Abstract
The aim of this study was to elucidate the role of SUMO-1 modification in caspase-7 in neuronal cells. We have previously demonstrated that procaspase-2 could be a possible target for SUMO-1 modification. In the present study, we attempted to investigate whether other caspases also interact with Ubc9/SUMO-1. The specific binding of SUMO-1 with caspase-7 was observed in mammalian cells. Deletion mutant analysis revealed that a SUMO-1 modification site may be located in at least N-terminal p20 subunit of caspase-7. Furthermore, SUMO-1-modified caspase-7 appeared as a dot-like structure in nuclear localization. These findings suggest that SUMO-1 modification in caspase-7 may be linked to specific its localization in the nucleus and may therefore contribute to the cleavage of nuclear substrates during neuronal apoptosis.
| Original language | English |
|---|---|
| Pages (from-to) | 5-9 |
| Number of pages | 5 |
| Journal | Neuroscience Letters |
| Volume | 397 |
| Issue number | 1-2 |
| DOIs | |
| Publication status | Published - Apr 10 2006 |
| Externally published | Yes |
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Keywords
- Apoptosis
- Caspase
- Nuclear localization
- SUMO-1
- Ubc9
ASJC Scopus subject areas
- Neuroscience(all)
Cite this
Relationship between SUMO-1 modification of caspase-7 and its nuclear localization in human neuronal cells. / Hayashi, Naoko; Shirakura, Hiromi; Uehara, Takashi; Nomura, Yasuyuki.
In: Neuroscience Letters, Vol. 397, No. 1-2, 10.04.2006, p. 5-9.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Relationship between SUMO-1 modification of caspase-7 and its nuclear localization in human neuronal cells
AU - Hayashi, Naoko
AU - Shirakura, Hiromi
AU - Uehara, Takashi
AU - Nomura, Yasuyuki
PY - 2006/4/10
Y1 - 2006/4/10
N2 - The aim of this study was to elucidate the role of SUMO-1 modification in caspase-7 in neuronal cells. We have previously demonstrated that procaspase-2 could be a possible target for SUMO-1 modification. In the present study, we attempted to investigate whether other caspases also interact with Ubc9/SUMO-1. The specific binding of SUMO-1 with caspase-7 was observed in mammalian cells. Deletion mutant analysis revealed that a SUMO-1 modification site may be located in at least N-terminal p20 subunit of caspase-7. Furthermore, SUMO-1-modified caspase-7 appeared as a dot-like structure in nuclear localization. These findings suggest that SUMO-1 modification in caspase-7 may be linked to specific its localization in the nucleus and may therefore contribute to the cleavage of nuclear substrates during neuronal apoptosis.
AB - The aim of this study was to elucidate the role of SUMO-1 modification in caspase-7 in neuronal cells. We have previously demonstrated that procaspase-2 could be a possible target for SUMO-1 modification. In the present study, we attempted to investigate whether other caspases also interact with Ubc9/SUMO-1. The specific binding of SUMO-1 with caspase-7 was observed in mammalian cells. Deletion mutant analysis revealed that a SUMO-1 modification site may be located in at least N-terminal p20 subunit of caspase-7. Furthermore, SUMO-1-modified caspase-7 appeared as a dot-like structure in nuclear localization. These findings suggest that SUMO-1 modification in caspase-7 may be linked to specific its localization in the nucleus and may therefore contribute to the cleavage of nuclear substrates during neuronal apoptosis.
KW - Apoptosis
KW - Caspase
KW - Nuclear localization
KW - SUMO-1
KW - Ubc9
UR - http://www.scopus.com/inward/record.url?scp=33644810026&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33644810026&partnerID=8YFLogxK
U2 - 10.1016/j.neulet.2005.11.057
DO - 10.1016/j.neulet.2005.11.057
M3 - Article
C2 - 16378684
AN - SCOPUS:33644810026
VL - 397
SP - 5
EP - 9
JO - Neuroscience Letters
JF - Neuroscience Letters
SN - 0304-3940
IS - 1-2
ER -