Relationship between SUMO-1 modification of caspase-7 and its nuclear localization in human neuronal cells

Naoko Hayashi, Hiromi Shirakura, Takashi Uehara, Yasuyuki Nomura

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

The aim of this study was to elucidate the role of SUMO-1 modification in caspase-7 in neuronal cells. We have previously demonstrated that procaspase-2 could be a possible target for SUMO-1 modification. In the present study, we attempted to investigate whether other caspases also interact with Ubc9/SUMO-1. The specific binding of SUMO-1 with caspase-7 was observed in mammalian cells. Deletion mutant analysis revealed that a SUMO-1 modification site may be located in at least N-terminal p20 subunit of caspase-7. Furthermore, SUMO-1-modified caspase-7 appeared as a dot-like structure in nuclear localization. These findings suggest that SUMO-1 modification in caspase-7 may be linked to specific its localization in the nucleus and may therefore contribute to the cleavage of nuclear substrates during neuronal apoptosis.

Original languageEnglish
Pages (from-to)5-9
Number of pages5
JournalNeuroscience Letters
Volume397
Issue number1-2
DOIs
Publication statusPublished - Apr 10 2006
Externally publishedYes

Fingerprint

Caspase 7
Caspase 2
Caspases
Apoptosis

Keywords

  • Apoptosis
  • Caspase
  • Nuclear localization
  • SUMO-1
  • Ubc9

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Relationship between SUMO-1 modification of caspase-7 and its nuclear localization in human neuronal cells. / Hayashi, Naoko; Shirakura, Hiromi; Uehara, Takashi; Nomura, Yasuyuki.

In: Neuroscience Letters, Vol. 397, No. 1-2, 10.04.2006, p. 5-9.

Research output: Contribution to journalArticle

Hayashi, Naoko ; Shirakura, Hiromi ; Uehara, Takashi ; Nomura, Yasuyuki. / Relationship between SUMO-1 modification of caspase-7 and its nuclear localization in human neuronal cells. In: Neuroscience Letters. 2006 ; Vol. 397, No. 1-2. pp. 5-9.
@article{278f182fb6984897914da0dcbe5f1b3f,
title = "Relationship between SUMO-1 modification of caspase-7 and its nuclear localization in human neuronal cells",
abstract = "The aim of this study was to elucidate the role of SUMO-1 modification in caspase-7 in neuronal cells. We have previously demonstrated that procaspase-2 could be a possible target for SUMO-1 modification. In the present study, we attempted to investigate whether other caspases also interact with Ubc9/SUMO-1. The specific binding of SUMO-1 with caspase-7 was observed in mammalian cells. Deletion mutant analysis revealed that a SUMO-1 modification site may be located in at least N-terminal p20 subunit of caspase-7. Furthermore, SUMO-1-modified caspase-7 appeared as a dot-like structure in nuclear localization. These findings suggest that SUMO-1 modification in caspase-7 may be linked to specific its localization in the nucleus and may therefore contribute to the cleavage of nuclear substrates during neuronal apoptosis.",
keywords = "Apoptosis, Caspase, Nuclear localization, SUMO-1, Ubc9",
author = "Naoko Hayashi and Hiromi Shirakura and Takashi Uehara and Yasuyuki Nomura",
year = "2006",
month = "4",
day = "10",
doi = "10.1016/j.neulet.2005.11.057",
language = "English",
volume = "397",
pages = "5--9",
journal = "Neuroscience Letters",
issn = "0304-3940",
publisher = "Elsevier Ireland Ltd",
number = "1-2",

}

TY - JOUR

T1 - Relationship between SUMO-1 modification of caspase-7 and its nuclear localization in human neuronal cells

AU - Hayashi, Naoko

AU - Shirakura, Hiromi

AU - Uehara, Takashi

AU - Nomura, Yasuyuki

PY - 2006/4/10

Y1 - 2006/4/10

N2 - The aim of this study was to elucidate the role of SUMO-1 modification in caspase-7 in neuronal cells. We have previously demonstrated that procaspase-2 could be a possible target for SUMO-1 modification. In the present study, we attempted to investigate whether other caspases also interact with Ubc9/SUMO-1. The specific binding of SUMO-1 with caspase-7 was observed in mammalian cells. Deletion mutant analysis revealed that a SUMO-1 modification site may be located in at least N-terminal p20 subunit of caspase-7. Furthermore, SUMO-1-modified caspase-7 appeared as a dot-like structure in nuclear localization. These findings suggest that SUMO-1 modification in caspase-7 may be linked to specific its localization in the nucleus and may therefore contribute to the cleavage of nuclear substrates during neuronal apoptosis.

AB - The aim of this study was to elucidate the role of SUMO-1 modification in caspase-7 in neuronal cells. We have previously demonstrated that procaspase-2 could be a possible target for SUMO-1 modification. In the present study, we attempted to investigate whether other caspases also interact with Ubc9/SUMO-1. The specific binding of SUMO-1 with caspase-7 was observed in mammalian cells. Deletion mutant analysis revealed that a SUMO-1 modification site may be located in at least N-terminal p20 subunit of caspase-7. Furthermore, SUMO-1-modified caspase-7 appeared as a dot-like structure in nuclear localization. These findings suggest that SUMO-1 modification in caspase-7 may be linked to specific its localization in the nucleus and may therefore contribute to the cleavage of nuclear substrates during neuronal apoptosis.

KW - Apoptosis

KW - Caspase

KW - Nuclear localization

KW - SUMO-1

KW - Ubc9

UR - http://www.scopus.com/inward/record.url?scp=33644810026&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33644810026&partnerID=8YFLogxK

U2 - 10.1016/j.neulet.2005.11.057

DO - 10.1016/j.neulet.2005.11.057

M3 - Article

C2 - 16378684

AN - SCOPUS:33644810026

VL - 397

SP - 5

EP - 9

JO - Neuroscience Letters

JF - Neuroscience Letters

SN - 0304-3940

IS - 1-2

ER -