Release of a damaged cofactor from a coenzyme B12-dependent enzyme: X-ray structures of diol dehydratase-reactivating factor

Naoki Shibata, Koichi Mori, Naoki Hieda, Yoshiki Higuchi, Mamoru Yamanishi, Tetsuo Toraya

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

The crystal structures of ADP bound and nucleotide-free forms of molecular chaperone-like diol dehydratase-reactivating factor (DDR) were determined at 2.0 and 3.0 Å, respectively. DDR exists as a dimer of heterodimer (αβ)2. The α subunit has four domains: ATPase domain, swiveling domain, linker domain, and insert domain. The β subunit, composed of a single domain, has a similar fold to the β subunit of diol dehydratase (DD). The binding of an ADP molecule to the nucleotide binding site of DDR causes a marked conformational change of the ATPase domain of the α subunit, which would weaken the interactions between the DDR α and β subunits and make the displacement of the DDR β subunit by DD through the β subunit possible. The binding of the DD β subunit to the DDR α subunit induces steric repulsion between the DDR α and DD α subunits that would lead to the release of a damaged cofactor from inactivated holoDD.

Original languageEnglish
Pages (from-to)1745-1754
Number of pages10
JournalStructure
Volume13
Issue number12
DOIs
Publication statusPublished - Dec 2005

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Propanediol Dehydratase
X-Rays
Enzymes
Adenosine Diphosphate
Adenosine Triphosphatases
cobamamide
Nucleotides
Molecular Chaperones

ASJC Scopus subject areas

  • Molecular Biology
  • Structural Biology

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Release of a damaged cofactor from a coenzyme B12-dependent enzyme : X-ray structures of diol dehydratase-reactivating factor. / Shibata, Naoki; Mori, Koichi; Hieda, Naoki; Higuchi, Yoshiki; Yamanishi, Mamoru; Toraya, Tetsuo.

In: Structure, Vol. 13, No. 12, 12.2005, p. 1745-1754.

Research output: Contribution to journalArticle

Shibata, Naoki ; Mori, Koichi ; Hieda, Naoki ; Higuchi, Yoshiki ; Yamanishi, Mamoru ; Toraya, Tetsuo. / Release of a damaged cofactor from a coenzyme B12-dependent enzyme : X-ray structures of diol dehydratase-reactivating factor. In: Structure. 2005 ; Vol. 13, No. 12. pp. 1745-1754.
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