event23rd Annual Symposium on Micro-Nano Mechatronics and Human Science, MHS 2012 - Nagoya, Japan
Duration: Nov 4 2012 → Nov 7 2012

Publication series

Name2012 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2012

Other

Other23rd Annual Symposium on Micro-Nano Mechatronics and Human Science, MHS 2012
CountryJapan
CityNagoya
Period11/4/12 → 11/7/12

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Mechanical Engineering

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