Relationship between the mobility of phosphocholine headgroup and the protein-liposome interaction: A dielectric spectroscopic study

Toshinori Shimanouchi, Noriko Yoshimoto, Azusa Hiroiwa, Keiichi Nishiyama, Keita Hayashi, Hiroshi Umakoshi

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Proteins could affect the headgroup mobility of phospholipid within liposome membranes through the protein-liposome interaction. The variation of headgroup mobility of phospholipid was then investigated by using the dielectric dispersion analysis. The eight proteins (Mw=4.2-28.7kDa) were used to investigate the protein-liposome interaction. It has been revealed that the strength of the protein-liposome interaction at 25°C was linearly correlated with the stability of intramolecular hydrogen bondings of proteins, better than with their hydrophobicity and the surface charge density. Overall, liposomes composed of binary lipid system, appeared to strongly interact with proteins, in contrast to liposomes composed of single, ternary, and quaternary lipid systems. This is probably because liposomes composed of binary lipid system favored to form the microscopic environment where proteins could interact. The present result suggested the heterogeneous phase state of lipid membranes was one of dominant factors for the interaction between proteins and lipid membranes.

Original languageEnglish
Pages (from-to)343-350
Number of pages8
JournalColloids and Surfaces B: Biointerfaces
Volume116
DOIs
Publication statusPublished - Apr 1 2014
Externally publishedYes

Fingerprint

Phosphorylcholine
Liposomes
proteins
Proteins
lipids
interactions
Lipids
Membrane Lipids
Phospholipids
Membrane Proteins
membranes
Hydrogen Bonding
Hydrophobic and Hydrophilic Interactions
Surface charge
Hydrophobicity
Charge density
hydrophobicity
Hydrogen bonds
Permittivity
Membranes

Keywords

  • Hydrogen bond
  • Lipid composition
  • Liposome
  • Protein

ASJC Scopus subject areas

  • Biotechnology
  • Colloid and Surface Chemistry
  • Physical and Theoretical Chemistry
  • Surfaces and Interfaces
  • Medicine(all)

Cite this

Relationship between the mobility of phosphocholine headgroup and the protein-liposome interaction : A dielectric spectroscopic study. / Shimanouchi, Toshinori; Yoshimoto, Noriko; Hiroiwa, Azusa; Nishiyama, Keiichi; Hayashi, Keita; Umakoshi, Hiroshi.

In: Colloids and Surfaces B: Biointerfaces, Vol. 116, 01.04.2014, p. 343-350.

Research output: Contribution to journalArticle

Shimanouchi, Toshinori ; Yoshimoto, Noriko ; Hiroiwa, Azusa ; Nishiyama, Keiichi ; Hayashi, Keita ; Umakoshi, Hiroshi. / Relationship between the mobility of phosphocholine headgroup and the protein-liposome interaction : A dielectric spectroscopic study. In: Colloids and Surfaces B: Biointerfaces. 2014 ; Vol. 116. pp. 343-350.
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