Relevance of renal-specific oxidoreductase in tubulogenesis during mammalian nephron development

Yashpal S. Kanwar, Qiwei Yang, Yufeng Tian, Sun Lin, Jun Wada, Sumant Chugh, Satish K. Srivastava

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Renal-specific oxidoreductase (RSOR), an enzyme relevant to diabetic nephropathy, is exclusively expressed in renal tubules. Studies were initiated to determine whether, like other tubule-specific proteins, it selectively modulates tubulogenesis. Northern blot analyses revealed a ∼1.5-kb transcript, and RSOR expression was detectable in mice embryonic kidneys at day 13, gradually increased by day 17, and extended into neo- and postnatal periods. RSOR mRNA and protein expression was confined to proximal tubules, commencing at gestational day 17 and increasing subsequently, but remained absent in glomeruli and medulla. Treatment with RSOR antisense oligodeoxynucleotide resulted in a dose-dependent dysmorphogenesis of metanephric explants harvested at gestational day 13. The explants were smaller and had expanded mesenchyme, and the population of tubules was markedly decreased. The glomeruli were unaffected, as assessed by mRNA expression of glomerular epithelial protein 1 and reactivity with wheat germ agglutinin. Antisense treatment led to a selective reduction of RSOR mRNA. Immunoprecipitation also indicated a selective translational blockade of RSOR. These findings suggest that RSOR is developmentally regulated, exhibits a distinct spatiotemporal distribution, and probably plays a role in tubulogenesis.

Original languageEnglish
Pages (from-to)F752-F762
JournalAmerican Journal of Physiology - Renal Physiology
Volume282
Issue number4 51-4
DOIs
Publication statusPublished - 2002

Keywords

  • Diabetes mellitus
  • Nephrogenesis

ASJC Scopus subject areas

  • Physiology
  • Urology

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