Relationship between SUMO-1 modification of caspase-7 and its nuclear localization in human neuronal cells

Naoko Hayashi, Hiromi Shirakura, Takashi Uehara, Yasuyuki Nomura

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

The aim of this study was to elucidate the role of SUMO-1 modification in caspase-7 in neuronal cells. We have previously demonstrated that procaspase-2 could be a possible target for SUMO-1 modification. In the present study, we attempted to investigate whether other caspases also interact with Ubc9/SUMO-1. The specific binding of SUMO-1 with caspase-7 was observed in mammalian cells. Deletion mutant analysis revealed that a SUMO-1 modification site may be located in at least N-terminal p20 subunit of caspase-7. Furthermore, SUMO-1-modified caspase-7 appeared as a dot-like structure in nuclear localization. These findings suggest that SUMO-1 modification in caspase-7 may be linked to specific its localization in the nucleus and may therefore contribute to the cleavage of nuclear substrates during neuronal apoptosis.

Original languageEnglish
Pages (from-to)5-9
Number of pages5
JournalNeuroscience Letters
Volume397
Issue number1-2
DOIs
Publication statusPublished - Apr 10 2006
Externally publishedYes

Keywords

  • Apoptosis
  • Caspase
  • Nuclear localization
  • SUMO-1
  • Ubc9

ASJC Scopus subject areas

  • Neuroscience(all)

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