Relationship between TNF-α and TUNEL-positive chondrocytes in antigen-induced arthritis of the rabbit temporomandibular joint

Yuriko Hirota, Manabu Habu, Kazuhiro Tominaga, Miho Sukedai, Akihiro Matsukawa, Tatsuji Nishihara, Jinichi Fukuda

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

BACKGROUND: Terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate biotin nick end-labeling (TUNEL) staining is a widely accepted method for the detection of DNA fragmentation in nuclei of apoptotic cells. Tumor necrosis factor (TNF)-α is closely associated with changes in condylar cartilage and modulates apoptosis in various tissues including cartilage. The aim of this study was to investigate the relationship between apoptotic chondrocytes and TNF-α in a rabbit model of arthritis. METHOD: Unilateral temporomandibular joint (TMJ) arthritis was induced in 20 adult New Zealand White rabbits. From 1 day to 6 weeks after the induction of arthritis, immunohistochemical analysis for TNF-α and TUNEL was performed. RESULTS: In condylar cartilage, TNF-α-positive cells and TUNEL-positive cells were localized together. TNF-α-positive chondrocytes seemed to precede TUNEL-positive cells. CONCLUSIONS: The results of the present study suggest that TNF-α may be involved in apoptosis and/or apoptotic necrosis of chondrocytes as TMJ arthritis progresses from the acute to chronic stage.

Original languageEnglish
Pages (from-to)91-98
Number of pages8
JournalJournal of Oral Pathology and Medicine
Volume35
Issue number2
DOIs
Publication statusPublished - Feb 1 2006
Externally publishedYes

Keywords

  • Antigen-induced arthritis
  • Apoptosis
  • Rabbit
  • Temporomandibular joint
  • Terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate biotin nick end-labeling
  • Tumor necrosis factor-α

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Oral Surgery
  • Otorhinolaryngology
  • Cancer Research
  • Periodontics

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