Relationship between Streptococcus mutans expressing Cnm in the oral cavity and non-alcoholic steatohepatitis: A pilot study

Shuichi Tonomura, Shuhei Naka, Keiko Tabata, Tasuku Hara, Kojiro Mori, Saiyu Tanaka, Yoshio Sumida, Kazuyuki Kanemasa, Ryota Nomura, Michiyo Matsumoto-Nakano, Masafumi Ihara, Nobuyuki Takahashi, Kazuhiko Nakano

Research output: Contribution to journalArticle

Abstract

Background: Non-alcoholic steatohepatitis (NASH) is a severe state of non-alcoholic fatty liver disease (NAFLD), which is pathologically characterised by steatosis, hepatocyte ballooning, and lobular inflammation. Host-microbial interaction has gained attention as one of the risk factors for NASH. Recently, cnm-gene positive Streptococcus mutans expressing cell surface collagen-binding protein, Cnm (cnm-positive S. mutans), was shown to aggravate NASH in model mice. Here, we assessed the detection rate of cnm-positive S. mutans in oral samples from patients with NASH among NAFLD. Methods: This single hospital cohort study included 41 patients with NAFLD. NASH was diagnosed histologically or by clinical score. The prevalence of cnm-positive S. mutans, oral hygiene and blood tests, including liver enzymes, adipocytokines and inflammatory and fibrosis markers, were assessed in biopsy-proven or clinically suspected NASH among NAFLD. Results: Prevalence of cnm-positive S. mutans was significantly higher in patients with NASH than patients without NASH (OR 3.8; 95% CI 1.02 to 15.5). The cnm-positive S. mutans was related to decreased numbers of naturally remaining teeth and increased type IV collagen 7S level (median (IQR) 10.0 (5.0-17.5) vs 20.0 (5.0-25.0), p=0.06; 5.1 (4.0-7.9) vs 4.4 (3.7-5.3), p=0.13, respectively). Conclusions: Prevalence of cnm-positive S. mutans in the oral cavity could be related to fibrosis of NASH among NAFLD.

Original languageEnglish
Article numbere000329
JournalBMJ Open Gastroenterology
Volume6
Issue number1
DOIs
Publication statusPublished - Oct 1 2019

Fingerprint

Streptococcus mutans
Fatty Liver
Mouth
Fibrosis
Microbial Interactions
Adipokines
Collagen Type IV
Oral Hygiene
Hematologic Tests
Hepatocytes
Tooth
Carrier Proteins
Cohort Studies
Collagen
Non-alcoholic Fatty Liver Disease
Inflammation
Biopsy
Liver

Keywords

  • bacterial infection
  • infectious disease
  • nonalcoholic steatohepatitis

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Relationship between Streptococcus mutans expressing Cnm in the oral cavity and non-alcoholic steatohepatitis : A pilot study. / Tonomura, Shuichi; Naka, Shuhei; Tabata, Keiko; Hara, Tasuku; Mori, Kojiro; Tanaka, Saiyu; Sumida, Yoshio; Kanemasa, Kazuyuki; Nomura, Ryota; Matsumoto-Nakano, Michiyo; Ihara, Masafumi; Takahashi, Nobuyuki; Nakano, Kazuhiko.

In: BMJ Open Gastroenterology, Vol. 6, No. 1, e000329, 01.10.2019.

Research output: Contribution to journalArticle

Tonomura, S, Naka, S, Tabata, K, Hara, T, Mori, K, Tanaka, S, Sumida, Y, Kanemasa, K, Nomura, R, Matsumoto-Nakano, M, Ihara, M, Takahashi, N & Nakano, K 2019, 'Relationship between Streptococcus mutans expressing Cnm in the oral cavity and non-alcoholic steatohepatitis: A pilot study', BMJ Open Gastroenterology, vol. 6, no. 1, e000329. https://doi.org/10.1136/bmjgast-2019-000329
Tonomura, Shuichi ; Naka, Shuhei ; Tabata, Keiko ; Hara, Tasuku ; Mori, Kojiro ; Tanaka, Saiyu ; Sumida, Yoshio ; Kanemasa, Kazuyuki ; Nomura, Ryota ; Matsumoto-Nakano, Michiyo ; Ihara, Masafumi ; Takahashi, Nobuyuki ; Nakano, Kazuhiko. / Relationship between Streptococcus mutans expressing Cnm in the oral cavity and non-alcoholic steatohepatitis : A pilot study. In: BMJ Open Gastroenterology. 2019 ; Vol. 6, No. 1.
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abstract = "Background: Non-alcoholic steatohepatitis (NASH) is a severe state of non-alcoholic fatty liver disease (NAFLD), which is pathologically characterised by steatosis, hepatocyte ballooning, and lobular inflammation. Host-microbial interaction has gained attention as one of the risk factors for NASH. Recently, cnm-gene positive Streptococcus mutans expressing cell surface collagen-binding protein, Cnm (cnm-positive S. mutans), was shown to aggravate NASH in model mice. Here, we assessed the detection rate of cnm-positive S. mutans in oral samples from patients with NASH among NAFLD. Methods: This single hospital cohort study included 41 patients with NAFLD. NASH was diagnosed histologically or by clinical score. The prevalence of cnm-positive S. mutans, oral hygiene and blood tests, including liver enzymes, adipocytokines and inflammatory and fibrosis markers, were assessed in biopsy-proven or clinically suspected NASH among NAFLD. Results: Prevalence of cnm-positive S. mutans was significantly higher in patients with NASH than patients without NASH (OR 3.8; 95{\%} CI 1.02 to 15.5). The cnm-positive S. mutans was related to decreased numbers of naturally remaining teeth and increased type IV collagen 7S level (median (IQR) 10.0 (5.0-17.5) vs 20.0 (5.0-25.0), p=0.06; 5.1 (4.0-7.9) vs 4.4 (3.7-5.3), p=0.13, respectively). Conclusions: Prevalence of cnm-positive S. mutans in the oral cavity could be related to fibrosis of NASH among NAFLD.",
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T2 - A pilot study

AU - Tonomura, Shuichi

AU - Naka, Shuhei

AU - Tabata, Keiko

AU - Hara, Tasuku

AU - Mori, Kojiro

AU - Tanaka, Saiyu

AU - Sumida, Yoshio

AU - Kanemasa, Kazuyuki

AU - Nomura, Ryota

AU - Matsumoto-Nakano, Michiyo

AU - Ihara, Masafumi

AU - Takahashi, Nobuyuki

AU - Nakano, Kazuhiko

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N2 - Background: Non-alcoholic steatohepatitis (NASH) is a severe state of non-alcoholic fatty liver disease (NAFLD), which is pathologically characterised by steatosis, hepatocyte ballooning, and lobular inflammation. Host-microbial interaction has gained attention as one of the risk factors for NASH. Recently, cnm-gene positive Streptococcus mutans expressing cell surface collagen-binding protein, Cnm (cnm-positive S. mutans), was shown to aggravate NASH in model mice. Here, we assessed the detection rate of cnm-positive S. mutans in oral samples from patients with NASH among NAFLD. Methods: This single hospital cohort study included 41 patients with NAFLD. NASH was diagnosed histologically or by clinical score. The prevalence of cnm-positive S. mutans, oral hygiene and blood tests, including liver enzymes, adipocytokines and inflammatory and fibrosis markers, were assessed in biopsy-proven or clinically suspected NASH among NAFLD. Results: Prevalence of cnm-positive S. mutans was significantly higher in patients with NASH than patients without NASH (OR 3.8; 95% CI 1.02 to 15.5). The cnm-positive S. mutans was related to decreased numbers of naturally remaining teeth and increased type IV collagen 7S level (median (IQR) 10.0 (5.0-17.5) vs 20.0 (5.0-25.0), p=0.06; 5.1 (4.0-7.9) vs 4.4 (3.7-5.3), p=0.13, respectively). Conclusions: Prevalence of cnm-positive S. mutans in the oral cavity could be related to fibrosis of NASH among NAFLD.

AB - Background: Non-alcoholic steatohepatitis (NASH) is a severe state of non-alcoholic fatty liver disease (NAFLD), which is pathologically characterised by steatosis, hepatocyte ballooning, and lobular inflammation. Host-microbial interaction has gained attention as one of the risk factors for NASH. Recently, cnm-gene positive Streptococcus mutans expressing cell surface collagen-binding protein, Cnm (cnm-positive S. mutans), was shown to aggravate NASH in model mice. Here, we assessed the detection rate of cnm-positive S. mutans in oral samples from patients with NASH among NAFLD. Methods: This single hospital cohort study included 41 patients with NAFLD. NASH was diagnosed histologically or by clinical score. The prevalence of cnm-positive S. mutans, oral hygiene and blood tests, including liver enzymes, adipocytokines and inflammatory and fibrosis markers, were assessed in biopsy-proven or clinically suspected NASH among NAFLD. Results: Prevalence of cnm-positive S. mutans was significantly higher in patients with NASH than patients without NASH (OR 3.8; 95% CI 1.02 to 15.5). The cnm-positive S. mutans was related to decreased numbers of naturally remaining teeth and increased type IV collagen 7S level (median (IQR) 10.0 (5.0-17.5) vs 20.0 (5.0-25.0), p=0.06; 5.1 (4.0-7.9) vs 4.4 (3.7-5.3), p=0.13, respectively). Conclusions: Prevalence of cnm-positive S. mutans in the oral cavity could be related to fibrosis of NASH among NAFLD.

KW - bacterial infection

KW - infectious disease

KW - nonalcoholic steatohepatitis

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