Relationships between Cell Cycle Regulator Gene Copy Numbers and Protein Expression Levels in Schizosaccharomyces pombe

Ayako Chino, Koji Makanae, Hisao Moriya

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

We previously determined the copy number limits of overexpression for cell division cycle (cdc) regulatory genes in the fission yeast Schizosaccharomyces pombe using the "genetic tug-of-war" (gTOW) method. In this study, we measured the levels of tandem affinity purification (TAP)-tagged target proteins when their copy numbers are increased in gTOW. Twenty analyzed genes showed roughly linear correlations between increased protein levels and gene copy numbers, which suggested a general lack of compensation for gene dosage in S. pombe. Cdc16 and Sid2 protein levels but not their mRNA levels were much lower than that expected by their copy numbers, which suggested the existence of a post-transcriptional down regulation of these genes. The cyclin Cig1 protein level and its mRNA level were much higher than that expected by its copy numbers, which suggested a positive feedback mechanism for its expression. A higher Cdc10 protein level and its mRNA level, probably due to cloning its gene into a plasmid, indicated that Cdc10 regulation was more robust than that previously predicted.

Original languageEnglish
Article numbere73319
JournalPLoS One
Volume8
Issue number9
DOIs
Publication statusPublished - Sep 3 2013

Fingerprint

gene dosage
cdc Genes
Gene Dosage
Schizosaccharomyces
Schizosaccharomyces pombe
Regulator Genes
regulator genes
cell cycle
protein synthesis
Genes
Cells
Proteins
proteins
Messenger RNA
Cyclins
cyclins
Cloning
cell division
Organism Cloning
molecular cloning

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Relationships between Cell Cycle Regulator Gene Copy Numbers and Protein Expression Levels in Schizosaccharomyces pombe. / Chino, Ayako; Makanae, Koji; Moriya, Hisao.

In: PLoS One, Vol. 8, No. 9, e73319, 03.09.2013.

Research output: Contribution to journalArticle

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