Removing mesenchymal cells from gland tissue on micro-patterned tissue culture dish

Takuya Matsumoto; Seiji Aoyagi

doi:10.1109/MHS.2012.6492441

Removing mesenchymal cells from gland tissue on micro-patterned tissue culture dish

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

The simple and safety method for removing mesenchymal tissue from the extracted submandibular gland (SMG) tissue is required for the in vitro study of SMG tissue development. For this purpose, we fabricated polydimethylsiloxane (PDMS) tissue culture substrate having micropillar structure. By applying O ₂ plasma treatment and fibronectin coating, the surface affinity of PDMS substrate for cell and tissue was improved. Moreover, the pillar structure showed more effective to remove the mesenchymal tissue from isolated SMG tissue. Thus, the micro-fabricated surface with suitable chemical and physical modification is effective to remove mesenchymal tissue from extracted SMG tissue.

Original language	English
Title of host publication	2012 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2012
Pages	175-177
Number of pages	3
DOIs	https://doi.org/10.1109/MHS.2012.6492441
Publication status	Published - 2012
Event	23rd Annual Symposium on Micro-Nano Mechatronics and Human Science, MHS 2012 - Nagoya, Japan Duration: Nov 4 2012 → Nov 7 2012

Other

Other	23rd Annual Symposium on Micro-Nano Mechatronics and Human Science, MHS 2012
Country	Japan
City	Nagoya
Period	11/4/12 → 11/7/12

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ASJC Scopus subject areas

Electrical and Electronic Engineering
Mechanical Engineering

Cite this

Matsumoto, T., & Aoyagi, S. (2012). Removing mesenchymal cells from gland tissue on micro-patterned tissue culture dish. In 2012 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2012 (pp. 175-177). [6492441] https://doi.org/10.1109/MHS.2012.6492441

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Access to Document

10.1109/MHS.2012.6492441